EZ1 Advanced XL

从1–14个人类样本或法医检材样本中自动纯化核酸

S_1163_IAS_EZ1_0115

✓ 全天候自动处理在线订单

✓ 博学专业的产品和技术支持

✓ 快速可靠的(再)订购

EZ1 Advanced XL, Priority Package

Cat. No. / ID:  9001875

Instrument and service agreement package: robotic workstation for automated purification of nucleic acids from up to fourteen samples using EZ1 Kits; includes installation, application training, Full Agreement for two years with a two business day response time and two preventive maintenance visits.
InstrumentAccessories
EZ1 Advanced XL
Silicone Grease, BR M48 & BR EZ1
Service optionService Options
Priority Package
System
Priority Package Plus
System PRV-2
EZ1 Advanced XL应与QIAGEN试剂盒配合使用,适用于试剂盒用户手册中指定的应用。

✓ 全天候自动处理在线订单

✓ 博学专业的产品和技术支持

✓ 快速可靠的(再)订购

特点

  • 从多种样本中纯化高品质基因组DNA和RNA
  • 从各种生物医学研究样本中纯化得到病毒RNA/DNA和细菌DNA
  • 每次可处理至多14个样本
  • 全过程数据记录,数据管理简单方便
  • 紫外消毒,防止交叉感染

产品详情

EZ1 Advanced XL可从多种人类身份认定和法医检材样本、生物医学研究和基因表达分析的样本中纯化核酸。

绩效

标准化并可追踪

EZ1 Advanced XL建立数据管理的新标准。阅读条形码可在整个纯化过程中对样本和试剂进行全程追踪。可方便的输入备注和检测信息。试剂认证、批号和有效期同时登记,以提高过程控制和可靠度。如试剂超过有效期,后会发出警告。在个实验方案结束时会自动生成运行报告,包含维护过程和消毒日期等所有仪器信息。每次运行结束之后,仪器会生成一个pdf和一个csv文档。可便利的将数据传送至本地打印机或计算机上,报表可以用LIMS(实验室信息管理系统)处理。

经验证的技术、表现卓越

经验证的EZ1 Kits配合EZ1 Advanced XL Cards可确保表现卓越的纯化过程。EZ1 Advanced XL具有无与伦比的易用性,可快速纯化,并具有高度可重复性,无需特别培训仪器使用,也不要求具备操作经验。该仪器的应用范围可扩增,包括从血液、组织和遗传身份鉴定及法医鉴定样本中提取DNA,从细胞和组织中纯化RNA以及从各种样本类型中提取病毒核酸,可满足需要处理各种样本的实验室对于灵活性的要求。

安全性和可靠性

EZ1 Advanced XL具有独特的设计,可确保纯化过程高度安全、性能卓越并且使用简单。工作站可放置于小柜中,在必要的情况下确保用户安全。此外,配有可确保仪器的盖子在样本制备过程中关闭的传感器 ,减少实验室人员与可能感染的样本的接触,保护样本完整、不被污染。在每次运行结束后,智能的紫外线灯可对工作站表面进行消毒,减少不同批次间的样本污染。紫外线灭菌可高效消除革兰氏阳性菌和阴性菌(参见下表)。

E. coli菌群数量
照射时间(分钟) 照射次数 1 2 3 4 5 6
30 1 0 0 0 0 0 0
30 2 0 0 0 1 0 0
30 3 10 0 0 0 0 3
30 对照 311 352 367 367 351 396
60 1 0 0 0 0 0 0
60 2 0 0 0 0 0 0
60 3 0 0 0 0 0 0
60 对照 348 307 326 326 396 385
使用EZ1 Advanced的紫外灯照射6份同样的Escherichia coli培养液(每份20 μl ),之后在合适的条件下,在培养皿中进行培养。对每份培养皿进行不同次数的紫外灯照射。紫外线能高效杀灭细菌。
S. haemolyticus 菌群数量
照射时间(分钟) 照射次数 1 2 3 4 5 6
30 1 12 10 17 2 6 4
30 2 0 0 0 0 0 0
30 3 10 0 2 11 0 3
30 对照 248 256 396 314 321 267
60 1 0 0 0 0 0 0
60 2 0 0 0 0 0 0
60 3 10 0 0 0 0 0
60 对照 264 305 368 292 347 388
使用EZ1 Advanced的紫外灯照射6份同样的Staphylococcus haemolyticus培养液(每份20 μl ,浓度为15,700 CFU/ml),之后在合适的条件下,在培养皿中进行培养。对每份培养皿进行不同次数的紫外灯照射。紫外线能高效杀灭细菌。

原理

样本自动化制备可实现高效、标准化的核酸纯化,将手工操作可能引起的污染降至最低,确保用户安全和可靠的样本处理。

EZ1 Advanced XL配合经验证的EZ1 Kits,从各种样本中纯化DNA、RNA和病毒核酸,经纯化的核酸可直接用于下游应用。简单易用的工作站配合优化的实验方案和工作台设置、以及预制的密封试剂条可简化核酸纯化。

程序

灵活性、使用方便

EZ1 Advanced XL为用户带来便利的自动化工作站。提供多种适用于纯化DNA、RNA或病毒核酸的实验方案。EZ1 Advanced XL可在20分钟内纯化1–14个样本。该仪器通过键盘操作,VFD(真空荧光显示屏)上会显示仪器信息。为了方便根据不同样本制备的需求进行调整,可将多至4台EZ1 Advanced XL连接到一个外部电脑(QIAGEN提供),将通量提高至56个样本。

所有处理步骤均由仪器自动完成:包括穿刺试剂条,到洗脱高纯度核酸。无需使用另外的计算机,EZ1 Kits包括所有样本制备所需的试剂和附件。EZ1 Kits提供预装的、直至仪器门关闭开始运行都保持密封的试剂条,降低反应体系构建过程中的污染风险。

适用于多种应用的EZ1 Kits和Advanced XL Cards

EZ1 Advanced XL应用经验证的EZ1 Kits并配合预编在EZ1 Advanced XL Cards上的操作步骤。EZ1 Kits提供各种广泛的应用,包括遗传身份鉴定、法医鉴定、生物医学研究和基因表达分析等(参见下表)。

EZ1 Advanced Cards与EZ1 Kits
程序卡 试剂盒 样本
EZ1 Advanced XL DNA Blood Card EZ1 DNA Blood 200 µl  Kit 血液类样本
EZ1 Advanced XL DNA Blood Card EZ1 DNA Blood 350 µl Kit 血液类样本
EZ1 Advanced XL DNA Dried Blood Card EZ1 DNA Tissue Kit 干血
EZ1 Advanced XL DNA Buffy Coat Card EZ1 DNA Blood 350 µl Kit 白膜层
EZ1 Advanced XL DNA Tissue Card EZ1 DNA Tissue Kit 组织
EZ1 Advanced XL DNA Paraffin Section Card EZ1 DNA Tissue Kit 石蜡包埋组织
EZ1 Advanced XL DNA Buccal Swab Card EZ1 DNA Tissue Kit 口腔拭子
EZ1 Advanced XL DNA Investigator Card EZ1 DNA Investigator Kit 法医和人类身份认定样本
EZ1 Advanced XL DNA Bacteria Card EZ1 DNA Tissue Kit 人类样本、拭子、活检和细菌培养液
EZ1 Advanced XL Virus Card v2.0 EZ1 Virus Mini Kit v2.0 血清、血浆、脑脊液、尿液、全血、粪便、运输介质、呼吸道样本和干燥拭子样本中的病毒和细菌核酸
EZ1 Advanced XL RNA Card EZ1 RNA Universal Tissue Kit 各种人类或动物组织
EZ1 Advanced XL DSP DNA Blood Card* EZ1 DSP DNA Blood Kit* 人类全血
EZ1 Advanced XL DSP Virus Card* EZ1 DSP Virus Kit* 血清、血浆、脑脊液、尿液、全血、粪便、运输介质、呼吸道样本和干燥拭子样本中的病毒和细菌核酸
* EZ1 Advanced XL DSP Cards和EZ1 DSP Kits在欧洲、美国和加拿大可用于体外诊断。

应用

使用EZ1 Advanced XL纯化得到的核酸可即用于多种下游应用,包括:

  • 肿瘤、法医学研究和身份认定  
  • 基因分型
  • 传染病研究,包括细菌基因分型
  • 基因表达分析

服务

Have your throughput demands increased? Do you want to expand your application range or further streamline workflows? Do you need to manage more complex samples? Do you need improved connectivity?

If the answer to any of these questions is yes, then QIAGEN’s trade-in/trade-up program is just right for you!

QIAGEN makes it easy to keep up to speed with the latest automation and sample processing technologies. Simply exchange an old instrument for a new one. Improve your efficiency by trading in a competitor instrument or trading up with an older QIAGEN instrument.

Learn more about trade-in/trade-up opportunities.

辅助数据和图表

Specifications

FeaturesSpecifications
FeaturesTouch LCD user interface, IC card with protocols/software, barcode reader, UV lamp
Pipetting range50-1000 µl
Kits designed for this instrumentQIAGEN EZ1 Kits
TechnologyMagnetic-particle technology
Instrument dimensions510 x 510 x 570 mm (w x d x h)
Technical data of the instrumentWeight 48 kg, 100–240 V AC, 50–60 Hz
Protocols/main application on this instrumentPurification of DNA, mRNA, total RNA, and viral RNA and DNA
Samples per run (throughput)1–14 samples per run

服务计划

EZ1, Premium Agreement

Cat. No. / ID:  9239959

On-site EZ1 Advanced or EZ1 Advanced XL instrument repair, including travel, labor and parts, for a period of one year. Response time of next business day. Includes one Preventive Maintenance or Inspection Service during the Premium Agreement period.
EZ1, Full Agreement

Cat. No. / ID:  9237721

On-site EZ1 Advanced or EZ1 Advanced XL instrument repair, including travel, labor and parts, for a period of one year. Response time of two business days. Includes one Preventive Maintenance or Inspection Service during the Full Agreement period.

资源

用户使用手册 (1)
产品介绍与指南 (3)
Explore our new, easy-to-navigate digital Product Profile!
Advanced sample collection, automation and STR solutions for kinship testing
补充实验方案 (4)
Supplementary protocol using the EZ1 Advanced or EZ1 Advanced XL with the EZ1 DNA Investigator Kit
Supplementary protocol using the Investigator Lyse&Spin Basket Kit and EZ1 Advanced XL
Supplementary protocol using the Flip Cap Rack and the EZ1 Advanced XL Flip Cap Card.
安全数据表 (1)
Download Safety Data Sheets for QIAGEN product components.
验证报告 (1)
Safety Data Sheets (1)
Certificates of Analysis (1)

FAQ

What to do if I get error 24 (Open door in motion) on my EZ1 instruments?

Make sure a correct protocol card is inserted for the corresponding instrument. Protocol cards for BioRobot EZ1, EZ1 Advanced, and EZ1 Advanced XL cannot be interchanged. If you use an incorrect protocol card, you can get error 24.

If you are using a correct protocol card, please contact QIAGEN Technical Service.

FAQ ID -9128
What is the lifetime of the UV lamp on an EZ1 Advanced and EZ1 Advanced XL?

The lifetime of the UV lamps is 1200 cycles on an EZ1 Advanced.

The lifetime of the UV lamps is 1500 cycles on an EZ1 Advanced XL.

FAQ ID -9129
I observe more than normal magnetic bead carryover in my eluates after extraction on EZ1 instruments. What should I do?
  1. Make sure the maximum recommended sample amount is not exceeded.
  2. When using blood samples, make sure no clots are present.
  3. Make sure instrument is connected to a UPS (Uninterrupted Power Supply) to avoid power surge.
  4. Be sure to lubricate the O-rings of the tip adapters on the plunger unit, every week with the silicone grease (cat. no. 9239334). Refer to the user manual for instructions. Check that the tip adapters do not have excess grease.
  5. Perform a pipetting accuracy test using the appropriate Test Card for your instrument: EZ1 Test Card (cat. no. 9016187), EZ1 Advanced Test Card (cat. no. 9018300), or EZ1 Advanced XL Test Card (cat. no. 9018706).
  6. Teaching may have become corrupted. Check teaching. For instructions contact QIAGEN Technical Service.

If the issue persists, please contact QIAGEN Technical Service.

FAQ ID -9126
What to do if I get error 29 (Backup memory on the CPU is destroyed) on my EZ1 instrument?

          Do not remove protocol cards from the instrument while the instrument is switched on.

If the error 29 occurred on a BioRobot EZ1, you will need to clear the protocol memory and perform teaching. For instructions and teaching data sheet, contact QIAGEN Technical Service.

If the error 29 occurs on an EZ1 Advanced or an EZ1 Advanced XL, restart the instrument. The error should clear. If the error persists, contact QIAGEN Technical Service to obtain teaching instructions and teaching data sheet for your instrument.

 

FAQ ID -9122
What to do if I get error 25 (Abnormal input from bottom sensor in motion) on my EZ1 instrument?

Check that the recommended silicone grease (cat. no. 9239334) is used to lubricate the tip adapters on the plunger unit.

Make sure the tip adapters do not have excess grease. Wipe off any excess. Turn off the instrument and perform a test run to see if the error persists.

If correct grease was used and excess grease is not an issue, please contact QIAGEN Technical Service to obtain teaching instructions and teaching data sheet for your instrument.



FAQ ID -9121
I get “Master Data Error” after turning on my EZ1 instrument? What should I do?

Instrument seems to have lost teaching. Please contact QIAGEN Technical Service to obtain teaching instructions and teaching data sheet for your instrument.

If issue persists, a service visit may be needed.

FAQ ID -9127
What is the part number for transport lock or knurled knob for packing the EZ1 Advanced XL instrument?

 

Part number for transport lock for EZ1 Advanced XL is 9019723. You can place an order by calling your local QIAGEN customer care

FAQ ID -9125
What to do if I get error 13 (Failed to return to P axis, protocol in run) on my EZ1 instrument?

Instrument may have lost teaching. Please contact QIAGEN Technical Service to obtain teaching instructions and teaching data sheet for your instrument.

If issue persists, a service visit may be needed.

FAQ ID -9130
What to do if I get error 14 (Failed to return to M axis, protocol in run) on my EZ1 instrument?

Instrument may have lost teaching. Please contact QIAGEN Technical Service to obtain teaching instructions and teaching data sheet for your instrument.

If issue persists, a service visit may be needed.

FAQ ID -9132
What to do if I get error 19 (Y axis end limit, protocol in run) on my EZ1 instrument?

Check the workstation for any obstructions or any incorrect placement of the consumables (samples tubes, elution tubes, reagent cartridge, or tips).

If the issue persists, please contact QIAGEN Technical Service to obtain teaching instructions and teaching data sheet for your instrument.

FAQ ID -9120
I get “UV lamp did not ignite error” on my EZ1 Advanced and EZ1 Adavanced XL. What should I do?

UV lamp needs to be replaced. Please contact QIAGEN Technical Service to schedule service visit.

FAQ ID -9131
What to do if I get error 0182 (Attempt to move beyond the z-m axis, interference prevention value) on my EZ1 instrument?

 

Instrument may have lost teaching. Please contact QIAGEN Technical Service to obtain teaching instructions and teaching data sheet for your instrument. If teaching does not resolve the issue, a service visit may be needed.

FAQ ID -9123
What is the part number for silicone grease for greasing the O-ring of tip adapters on EZ1 series of instruments?

 The catalog number for Silicone grease for O-rings of Geno M6, M48, and EZ1 BioRobots is # 9239334. You can place an order by calling your local QIAGEN customer care.

FAQ ID -9124