miScript miRNA Mimic

适合利用合成 miRNA 研究 miRNA 功能和基因调控

S_1084_5_GEN_V2
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miScript miRNA Mimic

Cat. No. / ID:  219600

1 nmol(细胞培养级)、5 nmol(细胞培养级)或 20 nmol(动物级)miScript miRNA Mimic,管内提供
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Configure at GeneGlobe
Find or custom design the right target-specific assays and panels to research your biological targets of interest.

Features

  • 即转染模拟物的行为与内源性 miRNA 相似
  • 可用于 miRBase 中的所有人类、小鼠和大鼠 miRNA
  • 高度灵活的格式、规模和等级
  • 可通过 GeneGlobe 轻松订购

Product Details

miScript miRNA Mimic 是化学合成的双链 RNA,转染细胞后可模拟成熟的内源性 miRNA。miScript miRNA Mimic 可用于细胞培养级(纯度大于 90%)或动物级(HPLC 纯化;用于体内应用)。对于灵活的筛选,miScript miRNA Mimic Plate 使研究人员能够订购任何预先设计的 miScript miRNA Mimic,并选择 96 孔板或 384 孔板的布局。对于转染,QIAGEN 提供用于 miRNA Mimic 转染的 HiPerFect 转染试剂和用于 miRNA Mimic-DNA 共转染的 Attractene 转染试剂。

要想在 miRNA 研究中获得更高的性能,不妨试试我们的 miRCURY LNA miRNA Mimic。这些高效、成熟的 miRNA 模拟物采用独特的三重 RNA 链设计,可获得更好的结果。

Performance

miScript miRNA Mimic 导致的基因表达下降与内源性 miRNA 的表达下降相似(见图“ 内源性 miRNA 和 miScript miRNA 拟定物的相似下调作用”)。观察到的基因表达下降通常是在平移水平上引起的(见图“ miR-1 Mimic 转染后蛋白质水平的下调”)。miScript miRNA Mimic 可在转染后 72 小时内有效抑制基因表达(见图“ miScript miRNA Mimic 可在 72 小时内保持稳定”)。
See figures

Principle

转染模拟物后进行下游基因表达分析或表型分析,以阐明特定 miRNA 的靶标和作用。通过这些实验,可以研究单个 miRNA 失调的生物学效应,并确认特定基因是单个 miRNA 的靶标。转染模拟物后基因表达的减少证明所研究的 miRNA 参与了该基因的调控。miScript miRNA 抑制剂也可用于类似的研究。一般来说,这类实验包括转染模拟物或抑制剂,或者与携带 miRNA 结合位点的载体构建体融合转染报告基因。下游分析可包括报告基因检测、real-time PCR、微阵列分析或蛋白质分析。

Procedure

miScript miRNA Mimic 可用于当前版本的 microRNA 数据库 miRBase 中的所有人类、小鼠、大鼠和病毒 miRNA。QIAGEN 的 GeneGlobe 数据库不断更新,以确保模拟物和抑制剂的设计与最新版本的 miRBase 匹配。这可确保您始终使用最准确、最新的 miRNA 序列信息。

灵活的格式可实现少量模拟物的低通量分析,以及数百种模拟物的高通量筛选。miScript miRNA Mimic 可提供细胞培养级(通过专有合成和纯化工艺提供大于 90% 的纯度)的试管(1 nmol 或 5 nmol)。对于体内应用,可订购 HPLC 纯化的动物级 miScript miRNA Mimic,规格为 20 nmol(以管装形式提供)。对于中高通量应用,可提供 96 孔板(1 nmol 或 5 nmol)或 384 孔板(1 nmol),以满足您的实验要求。

Applications

  • miRNA 研究
  • 基因调控研究

Supporting data and figures

Specifications

FeaturesSpecifications
Design可提供预先设计和定制设计
Species人、小鼠、大鼠
Format单管、96 孔板和 384 孔板
Scale1 nmol、5 nmol、20 nmol
Modification
Sequence provided

Resources

安全数据表 (1)
Download Safety Data Sheets for QIAGEN product components.
产品介绍与指南 (2)
Simultaneously profile mRNA, miRNA and lncRNA using a simple, complete workflow
Safety Data Sheets (1)
Certificates of Analysis (1)

FAQ

What is the turn around time for custom miScript miRNA mimics, inhibitors and primer assays?

The turn around time for custom miScript miRNA Mimics, Inhibitors and Primer Assays is 2-3 business days, plus 2 days for shipping.

 

FAQ ID -1985
What kind of molecules are miScript miRNA Mimics?

miScript miRNA Mimics are synthetic double stranded, blunt end, RNA oligos.

 

FAQ ID -1986
Where can I find QIAGEN products for a specific gene or gene product?
You can search for specific gene products in the QIAGEN GeneGlobe Database. This easy-to-use, comprehensive Web portal allows you to find information about, search for, and order high-quality products for human, mouse, and rat genes. QIAGEN provides a vast range of gene-specific products covering every aspect of an experiment, from gene silencing to expression analysis at the mRNA or protein level.
FAQ ID -803
Does HiPerFect work for the transfection of miScript miRNA mimics and inhibitors?

HiPerFect Transfection Reagent does work for the transfection of miScript miRNA Mimics and Inhibitors, and the new handbook contains protocols for this application.

 

 

FAQ ID -1984
What is the "seed sequence" of a miRNA?
miRNAs regulate the gene expression by binding to the mRNA. The seed sequence is essential for the binding of the miRNA to the mRNA. The seed sequence or seed region is a conserved heptametrical sequence which is mostly situated at positions 2-7 from the miRNA 5´-end. Even though base pairing of miRNA and its target mRNA does not match perfect, the “seed sequence” has to be perfectly complementary.
FAQ ID -2262