QIAsymphony DNA Investigator 试剂盒

用于在 QIAsymphony SP 仪器上从 1 至 96 个样本中自动纯化 DNA

S_1084_5_GEN_V2

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QIAsymphony DNA Investigator Kit (192)

Cat. No. / ID:   931436

用于 192 份 200 µl 案件和和参考样本制备 :包括 2 个试剂条、2 个酶架及附件
€1,267.00
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The QIAsymphony Investigator Kit is intended for molecular biology applications in forensic, human identity, and paternity testing. This product is not intended for diagnosis, prevention, or treatment of a disease.

✓ 全天候自动处理在线订单

✓ 博学专业的产品和技术支持

✓ 快速可靠的(再)订购

特点

  • 从法医和人类身份鉴定样本中纯化
  • 从痕量案件工作样本中获得更高效的产量
  • 信噪比更高
  • 新颖易用的预填充试剂卡夹
  • 通过标准化处理实现高重现性

产品详情

QIAsymphony DNA Investigator Kit 能在 QIAsymphony SP 上自动纯化来自 1-96 个样本(如拭子、过滤器、案件或犯罪现场样本和血液)的基因组 DNA。纯化效率高,并且纯化后的 DNA 在下游分析(如定量 PCR 和 STR 分析)中表现良好,信噪比高。QIAsymphony DNA Investigator Kit 符合 ISO 18385 要求。

绩效

QIAsymphony DNA Investigator Kit 能高效纯化 DNA,即使样本材料数量极少也可以。由于兼具高达 1000 µl 的样本输入量和低至 30 µl 的洗脱体积,因此能高灵敏地检测多种类型的样本,包括分散型斑迹。

原理

QIAsymphony DNA Investigator Kit 兼具硅基纯化基因组和线粒体 DNA 的速度和效率与磁珠微粒处理方便性(见“ 磁珠微粒的高效处理”图)。创新的即用型试剂卡夹预填充了磁珠和纯化程序所需的所有试剂,并由 QIAsymphony SP 自动打开,有助于将环境污染的风险降至最低(见“ 预填充密封试剂卡夹”图)。工作台设置迅速,可节省您的宝贵时间。只需将最多 2 个试剂卡夹放入“Reagents and Consumables”(试剂和耗材)抽屉。试剂卡盒可来自同一试剂盒或不同试剂盒,这样就可以在 96 个样本的同一运行过程中执行不同的纯化程序。只使用所选数量样本所需的试剂量,从而实现全面的成本控制。为了提高灵活性,DNA 可以在 30 至 400 µl 的水或 Buffer ATE(改良 TE 缓冲液)中进行洗脱。
查看图表

程序

纯化操作程序旨在确保安全且可重复地处理宝贵或可能有传染性的样本,包含 4 个步骤:裂解、结合、清洗和洗脱。用户可根据方案在 30 µl 到 400 µl 水或改良 TE 缓冲液 (Buffer ATE) 范围内选择不同的洗脱体积。

由于可以使用 QIAsymphony DNA Investigator Kit 处理的样本类型可能差异很大,因此还有各种不同的预处理,其已针对特定样本类型进行了优化。样本在存在蛋白酶 K 和 Buffer ATL 的变性条件下进行裂解,总体积为 200 µl、500 µl 或 1000 µl。

为了应对法医样本的特殊挑战,我们开发了样本制备方案,以确保尽可能高的一次通过成功率。高级方案 (ADV) 采用加热结合步骤并延长结合时间,以最大限度地从最难处理的样本中获得纯净 DNA。高效 (HE) 方案解决了从接触样本和其他低模板样本中回收足够 DNA 的难题。TopElute 液体可最大限度地提高对这些疑难样本的灵敏度。下表介绍了适用于不同参考和案件工作样本的全套高效法医方案。

 

QIAsymphony DNA Investigator 方案
方案 裂解体积选项 增强结合 Eluate volume(洗脱液体积) TopElute 液体
参比物质 200、500 µl 100–400 µl
案件工作 200、500、1000 µl 100–200 µl
Casework ADV 200、500、1000 µl 100–200 µl
Casework HE 200、500、1000 µl 30–80 µl
Casework HE ADV 200、500、1000 µl 30–80 µl

 

应用

使用 QIAsymphony DNA Investigator Kit 获得的高质量 DNA 适合直接用于法医、生物防御、生物安全以及其他人类身份鉴定应用的诸如以下活动:

  • 基因分型,包括指纹图谱和亲子鉴定分析
  • 从痕量样本中纯化 DNA(例如,犯罪现场)
  • 参考样本的常规分析

辅助数据和图表

Specifications

FeaturesSpecifications
ApplicationsSTR 分析、(实时)PCR
Yield< 3 µg
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein基因组 DNA、线粒体 DNA
Elution volume参考样本:100-400 µl;案件样本:30-200 µl
Sample type种类多样的法医和人类身份样本材料
Input volume参考样本:200 或 500 µl 裂解物;案件样本:200、500 或 1000 µl 裂解物
Technology磁珠技术

资源

实验方案软件 (2)
For use with the QIAsymphony SP/AS instruments (software version 5.0 or higher)
For use with the QIAsymphony SP/AS instruments (software version 5.0 or higher)
应用与实验方案 (14)
For use with software version 5.0
For use with software version 5.0
For use with software version 5.0
For use with software version 5.0 or higher
For use with software version 5.0 or higher
For use with software version 5.0 or higher
For use with software version 5.0
For use with software version 5.0
For use with software version 5.0 or higher
For use with software version 5.0
For use with software version 5.0 or higher
For use with software version 5.0
For use with software version 5.0
For use with software version 5.0 or higher
产品介绍与指南 (3)
Advanced sample collection, automation and STR solutions for kinship testing
安全数据表 (1)
Download Safety Data Sheets for QIAGEN product components.
应用说明 (2)

High-throughput DNA sample prep using the QIAsymphony SP instrument: An overview of workflow optimization at Bode Cellmark Forensics.

Labware Files and Documents (1)
For use with software version 5.0 or higher 
Technical Information and Important Notes (1)
仪器技术参数 (4)
QIAsymphony SP recovery procedures for DNA Investigator applications for software version 4.0 and HID 1.0
QIAsymphony SP recovery procedures for DNA Investigator applications for software version 4.0 and HID 1.0
QIAsymphony SP recovery procedures for DNA Investigator applications for software version 4.0 and HID 1.0
QIAsymphony SP recovery procedures for DNA Investigator applications for software version 4.0 and HID 1.0
技术资讯 (1)
Quality initiatives for human identity testing and forensics 
试剂盒操作手册 (1)
用户开发的实验方案 (1)

This protocol has been adapted from the pretreatment of sexual assault samples and is for the lysis and extraction of DNA from a sperm pellet after differential separation.

Safety Data Sheets (1)
Certificates of Analysis (1)
Application Notes (2)

High-throughput DNA sample prep using the QIAsymphony SP instrument: An overview of workflow optimization at Bode Cellmark Forensics.

Brochures & Guides (3)
Advanced sample collection, automation and STR solutions for kinship testing
Instrument Technical Documents (4)
QIAsymphony SP recovery procedures for DNA Investigator applications for software version 4.0 and HID 1.0
QIAsymphony SP recovery procedures for DNA Investigator applications for software version 4.0 and HID 1.0
QIAsymphony SP recovery procedures for DNA Investigator applications for software version 4.0 and HID 1.0
QIAsymphony SP recovery procedures for DNA Investigator applications for software version 4.0 and HID 1.0
Kit Handbooks (1)
Application/Protocol Documents (14)
For use with software version 5.0
For use with software version 5.0
For use with software version 5.0
For use with software version 5.0
For use with software version 5.0
For use with software version 5.0
For use with software version 5.0
For use with software version 5.0
For use with software version 5.0 or higher
For use with software version 5.0 or higher
For use with software version 5.0 or higher
For use with software version 5.0 or higher
For use with software version 5.0 or higher
For use with software version 5.0 or higher
Protocol Files (2)
For use with the QIAsymphony SP/AS instruments (software version 5.0 or higher)
For use with the QIAsymphony SP/AS instruments (software version 5.0 or higher)
Technical Information (1)
Quality initiatives for human identity testing and forensics 
User-Developed Protocols (1)

This protocol has been adapted from the pretreatment of sexual assault samples and is for the lysis and extraction of DNA from a sperm pellet after differential separation.

FAQ

Can DNA yields be increased by prolonging incubation with proteinase K in Buffer ATL?

For most sample types, incubating longer than specified in the pretreatment protocol for the QIAsymphony DNA Investigator Kit does not increase yields. However, lysates can be incubated overnight without negatively affecting the quality of the extracted DNA.

 

FAQ ID -2032
Can solid samples be processed with the QIAsymphony DNA Investigator Kit on the instrument?

No. All solid materials (e.g., swabs, blood card punches, chewing gum) must be removed completely from the lysate before performing automated extraction on the
QIAsymphony SP. The QIAshredder can be used to increase sample recovery from some solid materials. For more information, see the corresponding pretreatment protocol.

 

FAQ ID -2027
What is the composition of elution buffers used in QIAsymphony DNA Investigator kits?

User can choose elution with either buffer AVE or modified TE buffer known as buffer ATE.

* Buffer AVE contains RNase-free water with 0.04% NaN3 (Sodium azide).

* Buffer ATE contains 10 mM Tris-Cl pH 8.3, 0.1 mM EDTA and 0.04% NaN3 (Sodium-azide).

FAQ ID - 3387
Should sample lysate volumes be exactly 200 µL, 500 µL, or 1000 µL when loaded on the QIAsymphony SP?

No. The specified lysate volumes are optimal for QIAsymphony DNA Investigator Kit protocols. Lower volumes (150 µL,400 µL, 900 µL, respectively) can be used.

However, to maximize yields, we recommend transferring as much of the lysate as possible.
The QIAshredder can be used to increase recovery from some sample types. For more information, see the corresponding pretreatment protocol.

 

FAQ ID -2031
What kind of samples should be processed using the large-volume protocol with the QIAsymphony DNA Investigator Kit?

The large-volume protocol of the QIAsymphony DNA Investigator Kit is intended for samples that can not be submerged completely during lysis in volumes less than 1 ml, as well as samples that are very absorbent.

 

FAQ ID -2034
What is the maximum DNA yield from forensic samples processed with the QIAsymphony SP?

QIAsymphony DNA Investigator Kit protocols are optimized for maximum sensitivity in extraction of typical forensic-casework and reference sample types, which usually have low DNA content. Due to optimized bead capacity, maximum DNA yields are approximately 3 µg.

 

FAQ ID -2028
Do all QIAsymphony DNA Investigator protocols use carrier RNA?

No. Carrier RNA is only used in casework protocols for the QIAsymphony DNA Investigator Kit to enable efficient extraction of minute quantities of DNA. Carrier RNA is not used in reference protocols.

 

FAQ ID -2033
Why do casework-sample protocols for the QIAsymphony DNA Investigator Kit start with different lysate volumes (200 µl, 500 µl, or 1000 µl)?

To ensure performance with the QIAsymphony DNA Investigator Kit, lysis volumes have been optimized according to sample type. In general, large or absorbent samples require larger buffer volumes than small and non-absorbent samples.

 

FAQ ID -2035