EZ1 DNA Investigator Card

用于在 EZ1 仪器上轻松设置法医应用的方案

S_1084_5_GEN_V2

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EZ1 DNA Forensic Card

Cat. No. / ID:  9015864

用于 BioRobot EZ1 法医方案的预编程卡
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Accessories
EZ1 DNA Card
EZ1 Advanced DNA Card
For
EZ1 DNA Tissue Kit
EZ1 DNA Investigator Kit
EZ1 卡在 EZ1 仪器上与 EZ1 试剂盒结合使用。有关产品使用的信息,请参阅特定 EZ1 试剂盒手册和 BioRobot EZ1 用户手册。 EZ1 Advanced 卡与 EZ1 试剂盒在 EZ1 Advanced 上结合使用。有关产品使用的信息,请参阅特定 EZ1 试剂盒手册和 EZ1 Advanced 用户手册。 EZ1 Advanced XL 卡在 EZ1 Advanced XL 上与 EZ1 试剂盒结合使用。有关产品使用的信息,请参阅特定 EZ1 试剂盒手册和 EZ1 Advanced XL 用户手册

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

  • 方案设置简易,如同使用信用卡一样简单
  • 标准化处理确保结果可重现
  • 经过优化的方案有助于确保纯化的可靠性
  • 通过样本跟踪实现先进的过程安全
  • 从案件样本中自动纯化基因组 DNA

Product Details

EZ1 DNA Investigator Card 预编了实验方案,用于在 EZ1 Advanced、EZ1 Advanced XL 或 BioRobot EZ1 上使用 EZ1 DNA Investigator Kit 从法医鉴定和人类身份确定样本中纯化 DNA。

Performance

EZ1 DNA Kit 与 EZ1 DNA Investigator Card 和 EZ1 仪器结合使用时可以从法医样本中快速纯化 DNA,例如:痕量样本、烟头和头发等。

通过“吸头跳动”方案更方便地处理固体材料

此外,EZ1 DNA Investigator Card 还在 EZ1 仪器上提供了可选的全自动“吸头跳动”方案,在该方案下,过滤吸头会在移液过程中相对于工作台平台来回移动。如此便可直接在样本管中处理拭子、织物、血卡或烟头等固体材料。不需要事先进行离心分离以去除可能堵塞吸头的固体材料(请参阅流程图 “EZ1 DNA Investigator 痕量和‘吸头跳动’方案”)。“吸头跳动”方案简化了处理,并且在某些情况下,可以通过更高效地提取样本来增加 DNA 产量(见图 “使用‘吸头跳动’方案轻松而又高效地处理固体材料”)。

适用于更大样本量的优化型方案

大样本量方案可实现在 EZ1 仪器上对高达 500 µl 的起始体积进行全自动处理。这不仅可以实现从具有低浓度 DNA 的稀释样本(比如分散型斑迹)中高效地纯化 DNA,还能够实现从需要增大体积以进行彻底裂解的样本中进行纯化。以与标准痕量方案相同的洗脱体积处理更大样本量的能力使得能够获得更高产量的浓缩 DNA,从而提高在下游应用中的灵敏度(请参阅 “使用大样本量方案提高浓缩 DNA 的产量”)。

See figures

Principle

EZ1 DNA Investigator Card 规定了纯化方案的选择,无需任何手动数据输入。可随时加购 EZ1 卡来扩大 EZ1 仪器的应用范围。

Procedure

只需将带有所需方案的 EZ1 卡插入卡槽并启动 EZ1 仪器。

Applications

使用插有 EZ1 DNA Investigator 卡的 EZ1 仪器获得的高品质 DNA 适用于许多应用,包括:

  • 基因身份检测和法医鉴定
  • 从痕量样本(如案件或犯罪现场样本)中纯化 DNA
  • 参考样本的常规分析
  • 多重 PCR 和实时荧光定量 PCR
  • 基因分型分析,包括 SNP、STR 或 NGS 技术

Supporting data and figures

Resources

快速启动实验方案 (2)
For use with the EZ1 instruments
For use with the EZ2 Connect Fx instrument
补充实验方案 (1)
Supplementary protocol using the EZ1 Advanced or EZ1 Advanced XL with the EZ1 DNA Investigator Kit
验证报告 (1)
For the EZ1 DNA Investigator Kit
  • BioRobot EZ1
  • EZ1 Advanced
  • EZ1 Advanced XL
试剂盒操作手册 (1)
For automated purification of DNA from forensic and human ID samples using EZ1 instruments
产品介绍与指南 (1)
Fully automated low- to medium-throughput purification of nucleic acids
Safety Data Sheets (1)
Certificates of Analysis (1)
Brochures & Guides (1)
Fully automated low- to medium-throughput purification of nucleic acids
Kit Handbooks (1)
For automated purification of DNA from forensic and human ID samples using EZ1 instruments
Validation Reports (1)
For the EZ1 DNA Investigator Kit
  • BioRobot EZ1
  • EZ1 Advanced
  • EZ1 Advanced XL
Supplementary Protocols (1)
Supplementary protocol using the EZ1 Advanced or EZ1 Advanced XL with the EZ1 DNA Investigator Kit
Quick-Start Protocols (2)
For use with the EZ1 instruments
For use with the EZ2 Connect Fx instrument

FAQ

Is training of lab personnel included with the purchase of a BioRobot EZ1?

Operation of the BioRobot EZ1 is so easy that no additional training other than the initial demo is needed. Operating the instrument requires only three major steps:

  • Insert the appropriate EZ1 Protocol Card, switch on the workstation, and, if necessary, select the appropriate protocol
  • Follow the instructions on the liquid-crystal display (LCD), which describes where the sample tubes, elution tubes, filter tips, and EZ1 Reagent Cartridges must be loaded and which indicates when to start the protocol run
  • Collect purified nucleic acids when the protocol run ends

 

FAQ ID -1239
What EZ1 DNA Investigator protocol is recommended for very precious samples?
For precious samples, please use the Trace Protocol of the EZ1 DNA Investigator Card on the BioRobot EZ1.
FAQ ID -1154
When should the Normalization protocol be used?

The Normalization protocol is useful for routine, standardized analyses of reference samples, such as blood or buccal swabs, where sample-to-sample variability can result in widely varying DNA yields. The normalization protocol eliminates the need for yield quantification prior to analysis. It limits DNA yields uniformly to 150-250 ng.

However, please note that the Normalization protocol should not be used if the DNA amount in the sample is very limited. In this case, the Trace protocol on the EZ1 DNA Investigator Card should be used instead.

FAQ ID -1155
How can we get our BioRobot EZ1 upgraded when new protocols are launched?

Protocols for nucleic acid purification are supplied on pre-programmed EZ1 Protocol Cards. These protocols provide both on-screen instructions for the operator and operating commands for the workstation. The range of kits and protocols for the EZ1 is continuously expanding to allow nucleic acid purification from a wide range of sample types. Please browse our Supplementary Protocol Section to find specialized EZ1 protocols for a large variety of clinical sample types, in addition to the standard sample types covered in our EZ1 handbooks.

 

FAQ ID -1241
Where can I find information about the worktable setup on the BioRobot EZ1?

Loading of sample tubes, elution tubes, filter-tips, and Reagent Cartridges onto the worktable of the BioRobot EZ1 is intuitive, easy and quick. Details about worktable setup are provided with each new protocol, and are also displayed in the liquid-crystal display (LCD) of the control panel when you start setup of the worktable.

 

FAQ ID -1243
Can the EZ1 DNA Investigator Tip Dance protocol cope with any solid matrix remaining in the sample tube?
Yes, the Tip Dance protocol of the EZ1 DNA Investigator Card can cope with solid matrix that remains in the sample tube. However, very fluffy/diffuse materials (e.g. paper tissues) can clog the tips.
FAQ ID -1153
Can the EZ1 DNA Forensic Card be used with the EZ1 DNA Investigator Kit?
No, the EZ1 DNA Forensic Card cannot be used with the EZ1 DNA Investigator Kit.
FAQ ID -1151
How to store DNA from forensic samples eluted into water on the EZ1 biorobot in TE buffer instead?

For storage of DNA purified from forensic samples using the EZ1 DNA Tissue Kit in a buffered solution such as TE rather than water (used for elution in the EZ1 forensic protocols) we suggest the following:

Before storing the DNA, add 1/10 volume of 10x Forensic TE buffer (100 mM Tris·Cl, 1 mM EDTA, pH 8.3) to each sample. For example, add 5 µl of 10x Forensic TE buffer if you eluted in 50 µl. The DNA is ready to use, or can be stored at 2–8°C for 24 h, or at –20°C for longer periods.

FAQ ID -734
Do the foils sealing the EZ1 Reagent Cartridges have to be manually removed prior to loading the robot?

No, prefilled Reagent Cartridges can be loaded onto the BioRobot EZ1 with their seals. During operation of the instrument, the piercing unit, a row of 6 metal spikes located behind the tip adapters, will puncture the foil of the cartridges, thereby exposing the reagents.

 

FAQ ID -1240
How do I safely inactivate biohazardous flow-through material?

Always dispose of potentially biohazardous solutions according to your institution’s waste-disposal guidelines. Although the lysis and binding buffers in QIAamp, DNeasy, and RNeasy kits contain chaotropic agents that can inactivate some biohazardous material, local regulations dictate the proper way to dispose of biohazards. DO NOT add bleach or acidic solutions directly to the sample-preparation waste. Guanidine hydrochloride in the sample-preparation waste can form highly reactive compounds when combined with bleach.
Please access our Material Safety Data Sheets (MSDS) online for detailed information on the reagents for each respective kit.

FAQ ID -12
What disposables are required for nucleic acid isolations on the BioRobot EZ1?

No extra accessories need to be purchased for the BioRobot EZ1, as sample and elution tubes, as well as tips and tip holders are included with each EZ1 DNA or RNA isolation kit.

 

FAQ ID -1246