QIAamp DNA Investigator Kit

用于手动或自动纯化法医和人类身份样本中的 DNA

S_1084_5_GEN_V2

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QIAamp DNA Investigator Kit (50)

Cat. No. / ID:  56504

用于 50 次 DNA 制备:50 QIAamp MinElute Columns, Proteinase K, Carrier RNA, Buffers, Collection Tubes (2 ml)
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QIAamp DNA Investigator Kit 旨在用于法医鉴定、人类身份识别、亲子鉴定等领域的分子生物学应用场景。该产品不能用于疾病诊断、预防和治疗。

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

  • 手动或在 QIAcube Connect 上纯化高质量的即用型 DNA
  • 稳定的高产量
  • 完全去除污染物和抑制剂

Product Details

QIAamp DNA Investigator Kit 可纯化多种法医和人类身份样本(如干血、骨骼和性侵样本、拭子和过滤器等案件工作样本)中的基因组 DNA。该试剂盒使用 QIAamp MinElute Spin Columns 来纯化高质量 DNA,洗脱体积灵活。纯化快速高效,并且纯化后的 DNA 在下游分析(如定量 PCR 和 STR 分析)中表现良好,信噪比高。使用 QIAamp DNA Investigator Kit 进行的 DNA 纯化可在 QIAcube Connect 上自动完成。QIAamp DNA Investigator Kit 符合 ISO 18385 要求。

Performance

QIAamp DNA Investigator Kit 适用于痕量样本(例如犯罪现场样本)的 DNA 纯化;基因分型,包括指纹图谱和亲子鉴定分析;以及参考样本的常规分析。

样品裂解后,QIAamp DNA Investigator 流程简单,非常适合用于同时处理多个样本,不到 30 分钟就能得到纯净的 DNA。DNA 在 Buffer ATE 或水中洗脱,可立即用于扩增反应或保存在 -20°C 下。纯化的 DNA 不含蛋白质、核酸酶和其他抑制剂。

Principle

QIAamp DNA Investigator Kit 将硅胶膜的选择性附着属性与 20 至 100 µl 的灵活洗脱体积相结合。

Procedure

QIAamp DNA Investigator 使用 QIAamp MinElute Spin Columns 纯化高质量 DNA,洗脱体积灵活,工作流程包括 4 个步骤(见“ 程序”流程图)。样本在变性条件下用蛋白酶 K 裂解。DNA 与膜结合后,污染物流过,残留污染物被洗去。浓缩的纯 DNA 从膜上洗脱下来。QIAamp DNA Investigator Kit 可在  QIAcube 上自动运行。

该程序旨在确保不存在样本间的交叉污染。

See figures

Applications

使用 QIAamp DNA Investigator Kit 获得的高质量 DNA 适合直接用于法医和人类身份鉴定领域的诸如以下应用:

  • 基因分型,包括指纹图谱和亲子鉴定分析
  • 从痕量样本中纯化 DNA(例如,犯罪现场)
  • 参考样本的常规分析

Supporting data and figures

Specifications

FeaturesSpecifications
Applications实时 PCR、STR 分析
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein基因组 DNA、线粒体 DNA
Sample types种类多样的法医和人类身份样本材料
Processing手动
Elution volume20-100 µl
Time per run or per prep不到 30 分钟
Technology硅胶技术
Format离心柱
Yield< 3 µg

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FAQ

I received a kit containing the MinElute columns; however, they were left out for a while and not stored at 2–8°C upon receipt. Can I still use them?

The MinElute spin columns included in the following kits should be stored at 2–8°C upon arrival: AllPrep DNA/RNA Micro, EpiTect Fast DNA Bisulfite, EpiTect Fast FFPE Bisulfite, EpiTect Fast LyseAll Bisulfite, EpiTect Plus DNA Bisulfite, EpiTect Plus FFPE Bisulfite, EpiTect Plus LyseAll Bisulfite, exoRNeasy Serum/plasma Maxi, exoRNeasy Serum/Plasma Midi, GeneRead DNA FFPE, GeneRead rRNA Depletion, GeneRead Size Selection, MinElute Gel Extraction, MinElute PCR Purification, MinElute Reaction Cleanup, miRNeasy FFPE, miRNeasy Micro, miRNeasy Serum/Plasma, QIAamp DNA FFPE, QIAamp DNA Investigator, QIAamp DNA Micro, QIAamp MinElute Media, QIAamp MinElute Virus Spin, QIAamp MinElute Virus Vacuum, RNeasy FFPE, RNeasy Micro, RNeasy Plus Micro.

Short-term storage (up to 4 weeks) at room temperature (15–25°C) does not affect the performance. However, for optimal performance and quality, storage temperature should not exceed 25°C.

FAQ ID - 3560
What is the composition of elution buffer ATE in the QIAamp DNA Investigator kit, QIAamp DNA FFPE Tissue kit and the QIAamp Fast DNA Stool Mini kit?

The composition of Buffer ATE is:

- 10 mM Tris-Cl pH 8.3

- 0.1 mM EDTA

- 0.04% NaN3 (sodium-azide)

FAQ ID -3122
What can be used as an alternative to the A260 measurement for quantification of small amounts of RNA and DNA?

Small amounts of RNA and DNA may be difficult to measure spectrophotometrically. Fluorometric measurements, or quantitative RT-PCR and PCR are more sensitive and accurate methods to quantify low amounts of RNA or DNA.

Fluorometric measurements are carried out using nucleic acid binding dyes, such as RiboGreen® RNA Quantitation Reagent for RNA, and PicoGreen® DNA Quantitation Reagent for DNA (Molecular Probes, Inc.).

FAQ ID -728