PAXgene Bone Marrow RNA Tubes

用于收集骨髓样本,并稳定/纯化细胞内RNA

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PAXgene Bone Marrow RNA Tubes (50)

Cat. No. / ID:  764114

50 Bone Marrow Collection Tubes. To be used in conjunction with the PAXgene Bone Marrow RNA Kit
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仅作研究用途。不用于诊断程序。任何表述或表示都并非旨在提供诊断、预防或治疗疾病的信息。
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Features

  • 完整的体系,用于采集、稳定和纯化
  • 收集时立即稳定胞内RNA
  • 从骨髓中快速纯化RNA
  • 安全方便的运输

Product Details

PAXgene Bone Marrow RNA System包含PAXgene Bone Marrow RNA Tube收集管和PAXgene Bone Marrow RNA Kit纯化试剂盒,前者用于稳定、储存和运输骨髓样本,后者应用硅胶膜和离心柱技术对RNA进行分离纯化。骨髓样本可以用标准的医学方法收集并加入常用的抗凝剂。每份2 ml的骨髓样本立即存入一支PAXgene Bone Marrow RNA Tube中,该收集管可以立即稳定样本中的RNA,并适合在室温、冷藏或冷冻条件下保存。样本可在几天后使用PAXgene Bone Marrow RNA Kit进行纯化,也可在–20°C或–70°C条件下保存数月。

Performance

PAXgene Bone Marrow RNA Kit可从不同的人类骨髓样本中高效分离和纯化细胞内RNA(参见" RNA yields from bone marrow samples"),包括多细胞、黏性样本的优化实验方案。 样品可在室温、冷藏或冷冻条件下保存几天后使用PAXgene Bone Marrow RNA Kit进行纯化,获得完整的高品质RNA(参见" RNA stability at room temperature"、" RNA stabilized at 2–8°C"和" High RNA integrity after freeze–thaw cycles")。RNA Tubes可防止RNA分子被RNases降解,并减少体外基因表达的变化,确保准确进行分析(参见" Stability of 5 transcripts at room temperature")。试剂盒自带DNase 处理,确保基因组DNA残留量达到最小。内部和外部研究均表明,95% 的检测样本(n=157)中的基因组DNA含量小于0.85% 。A260/A280值一般在1.8–2.2之间,表明产物为高品质RNA(参见" Storage under different conditions")。
See figures

Principle

在室温下储存或运输骨髓样本时,骨髓中每种mRNA的拷贝数会有巨大变化,给基因表达研究带来了巨大挑战。PAXgene Bone Marrow RNA Tubes可防止RNA分子被RNases降解,并适合于在室温、冷藏或冷冻条件下保存。

Procedure

骨髓样本可以用标准的医学方法收集并加入常用的抗凝剂。每份2 ml的骨髓样本立即存入一支PAXgene Bone Marrow RNA Tube中,该收集管可以立即稳定样本中的RNA ,并适合于在室温、冷藏或冷冻条件下保存。PAXgene Bone Marrow RNA Kit可方便、快速的从人类骨髓样本中分离并纯化细胞内RNA 。该试剂盒使用离心柱,采用硅胶膜技术,可高效纯化完整的高品质RNA 。

Applications

使用PAXgene Bone Marrow RNA Kit纯化的RNA可即用于各种下游应用,包括:

  • Real-time RT-PCR分析特定的转录产物
  • 复杂的表达谱芯片分析

Supporting data and figures

Specifications

FeaturesSpecifications
Tube size16 x 125 mm
Label typePaper
Draw volume2 ml
Quantity50/box
Additive/concentrationAdditive 11.1 ml
Closure type/colorBD Hemogard/Red
Stabilizationup to 3 days at room temperature (15–25°C); up to 5 days at 2–8°C; long-term at –20°C or –70°C

Resources

产品介绍与指南 (3)
For collection, storage, and transport of bone marrow samples and stabilization and purification of intracellular RNA

 

安全数据表 (1)
Download Safety Data Sheets for QIAGEN product components.
Technical Information and Important Notes (2)
This note is to inform you that the street address for PreAnalytiX GmbH has changed from “Feldbachstrasse” to “Garstligweg 8”. Please be informed that the update of the product labeling to the new address is ongoing.
In order to reduce paper consumption and oblige the growing number of customers requesting an environmentally friendly alternative to traditionally printed handbooks, we are now providing kit handbooks for Research Use Only (RUO) PreAnalytiX kits on our website only.
试剂盒操作手册 (2)
For the collection of human bone marrow and immediate stabilization of intracellular RNA
For isolation and purification of intracellular RNA from whole bone
marrow samples stabilized in PAXgene Bone Marrow RNA Tubes
Safety Data Sheets (1)
Certificates of Analysis (1)
Brochures & Guides (2)
For collection, storage, and transport of bone marrow samples and stabilization and purification of intracellular RNA

 

Gene Expression Analysis (1)
Kit Handbooks (2)
For isolation and purification of intracellular RNA from whole bone
marrow samples stabilized in PAXgene Bone Marrow RNA Tubes
For the collection of human bone marrow and immediate stabilization of intracellular RNA

FAQ

Where can I find additional information for PreAnalytiX PAXgene products?
You can find additional information relating to the PreAnalytiX PAXgene products on the PreAnalytiX website .
FAQ ID - 3515
How much time may the aspirated bone marrow sample be allowed to incubate before pipetting it into the PAXgene Bone Marrow RNA Tube?
As gene expression starts to change in a sample removed from the human body within minutes, we highly recommend transferring the samples within a few minutes into the stabilization solution of the PAXgene Bone Marrow RNA tubes for cell lysis directly linked to RNA stabilization to take place.
FAQ ID -2504
I routinely heat an aliquot of the RNA eluate prior to my RT reaction. Do I still need to heat the eluate after the PAXgene Bone Marrow RNA preparation?
Yes. Heating temperature and incubation time will vary depending on different RT protocols. Therefore, the final heating step should be performed routinely at the end of the protocol.
FAQ ID -2513
Can I use water to elute the RNA in the PAXgene Bone Marrow RNA Kit?
No. The elution buffer (Buffer BMR5) supplied in the PAXgene Bone Marrow RNA Kit must be used.
FAQ ID -2512
Can specific types of white blood cells be enriched from a PAXgene Bone Marrow RNA Tube prior to the RNA isolation?
No. The cells are lysed in the PAXgene Bone Marrow RNA Tube. There is no method available to enrich for certain sub-populations of cells.
FAQ ID -2508
How are the supernatants resulting from the first two spin steps of the PAXgene Bone Marrow RNA kit purification procedure disposed?

The supernatants resulting from the first two spin steps of the PAXgene Bone Marrow RNA Kit purification procedure may still contain biologically hazardous material. We recommend classifying the tube-processed supernatant of the kit as both a biological and chemical waste. Supernatants can be decontaminated by autoclaving and disposed of as chemical waste.  Disposal should be done in accordance with local, state and federal regulations.

CAUTION: Do not add bleach or acidic solutions directly to the sample preparation waste.

FAQ ID -2516
Can the PAXgene Bone Marrow RNA System be used with animal bone marrow or with blood samples?
The PAXgene Bone Marrow RNA System was developed for intracellular RNA isolation from human bone marrow samples. Bone marrow samples from other species or from blood were not tested.
FAQ ID -2501
Can bone marrow from leukemia patients with elevated numbers of white blood cells be processed with the PAXgene Bone Marrow RNA System?
Yes. We have tested the PAXgene Bone Marrow RNA System with bone marrow samples containing very high numbers of leukocytes. Bone marrow samples with higher leukocyte numbers are indicated by a highly viscous solution after addition of buffers BMR1 and BMR2 followed by incubation. These challenging samples are further diluted according to descriptions given in the protocol and do not lead to problems in the extraction procedure.
FAQ ID -2510
Can the PAXgene RNA stabilization solution/bone marrow mixture from the PAXgene Bone Marrow RNA Tube be disposed of by adding bleach?
Yes. You may dispose of the waste product from the PAXgene Bone Marrow RNA Tube with a 5% sodium hypochlorite bleach solution, in a 1:9 ratio (1 part bleach : 9 parts tube contents).
FAQ ID -2515
How should the PAXgene Bone Marrow RNA Tubes be frozen after collection and for how long?

The PAXgene Bone Marrow RNA Tubes should be frozen at –20°C and –70°C in a wire rack, not in an expanded polystyrene foam tray.

If the PAXgene Bone Marrow RNA Tubes are to be stored at temperatures lower than –20ºC, freeze them first for 24 h at –20ºC, then transfer them to –70ºC.

Currently available data shows stabilization of cellular RNA for several months at –20°C or –70°C. For more information from ongoing studies evaluating stability for longer time periods, please contact QIAGEN Technical Services.

 

 

FAQ ID -2505
What RNA yields are expected with the PAXgene Bone Marrow RNA System?

Bone marrow samples are extremely heterogeneous, consisting of varying amounts of cells, tissue, and solid matter. RNA yields are highly donor-dependent and can vary greatly from sample to sample as well as between replicates from the same donor due to sample-intrinsic heterogeneity.

In a study of 295 bone marrow samples, yields of RNA isolated from 2 mL human bone marrow were between 2.0 and 44.7 µg (5-95% quantiles, mean = 15.8 µg, median = 11.0 µg).

FAQ ID -2506
Is RNA from red blood cells also isolated when RNA is extracted from a PAXgene Bone Marrow RNA Tube?
Yes. The PAXgene Bone Marrow RNA System purifies RNA from whole bone marrow. Therefore it will also purify RNA from other cells, e.g. reticulocytes and RBCs. Array analyses have shown higher globin mRNA levels for RNA isolated from whole bone marrow compared to RNA isolated from mononuclear cells.
FAQ ID -2509
Is the 260/230 nm absorbance ratio specified for RNA derived from the PAXgene Bone Marrow RNA System?

No. The 260/230 nm absorbance ratio is not specified for the PAXgene Bone Marrow RNA extraction kit. In contrast to a reduced 260/280 ratio, which results from a protein contamination of the eluted RNA and can lead to inhibitory effects in down stream applications, there is no negative impact of a reduced 260/230 ratio on any down stream application reported to date and therefore it is not necessary to specify this.

In addition, because the elution buffer contains very low concentrations of salt that absorb at wavelengths between 220 and 230 nm, it is necessary to zero the photometer properly. Do this by preparing a solution for zeroing the photometer which contains a similar salt concentration as the sample to be measured by using the same volumes. Add the same volume of Buffer BMR5 as the volume of RNA sample to be diluted into a fresh volume of the buffer in which you will measure the absorption (we recommend 10 mM Tris Cl pH 7.5 buffer for this purpose).

FAQ ID -2514
What is in the elution buffer in the PAXgene Bone Marrow RNA kit?
The PAXgene Bone Marrow RNA kit elution buffer (Buffer BMR5) is a proprietary low-salt buffer. In combination with the final heat denaturation step, it leads to optimal performance of the isolated RNA in RT-reactions. There is no interference with downstream applications reported to date.
FAQ ID -2511
Can the PAXgene Bone Marrow RNA System be used for other human body fluids than bone marrow samples or cultured cells of human origin?
No. The PAXgene Bone Marrow RNA System was developed for intracellular RNA isolation from human bone marrow samples. PreAnalytiX offers a dedicated collection and RNA extraction system for human blood (see the PAXgene Blood RNA System). QIAGEN also offers RNA stabilization and isolation systems for saliva and cultured cells. Please contact QIAGEN technical service for more information or visit www.qiagen.com.
FAQ ID -2502
Can bone marrow aliquots other than 2 mL be pipetted into the PAXgene Bone Marrow RNA Tube?
No. Transferring bone marrow volumes different than 2mL into the PAXgene Bone Marrow RNA Tube is not recommended. The tube and its contents were optimized for performance with 2 mL of bone marrow. The use of other volumes is not recommended.
FAQ ID -2503
Can the PAXgene Bone Marrow RNA System be used to isolate genomic DNA or viral RNA and DNA?
No. The PAXgene Bone Marrow RNA System has been optimized only for cellular RNA only.
FAQ ID -2507