Investigator STAR Lyse&Prep Kit

用于使用开放式 DNA 提取平台从法医样本中自动纯化 DNA

S_5041_AT_InvestigatorSTAR_s

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Investigator STAR Lyse&Prep Kit (400)

Cat. No. / ID:  931447

用于从案件和参考样本制备 400 份样本:Buffer ATL、Buffer QSL3、Buffer QSW1、Buffer QSW2、Bead Suspension G、Buffer ATE、蛋白酶 K、载体 RNA、Q-Card
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Investigator STAR Lyse&Prep Kit 旨在用于法医鉴定、人类身份识别、亲子鉴定等领域的分子生物学应用场景。该产品不能用于疾病诊断、预防和治疗。

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

  • 从法医和人类身份鉴定样本中纯化
  • 兼容第三方开放式 DNA 提取平台
  • 从痕量案件样本获得高效产量
  • 专为在高通量实验室进行高效和安全的使用而设计
  • 包含用于裂解、结合、清洗和洗脱样本的所有试剂

Product Details

Investigator STAR Lyse&Prep Kit 利用 Hamilton、TECAN 等第三方供应商的开放式 DNA 提取平台实现自动纯化法医鉴定样本中的基因组 DNA。该试剂盒格式专为高通量应用而设计,可加速实验室工作流程并生产大量高质量 DNA 用于下游处理。Investigator STAR Lyse&Prep Kit 符合 ISO 18385 要求。

Performance

Investigator STAR Lyse&Prep Kit 可实现在高通量设置下高效纯化法医鉴定案例样本中的 DNA。该试剂盒包括足够用于 400 个样本的试剂,并且包括多瓶缓冲液和酶,经实测,每个样本制备批次利用一瓶,每瓶可实现多达 96 个样本的制备。这确保了试剂的高效利用和开封试剂的最低限度储存,从而降低任何污染风险。可以调整输入体积以适应较大样本的较大裂解体积和 Investigator Lyse&Spin Basket 裂解吊篮的使用。

Principle

Investigator STAR Lyse&Prep Kit 可重复地自动纯化法医和人类身份鉴定应用中的基因组 DNA。纯化过程高效,并且纯化的 DNA 在定量 PCR 和 STR 分析等下游分析中表现出色。它旨在确保安全且可重复地处理珍贵样本,包含 4 个步骤:裂解、结合、清洗和洗脱。
由于可以使用 Investigator STAR Lyse&Prep Kit 处理的样本类型可能差异很大,因此还有各种不同的预处理,它们针对特定样本类型进行了优化。在蛋白酶 K 和 Buffer ATL 的变性条件下,在 300 µl(适用于常规使用)或 500 µl(便于使用 Investigator Lyse&Spin Basket Kit 以及处理较大样本)的总体积中裂解样本。
磁珠技术将基于二氧化硅的 DNA 纯化的速度和效率与磁珠的方便处理相结合,采用专为开放式 DNA 提取平台设计的格式。在存在高离液盐的情况下,通过 DNA 与磁珠二氧化硅表面的结合,一步式从裂解物中分离 DNA。使用磁铁将磁珠与裂解物分离。然后对 DNA 进行高效清洗,并在改性的 TE 缓冲液 (Buffer ATE) 中洗脱。

Applications

使用 Investigator STAR Lyse&Prep Kit 获得的高质量 DNA 适合直接用于诸如以下应用的法医鉴定和人类身份识别检测:

  • 基因分型,包括指纹图谱和亲子鉴定分析
  • 从痕量样本中纯化 DNA(例如,犯罪现场)
  • 参考样本的常规分析

Supporting data and figures

Specifications

FeaturesSpecifications
ApplicationsSTR 分析、(实时)PCR
Sample type种类多样的法医和人类身份样本材料
Technology磁珠技术
Input volume300 或 500 µl 裂解物

Resources

安全数据表 (1)
Download Safety Data Sheets for QIAGEN product components.
快速启动实验方案 (1)
技术资讯 (2)
Quality initiatives for human identity testing and forensics 
试剂盒操作手册 (1)
Certificates of Analysis (1)
Technical Information (2)
Quality initiatives for human identity testing and forensics 
Kit Handbooks (1)
Quick-Start Protocols (1)

FAQ

What is the composition of the binding buffer?
The binding buffer is a mixture of QSL3 and QSW2. If using the 300 µL protocol, the ratio is 50:50 to make a total volume of 720 µL per sample. If using the 500 µL protocol, the ratio is 60:40 QSW2 to QSL3, respectively, up to 800 µL per sample.
FAQ ID - 4020
Can I use DNA-free water to elute my samples rather than the ATE buffer?
Yes, it’s possible, but best results are achieved using the supplied ATE buffer.
FAQ ID - 4022
Why is there difference in temperature for the wash steps between the manual and automated methods?
Automation has the convenience of temperature adjustment as part of the programmed steps. For added convenience while performing the method manually, the temperature does not need to be adjusted between the various protocol steps. The temperature during wash steps has no impact on the performance.
FAQ ID - 4024
After the air-drying steps, I can still see liquid. Should I continue to dry my samples?
Yes, the air-dry step is necessary to ensure that any residual ethanol from the QSW2 buffer evaporates away prior to continuing. Ethanol carry-over into the sample eluate is inhibitory to downstream PCR applications. Prior to the incubation step, ensure all liquid is removed using a pipette; you may need to aspirate more than once. Be careful not to disturb the magnetic beads.
FAQ ID - 4023
Can I make the binding buffer in advance?
For best results, prepare the binding buffer immediately before use. Gently mix the two reagents together in a suitable tube (e.g., a 50 mL conical tube) and gently mix by inverting 3–4 times. Do not shake vigorously as this can lead to excessive bubbling.
FAQ ID - 4021