FlexiTube siRNA Premix

用于优化的一步转染siRNA

产品

FlexiTube siRNA Premix 旨在用于分子生物学应用。该产品不能用于疾病诊断、预防和治疗。
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FlexiTube siRNA Premix

目录编号 / ID.   1027420

Optimized siRNA–reagent mix; 0.75 nmol siRNA; provided in tubes

特点

  • 加入到细胞中孵育即可,节省时间
  • 高效转染,减少手动操作
  • 多次实验均可获得可靠结果

产品详情

FlexiTube siRNA Premix是siRNA和转染试剂的混合物,通过预混siRNA和试剂,省去了混合及复合物形成所需的时间。此外,应用FlexiTube siRNA Premix无需计算siRNA与试剂的最佳比值。目前提供适用于人类和小鼠基因的FlexiTube siRNA Premix。

绩效

FlexiTube siRNA Premix中合理的试剂与siRNA比值,可提供高效的转染和基因抑制(参见" Rapid, efficient knockdown"和" Phenotype analysis after knockdown")。

FlexiTube siRNA Premix含有使用高度创新的HP OnGuard siRNA Design设计的FlexiTube siRNA,具有高效特异的优点。根据来自RNAi试验的数据建立极大容量的数据库,并在此基础上利用神经网络技术进行siRNAs设计,然后使用先进的、非冗余序列数据库和同源分析工具,检查该序列与同一基因组内其他序列的同源性。

性能保证

FlexiTube siRNA承诺提供一次性更换。如果订购了同一靶基因的多个FlexiTube siRNA Premixes,且至少2条未表现≥70%的基因抑制,QIAGEN将提供2条免费的额外siRNA,仅限一次。用户需要提供支持数据,以证明在适宜的转染条件下,siRNA未能成功地在mRNA水平抑制≥70%的靶基因。支持数据需要包括转染率数据,定量沉默数据以及≥70%的阳性对照抑制数据。该承诺自发货之日起6个月内有效。

原理

FlexiTube siRNA Premix的单个试管中预混有相关基因的siRNA和高效的转染试剂。该试剂盒同时含有可增强siRNA-试剂复合物稳定性的特殊缓冲液。siRNA-试剂的比值经过优化,可提供较高的转染率和基因抑制率。

程序

FlexiTube siRNA Premix可即时用于细胞转染,加入到细胞中孵育即可。 常规转染实验中siRNA被稀释后与转染试剂混合,然后孵育混合物以形成siRNA-试剂复合物,使用该试剂盒则只需将复合物加入到细胞中进行孵育,从而显著节省时间和人力。

使用FlexiTube siRNA Premix,无需优化siRNA-试剂比,可以更快的开展RNAi实验。该试剂盒提供siRNA和试剂的最佳比例预混物,最大程度减少或省去包括多重转染的冗长优化实验。

单个FlexiTube siRNA Premix可进行多重转染,降低变异性,确保了实验的一致性并使结果更加可靠。

应用

FlexiTube siRNA Premix是对少量靶基因进行功能基因组研究和通路分析的理想工具。

辅助数据和图表

规格

特点规格
DesignHP OnGuard design
Target sequence providedYes
ModificationNo
siRNA per target genevariable
FormatTube
Number of transfections50 standard transfections in 24-well format
Guarantee/validationfor validated siRNAs
SpeciesHuman, mouse
Scale or yield0.75 nmol

资源

安全数据表 (1)
Download Safety Data Sheets for QIAGEN product components.
Safety Data Sheets (1)
Certificates of Analysis (1)

常见问答

Is it possible to use lower siRNA concentrations with FlexiTube siRNA Premix?
FlexiTube siRNA Premix works with minimal siRNA concentrations. To decrease the final siRNA concentration, simply use smaller volumes of FlexiTube siRNA Premix for transfection. For example, using only half of the volume given in the protocol will lead to a final siRNA concentration of 12.5 nM. Depending on the cell type, final siRNA concentrations of down to 1 nM can lead to sufficient gene silencing.
FAQ-2264
If I am working with a difficult-to-transfect cell type or if I obtain only weak silencing effects what can I do?
In the case of difficult cell types or weak silencing effects, it may be helpful to increase the final siRNA concentration during transfection. This can be achieved simply by using larger amounts of FlexiTube siRNA Premix for transfection.
FAQ-2265
Do I have to order at least 4 FlexiTube siRNA Premixes?
No, there is no minimal order number for FlexiTube siRNA Premix. You can order one single FlexiTube siRNA Premix if desired.
FAQ-2266
Does the atypical look of the lyophilized FlexiTube siRNA Premix have a negative impact on performance?
The pellet of the lyophilized FlexiTube siRNA Premix does not look like a typical nucleic acid pellet.   The lyophilized FlexiTube siRNA Premix has a gel-like appearance.  This is the typical appearance of this product and has no impact on the quality of the material or its performance in downstream assays.
FAQ-2267
Is one able to use FlexiTube siRNA Premix for transfection of primary adherent cell types?
QIAGEN's experience using FlexiTube siRNA PreMix and transfection of primary adherent cell types is limited, but we do know that researchers have achieved excellent results with some primary cell types, for example, HUVEC cells.
FAQ-2268
Is one able to use FlexiTube siRNA Premix for transfection of primary suspension cell types?
Similar to other chemical transfection technologies, FlexiTube siRNA Premix is not recommended for the transfection of primary suspension cell types.
FAQ-2269
How do I store my FlexiTube siRNA Premix?
Reconstituted FlexiTube siRNA Premix can be stored at 4°C for one month.  For storage longer than one month, it is recommended to store the reconstituted mix at -20°C.  We found that after 5 freeze-thaw cycles, the potency of FlexiTube siRNA Premixes was not affected. However, although it is not recommended, we found that storage of reconstituted FlexiTube siRNA Premix for 6 months at 4°C did not reduce the gene silencing potency compared to reference transfections.
FAQ-2270
How do I submit a siRNA order by telephone or online?

FlexiTube siRNA, FlexiTube GeneSolution, FlexiTube siRNA Premix, FlexiPlate siRNA, and GeneFamily Lists siRNAs can be ordered by catalog number over the telephone.

However, to ensure accuracy, Custom siRNA Synthesis orders should be submitted in writing. Therefore you can use the HP Custom siRNA Order Form https://www.qiagen.com/products/genesilencing/customsirna/customsirnaorder.aspx?EmailOrdering=1.

Visit the RNAi Solutions page http://www.qiagen.com/products/rnai/default.aspx?r=2714 on our homepage for access to the Online Ordering Tool, and choose the order link for your product of interest.

FAQ-399
Has RNAi been successful using siRNA in Zebrafish and Xenopus?

Here are examples of references that describe the inhibition of gene expression by siRNA in Xenopus and Zebrafish:

FAQ-400