EZ1 DNA Investigator Kit

从法医检材和人类身份鉴定样本中自动纯化DNA,每次可处理1–6个样本或1–14个样本

S_1084_5_GEN_V2

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EZ1&2 DNA Investigator Kit (48)

Cat. No. / ID:  952034

For 48 preps: Reagent Cartridges (DNA Investigator), Disposable Filter-Tips, Disposa-ble Tip-Holders, Sample Tubes (2 ml), Elution Tubes (1.5 ml), Buffer G2, Proteinase K, Carrier RNA
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KitProduct
EZ1&2 DNA Investigator Kit
7 mL Large-Volume Tubes (48)
The EZ1&2 DNA Investigator Kit is intended for molecular biology applications in forensic, human identity, and paternity testing. This product is not intended for diagnosis, prevention, or treatment of a disease.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

  • 痕量检材,更高得率
  • 更高信噪比
  • Tip dance方式处理固体样本
  • 可选择使用TE洗脱缓冲液及载体RNA

Product Details

EZ1 DNA Investigator Kit可从用于人类身份鉴定样本中自动纯化基因组DNA。该试剂盒可配合EZ1 Advanced XL(每批处理1–14个样本)、EZ1 Advanced(每批处理1–6个样本)和BioRobot EZ1(每批处理1–6个样本)工作站使用。纯化过程快速高效,产物DNA在各种下游分析中表现出色,例如定量PCR和STR分析,结果信噪比高。

Performance

更高的纯化效率,用于敏感的下游分析

EZ1 DNA Investigator Kit含有优化的试剂,可自动纯化获得高产量DNA。在STR分析中获得更高的峰值,提高检测灵敏度(参见" Improved performance in STR analysis")。具有更高的信噪比,确保下游应用中的高灵敏性和优越表现。比较STR分析中的峰高,潜在的DNA的回收率为79%(未显示)。使用TE缓冲液洗脱,结果相似,而且长时间贮存时有更高的稳定性。任何样本的处理过程无遗失(检查任意8个等位基因都有<50的相对荧光单位)。

不同实验方案STR的相对峰高值
实验 试剂盒和卡 方案 洗脱液 相对峰高值 遗漏数
1    EZ1 DNA Tissue Kit和EZ1 DNA Forensic Card 痕量 水  100% 0/8
1 EZ1 DNA Investigator Kit和Card 痕量 176% 0/8
2 EZ1 DNA Investigator Kit和Card 痕量 100% 0/8
2 EZ1 DNA Investigator Kit和Card 痕量 TE 116% 0/8
3 EZ1 DNA Investigator Kit和Card(200 µl) 痕量 100% 0/8
3 EZ1 DNA Investigator Kit和Card(500 µl) 大体积 203%(起始样本量的81% ) 0/8
AmpFlSTR处理的DNA由Buffer G2溶解,最终浓度为5 pg/µl,200 µl(或500 µl的大体积样本)使用特定的试剂盒和方案处理,洗脱体积为50 µl。每个实验中8个峰的峰高都已标准化。(数据由B. Bayer and K. Anslinger,Institute of Legal Medicine,Ludwig Maximilian University, Munich,Germany提供)
tip dance方式处理固体样本,操作简单

除了标准的微量方案,还可选用全自动tip dance实验方案,移液过程中滤嘴相对工作平台可来回移动。这样可在同一管中直接处理固体样本,如:拭子、组织、血斑或烟头。无需事先离心去除会阻塞滤嘴的固态物质(参见。tip dance实验方案简化了操作步骤,在某些情况下能更有效的提取样本提高DNA的产量(参见" Easy and efficient processing of solid materials with the "tip dance" protocol")。

经优化的实验方案,更大的样本容量

大体积实验方案可全自动处理的起始样本量多达500 µl。这不仅可以从含有低浓度DNA的稀释样本,如扩散的污渍中高效纯化DNA,而且能够纯化需要用大体积进行彻底裂解的样本。可处理大容量样本(与标准的微量方案有相同的洗脱体积),就可获得更高产量、更高浓度的DNA,在下游应用中有更高的灵敏度(参见" Higher yields of concentrated DNA with the large-volume protocol")。

有效纯化少量DNA

EZ1 DNA Investigator Kit使用载体RNA,用于提高效率。载体RNA可促进DNA结合到磁珠表面的硅胶上,尤其是含有少量DNA(<100 ng)的样本。公布数据表明载体RNA能够更有效的纯化法医检材样本中的少量DNA,而且对于相同的样本类型可提高DNA产量。载体RNA用于样本裂解也不会影响下游STR分析。

简单的实验设置和操作

使用预设的EZ1 Advanced XL DNA Investigator Card(用于EZ1 Advanced XL)、EZ1 Advanced DNA Investigator Card(用于EZ1 Advanced)和EZ1 DNA Investigator Card(用于BioRobot EZ1)设定纯化方案,十分便利。EZ1 DNA Investigator Kit采用预封装试剂条,减少人工操作的错误和避免缓冲液的污染。通过消除试剂准备和工作站设置时人工操作的失误,以及一个无交叉污染的液体处理,EZ1 Advanced XL、EZ1 Advanced和BioRobot EZ1可确保高度可靠性和标准化。

保证质量

依照QIAGEN's ISO-certified Quality Management System,每批EZ1 DNA Investigator Kit都经过验证,确保始终如一的产品质量。

See figures

Principle

该试剂盒配合EZ1 Advanced XL、EZ1 Advanced和BioRobot EZ1工作站,采用硅胶膜磁珠技术全自动纯化基因组DNA。磁珠技术无需重复的离心操作,每批可纯化1–14个样本(EZ1 Advanced XL)或1–6个样本(EZ1 Advanced和BioRobot EZ1)。预封装的试剂条和仪器的自动感控锁可降低污染的风险。

Procedure

将预处理样本加载到工作站,在全自动EZ1 DNA纯化操作后,DNA被一步纯化。洗脱得到的基因组DNA可直接用于各种下游应用。

Applications

使用EZ1 DNA Investigator Kit获得的高品质DNA适用于人类身份鉴定,包括:法医检材、血缘关系鉴定、亲子鉴定和受难者身份鉴定,适用如下技术:
  • STR和SNP基因分型
  • DNA定量检测
  • 线粒体或染色体DNA测序

Supporting data and figures

Specifications

FeaturesSpecifications
ApplicationsPCR, real-time RT-PCR, genotyping
Main sample typeForensic samples
For automated processingBioRobot EZ1
Sample amount<500 µl
TechnologyMagnetic particles
FormatSample tube
ProcessingAutomated
Purification of total RNA, miRNA, poly A+ mRNA, DNA or proteinDNA
Yield150–250 ng
Elution volume50–200 µl

Resources

补充实验方案 (4)
Supplementary protocol using the EZ1 Advanced DNA Investigator Large-Scale Bone Card or the EZ1 Advanced XL DNA Investigator Large-Scale Bone Card
Supplementary protocol using the EZ1 Advanced or EZ1 Advanced XL with the EZ1 DNA Investigator Kit
Supplementary protocol using the Investigator Lyse&Spin Basket Kit and EZ1 Advanced XL
Supplementary protocol using the Flip Cap Rack and the EZ1 Advanced XL Flip Cap Card.
用户开发的实验方案 (6)
This protocol has been adapted to allow the user to replace the epithelial transfer and sperm pellet wash steps with an automated protocol using the QIAcube Connect.
This protocol is adapted from the QIAGEN RNeasy MinElute Cleanup protocol and is intended for use as a guideline for the purification of total RNA from waste fractions obtained from a sample first purified using the EZ1 DNA Investigator protocol.

This protocol has been adapted by the North Louisiana Criminalistics Laboratory from the pretreatment for epithelial cells mixed with sperm cells and is for the lysis and extraction of DNA from a sperm pellet after differential separation.

This protocol has been adapted by the North Louisiana Criminalistics Laboratory from the pretreatment for hair and is for the lysis and extraction of DNA from forensic samples of hair in less time than the standard EZ1 DNA Investigator protocol pretreatment.
快速启动实验方案 (2)
For use with the EZ1 instruments
For use with the EZ2 Connect Fx instrument
验证报告 (3)
For the EZ1 DNA Investigator Kit
  • BioRobot EZ1
  • EZ1 Advanced
  • EZ1 Advanced XL
产品介绍与指南 (5)
Fully automated low- to medium-throughput purification of nucleic acids

Streamline your workflow with QIAGEN solutions for human identity and forensic testing

Solutions for missing persons and bone sample processing


New name for EZ1 DNA Investigator Kit: now EZ1&2 DNA Investigator Kit

应用说明 (5)

Performance of the EZ2 Connect Fx compared with performance of an instrument regularly encountered in forensic laboratories

Performance of the EZ2 Connect Fx compared with performance of an instrument regularly encountered in forensic laboratories

试剂盒操作手册 (2)
For automated purification of DNA from forensic and human ID samples using EZ1 instruments
For automated purification of DNA from forensic and human ID samples using the EZ2 Connect Fx instrument
安全数据表 (1)
Download Safety Data Sheets for QIAGEN product components.
技术资讯 (1)
Quality initiatives for human identity testing and forensics 
Safety Data Sheets (1)
Certificates of Analysis (1)
Application Notes (5)

Performance of the EZ2 Connect Fx compared with performance of an instrument regularly encountered in forensic laboratories

Performance of the EZ2 Connect Fx compared with performance of an instrument regularly encountered in forensic laboratories

Brochures & Guides (5)
Fully automated low- to medium-throughput purification of nucleic acids

Streamline your workflow with QIAGEN solutions for human identity and forensic testing

Solutions for missing persons and bone sample processing


New name for EZ1 DNA Investigator Kit: now EZ1&2 DNA Investigator Kit

Kit Handbooks (2)
For automated purification of DNA from forensic and human ID samples using EZ1 instruments
For automated purification of DNA from forensic and human ID samples using the EZ2 Connect Fx instrument
Validation Reports (3)
For the EZ1 DNA Investigator Kit
  • BioRobot EZ1
  • EZ1 Advanced
  • EZ1 Advanced XL
Supplementary Protocols (4)
Supplementary protocol using the EZ1 Advanced DNA Investigator Large-Scale Bone Card or the EZ1 Advanced XL DNA Investigator Large-Scale Bone Card
Supplementary protocol using the Flip Cap Rack and the EZ1 Advanced XL Flip Cap Card.
Supplementary protocol using the EZ1 Advanced or EZ1 Advanced XL with the EZ1 DNA Investigator Kit
Supplementary protocol using the Investigator Lyse&Spin Basket Kit and EZ1 Advanced XL
Quick-Start Protocols (2)
For use with the EZ1 instruments
For use with the EZ2 Connect Fx instrument
Technical Information (1)
Quality initiatives for human identity testing and forensics 
User-Developed Protocols (6)
This protocol is adapted from the QIAGEN RNeasy MinElute Cleanup protocol and is intended for use as a guideline for the purification of total RNA from waste fractions obtained from a sample first purified using the EZ1 DNA Investigator protocol.
This protocol has been adapted to allow the user to replace the epithelial transfer and sperm pellet wash steps with an automated protocol using the QIAcube Connect.
This protocol has been adapted by the North Louisiana Criminalistics Laboratory from the pretreatment for hair and is for the lysis and extraction of DNA from forensic samples of hair in less time than the standard EZ1 DNA Investigator protocol pretreatment.

This protocol has been adapted by the North Louisiana Criminalistics Laboratory from the pretreatment for epithelial cells mixed with sperm cells and is for the lysis and extraction of DNA from a sperm pellet after differential separation.

FAQ

Is training of lab personnel included with the purchase of a BioRobot EZ1?

Operation of the BioRobot EZ1 is so easy that no additional training other than the initial demo is needed. Operating the instrument requires only three major steps:

  • Insert the appropriate EZ1 Protocol Card, switch on the workstation, and, if necessary, select the appropriate protocol
  • Follow the instructions on the liquid-crystal display (LCD), which describes where the sample tubes, elution tubes, filter tips, and EZ1 Reagent Cartridges must be loaded and which indicates when to start the protocol run
  • Collect purified nucleic acids when the protocol run ends

 

FAQ ID -1239
When should the Normalization protocol be used?

The Normalization protocol is useful for routine, standardized analyses of reference samples, such as blood or buccal swabs, where sample-to-sample variability can result in widely varying DNA yields. The normalization protocol eliminates the need for yield quantification prior to analysis. It limits DNA yields uniformly to 150-250 ng.

However, please note that the Normalization protocol should not be used if the DNA amount in the sample is very limited. In this case, the Trace protocol on the EZ1 DNA Investigator Card should be used instead.

FAQ ID -1155
How can we get our BioRobot EZ1 upgraded when new protocols are launched?

Protocols for nucleic acid purification are supplied on pre-programmed EZ1 Protocol Cards. These protocols provide both on-screen instructions for the operator and operating commands for the workstation. The range of kits and protocols for the EZ1 is continuously expanding to allow nucleic acid purification from a wide range of sample types. Please browse our Supplementary Protocol Section to find specialized EZ1 protocols for a large variety of clinical sample types, in addition to the standard sample types covered in our EZ1 handbooks.

 

FAQ ID -1241
Where can I find information about the worktable setup on the BioRobot EZ1?

Loading of sample tubes, elution tubes, filter-tips, and Reagent Cartridges onto the worktable of the BioRobot EZ1 is intuitive, easy and quick. Details about worktable setup are provided with each new protocol, and are also displayed in the liquid-crystal display (LCD) of the control panel when you start setup of the worktable.

 

FAQ ID -1243
Can the EZ1 DNA Investigator Tip Dance protocol cope with any solid matrix remaining in the sample tube?
Yes, the Tip Dance protocol of the EZ1 DNA Investigator Card can cope with solid matrix that remains in the sample tube. However, very fluffy/diffuse materials (e.g. paper tissues) can clog the tips.
FAQ ID -1153
Can the EZ1 DNA Forensic Card be used with the EZ1 DNA Investigator Kit?
No, the EZ1 DNA Forensic Card cannot be used with the EZ1 DNA Investigator Kit.
FAQ ID -1151
Do the foils sealing the EZ1 Reagent Cartridges have to be manually removed prior to loading the robot?

No, prefilled Reagent Cartridges can be loaded onto the BioRobot EZ1 with their seals. During operation of the instrument, the piercing unit, a row of 6 metal spikes located behind the tip adapters, will puncture the foil of the cartridges, thereby exposing the reagents.

 

FAQ ID -1240
What disposables are required for nucleic acid isolations on the BioRobot EZ1?

No extra accessories need to be purchased for the BioRobot EZ1, as sample and elution tubes, as well as tips and tip holders are included with each EZ1 DNA or RNA isolation kit.

 

FAQ ID -1246