Investigator IDplex Plus Kit

用于多重扩增 13 个 CODIS 核心基因座,外加 D2S1338、D19S433 和釉原蛋白基因,也用于直接从拭子和纸张进行多重扩增

S_1084_5_GEN_V2

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Investigator IDplex Plus Kit (100)

Cat. No. / ID:   381625

引物混合液、含 HotStarTaq Plus DNA 聚合酶的快速反应混合液、对照 DNA、等位基因分型标准物 IDplex Plus、DNA 分子量标准物 550 (BTO) 和无核酸酶水
€2,534.00
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KitAccessories
Investigator IDplex Plus
Matrix Standard
Reactions
100
400
本产品含有受 REACH 法规(EC 1907/2006 附录 XIV)管制的物质。允许在获得豁免的情况下(第 56(3) 条)在 EU 使用本产品。有关更多信息,请参阅 REACH 通知和本产品的 SDS(均可见于本页面“资源”部分)。
Investigator IDplex Kit 适用于法医鉴定、人类身份识别和亲子鉴定的分子生物学领域。这些产品不能用于疾病诊断、预防和治疗。这些产品并非在所有国家/地区都上市销售。

✓ 全天候自动处理在线订单

✓ 博学专业的产品和技术支持

✓ 快速可靠的(再)订购

特点

  • 同时扩增 16 个基因座,包括 CODIS 核心基因座
  • 非常适合法医鉴定案件或参考样本
  • 采用即用型 Fast Reaction Mix 的简单方案
  • 优化了引物设计以减少基因座重叠

产品详情

Investigator IDplex Plus Kit 是一种用于人类身份确定的多重 STR 检测试剂盒,涵盖 13 个 CODIS(DNA 联合索引系统)核心基因座、D2S1338、D19S433 和釉原蛋白基因。

该试剂盒利用我们的快速循环技术,可实现在大约 90 分钟内完成扩增。Investigator IDplex Plus Kit 具有高度灵敏度,可从痕量 DNA 中快速获得可靠的结果,适用于所有法医应用和亲子鉴定。基因分析仪使用 BT5 Matrix 进行校准。

Investigator IDplex Kit 符合 ISO 18385 要求。

绩效

Investigator IDplex Kit 利用快速循环技术进行 STR 检测,在大约 90 分钟内便可从提取的 DNA 中获得可靠的结果。

Fast Reaction Mix 基于久经考验的 QIAGEN 多重 PCR 技术,并对该技术进行优化,可以增强抑制物耐受性。在 6 种类型抑制物(腐殖酸、血红素、鞣酸、靛蓝胭脂红、钙和胶原蛋白)浓度增加的情况下测试的检测性能显示出高灵敏度和优异的抑制物耐受性。这使得能够从案件样本中获得可靠的高品质结果。

使用 GeneAmp 9700 热循环仪和 Applied Biosystems 3500 Genetic Analyzer 对 Investigator IDplex Kit 进行验证和评价。

在标准条件下,Investigator IDplex Plus Kit 的 DNA 推荐用量为 0.5 ng。内部验证已证实使用 0.2–2 ng DNA 可获得稳定、均衡的结果,使用 <0.1 ng DNA 可获得可靠的结果。

原理

Investigator IDplex Kit 使用 QIAGEN 的快速循环 PCR 技术同时对 13 个 CODIS(DNA 联合索引系统)核心基因座、D2S1338、D19S433 和釉原蛋白基因进行快速扩增。精心设计的引物减少了基因座等位基因的重叠,从而最大程度地减少了误读的可能性。

程序

15 种多态性高的常染色体 STR 基因座(D2S1338、D3S1358、D5S818、D7S820、D8S1179、D13S317、D16S539、D18S51、D19S433、D21S11、CSF1PO、FGA、TH01、TPOX 和 vWA)以及性别特异性釉原蛋白基因在单一 PCR 中同时扩增,并用“Investigator IDplex Plus Kit 染料及对应标志物”表中列出的某种染料进行标示。

 

Investigator IDplex Plus Kit 染料及对应标志物
染料 基因座
6-FAM Amelogenin TH01 D3S1358 vWA D21S11
BTG TPOX D7S820 D19S433 D5S818 D2S1338
BTY D16S539 CSF1PO D13S317 FGA  
BTR D18S51 D8S1179      

然后对扩增的基因座进行毛细管电泳。可以使用 GeneMapper ID-X 等专用软件分析结果。有关详细信息,请参阅“技术规格”表。

应用

Investigator IDplex Plus Kit 用于从血液、口腔拭子和法医学斑迹或参考样本中快速可靠地生成 DNA 图谱,非常适合:

  • 犯罪数据库、法医参考或案件样本
  • 亲子鉴定
  • 群体遗传学研究
  • 其他人类身份确定检测应用

 

技术规格
特点 Investigator IDplex Plus Kit
DNA 输入体积 最多 15 µl
每次反应的容量 25 µl
矩阵 BT5
荧光染料 6-FAM、BTG、BTY、BTO
基因分析仪 ABI PRISM 3100/3100 Avant、Applied
Biosystems 3130/3130xl 或 Applied
Biosystems 3500 Genetic Analyzer
分析软件 Applied Biosystems GeneMapper ID 或
GeneMapper ID-X

资源

产品介绍与指南 (2)
Advanced sample collection, automation and STR solutions for kinship testing
试剂盒操作手册 (1)
快速启动实验方案 (1)
模板文件 (3)
For further information on which version of the v11 template files is suitable for your needs, please refer to the Template File Info Note.
For further information on which version of the v11 template files is suitable for your needs, please refer to the Template File Info Note.
技术资讯 (3)

The most comprehensive and versatile coverage of current international STR standards for capillary electrophoresis analysis



Quality initiatives for human identity testing and forensics 
Technical Information and Important Notes (1)
安全数据表 (2)
Download Safety Data Sheets for QIAGEN product components.
Download Safety Data Sheets for QIAGEN product components.
Safety Data Sheets (1)
Certificates of Analysis (1)
Brochures & Guides (2)
Advanced sample collection, automation and STR solutions for kinship testing
Kit Handbooks (1)
Technical Information (3)

The most comprehensive and versatile coverage of current international STR standards for capillary electrophoresis analysis



Quality initiatives for human identity testing and forensics 
Template Files (3)
For further information on which version of the v11 template files is suitable for your needs, please refer to the Template File Info Note.
For further information on which version of the v11 template files is suitable for your needs, please refer to the Template File Info Note.
Quick-Start Protocols (1)

FAQ

Is the Fast Reaction Mix of the Investigator IDplex Plus Kit ready to use?
Yes. The Fast Reaction Mix of the Investigator IDplex Plus Kit contains HotStarTaq Plus enzyme, Mg2+, and dNTPs in a pre-optimized concentration. Only Investigator IDplex Plus Primer Mix and template DNA need to be added. Nuclease-free Water should be added to complete the reaction volume.
FAQ ID -3008
What is the safest procedure to add FTA punches to the PCR reactions of the Investigator IDplex GO! Kit?

We recommend first adding master mix to the PCR plate and then adding the punch to avoid punches getting lost or mixed up due to electrostatic effects. Furthermore, we strongly recommend spinning down the PCR plate after setup to ensure all punches get fully submerged.

FAQ ID -3096
Can the Investigator IDplex GO! PCR protocol be run on different PCR endpoint cyclers?
Yes. The protocol is generic and has successfully been tested on various commonly used instruments.
FAQ ID -3089
Can the PCR protocol of the Investigator IDplex Plus Kit be run on different PCR endpoint cyclers?
Yes. The protocol of the Investigator IDplex Plus Kit is generic and has been successfully tested on various commonly used instruments.
FAQ ID -3009
What is the maximum DNA input volume that can be used in the Investigator IDplex Plus Kit?
15 µl input volume can be used with the Investigator IDplex Plus Kit.
FAQ ID -3014
Can additional positive controls be used for the Investigator IDplex GO! Kit?

Amplification of Control DNA 9948 is appropriate as a PCR performance check. However, if you have access to reference samples at constant quality (for example, swabs or FTA donated by lab personnel), these samples may be used as additional in process controls.

FAQ ID -3094
Which filter set should be used with Investigator IDplex Plus Kit?
If you are using an ABI PRISM 3100-Avant/3100 or 3130/3130xl Genetic Analyzer, please use Any5Dye, if available. If you are using the Applied Biosystems 3500/3500xL Genetic Analyzer, please use filter set AnyDye. Otherwise, filter set G5 should be used. If you are using an ABI PRISM 310 Genetic Analyzer, filter set G5 should be used.
FAQ ID -3017
Does the Investigator IDplex Plus assay contain an internal control?
No. Unlike some other QIAGEN STR products (e.g., the Investigator Argus Y-12 QS Kit) there is no QS quality sensor included in the Investigator IDplex Plus Kit.
FAQ ID -3011
Is there a need to compensate for additional liquid volume added with the positive control DNA, or a buccal swab lysate in the Investigator IDplex GO! Kit?
No. Up to 2 µl can be added on top of a 25 µl reaction.
FAQ ID -3088
Is it possible to increase the amount of sample to obtain higher signals using the Investigator IDplex GO! Kit?
We do not recommend using more than one punch of 1.2 mm diameter for blood or buccal cells on FTA, or 2 µl of a crude buccal swab lysate. To adapt protocols to the type and quality of samples used in your laboratory, and to the CE instrumentation in use, adjust PCR cycle numbers and CE injection conditions.
FAQ ID -3090
Do you recommend using indicator cards for buccal cells on FTA with Investigator IDplex GO! Kit?
Yes. Use of indicator cards helps to ensure sample is taken from an appropriate area of the FTA card. This may significantly improve success rates compared to procedures using non-indicator cards.

 

FAQ ID -3095
What is the optimal sample DNA concentration for the Investigator IDplex Plus Kit?
The optimum sample DNA input range of the Investigator IDplex Plus Kit is between 200–500 pg. However, reliable results can be obtained with less than 100 pg. For higher amounts of input DNA, consider adjusting the PCR cycle number.
FAQ ID -3015
Can the allelic ladder of the Investigator IDplex Plus Kit be used for analysis of Investigator IDplex GO! Kit?

No. Amplification products of marker FGA have a slightly different size between IDplex Plus and IDplex GO! kits due to a change in the fluorescent dye linker used. Use of the IDplex Plus ladder will frequently cause off ladder calls for FGA alleles.

 

FAQ ID -3093
Is the Fast Reaction Mix ready to use in the Investigator IDplex GO?

Yes. The Fast Reaction Mix contains Taq enzyme, Mg2+, and dNTPs in a pre-optimized concentration. Only IDplex GO! Primer Mix and sample needs to be added.

FAQ ID -3087
Do I need to evaluate optimal PCR cycle numbers for the Investigator IDplex GO! Kit with my samples?

Yes. Sample materials used for direct amplification vary with regard to quality and quantity of DNA present, depending on sampling materials, procedures, and storage conditions. In order to obtain the highest possible success rates in routine use, it is essential to run a representative batch of samples for evaluation of optimal conditions. Furthermore, we recommend revisiting this aspect after a certain period of routine use to ensure the evaluated conditions are optimal and re-adjust if necessary.

FAQ ID -3091
Have primer sequences of the Investigator IDplex Plus Kit been changed compared to the former Investigator IDplex assay?
No, the primer site locations of the markers in the Investigator IDplex Plus Kit have not been changed. Only minor changes have been introduced for some markers, e.g., to improve A addition to the PCR products.
FAQ ID -3012
What is the correct matrix to use with Investigator IDplex Plus Kit?
Matrix BT5 should be used with Investigator IDplex Plus Kit.
FAQ ID -3016
What fluorescent labels are 6-FAM, BTG, BTY, BTR, and BTO in Matrix Standard BT5 single or multi.cap?

The BT5 matrix standard is designed for use with our 5-dye Investigator STR Assays. We do not disclose the containing dyes.

FAQ ID - 3364
Is it possible to extend the number of PCR cycles to increase sensitivity of the Investigator IDplex Plus Kit?
Yes. For example, cycle numbers can be increased to 32 or 34 in order to obtain higher signals for very diluted samples. However, please note that this promotes the amplification of nonspecific products or amplification from spurious background DNA.
FAQ ID -3010
What does the Investigator IDplex Plus Kit contain?
The Investigator IDplex Plus Kit contains Primer mix, Fast Reaction Mix, Control DNA allelic ladder IDplex Plus, DNA size standard 550 (BTO), and nuclease-free water.
FAQ ID -3013
Where can I find the template files for the Investigator IDplex Plus Kit?
The template files for the Investigator IDplex Plus Kit for various softwares and analyzers can be downloaded from the Resource tab on the product webpage.
FAQ ID -3019
Have primer sequences in the Investigator IDplex GO! Kit been changed compared to the Investigator IDplex Plus assay?

No, primer site locations of markers have not been changed. Two additional SNP primers have been added to allow balanced amplification of vWA and D16S539 alleles carrying known primer binding site mutations. The mutations covered were observed in concordance studies in samples of African origin at an elevated level. The labelled FGA forward primer has been slightly changed with regard to the linker of the fluorescent dye. The change does not affect PCR amplification, but causes a slight size shift during capillary electrophoresis. The allelic ladder therefore has been changed accordingly.

FAQ ID -3092
Which settings for the filter set Any5Dye must be adjusted before running the spectral calibration for Investigator IDplex Plus Kit?

For Any5Dye filter set with the Investigator IDplex Plus Kit, please use:

  • Condition Bound: Lower 1.0 – Upper 20
  • Sensitivity: 0.1
  • Quality Value: 0.80
FAQ ID -3018