QIAzol Lysis Reagent

在纯化RNA前,有效裂解脂肪和标准组织样本

S_1281_GEF_RNALT0248

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QIAzol Lysis Reagent (200ml)

Cat. No. / ID:  79306

200 ml QIAzol Lysis Reagent
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QIAzol Lysis Reagent 旨在用于分子生物学应用。该产品不能用于疾病诊断、预防和治疗。

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

  • 优化的裂解条件,纯化得到可用于基因表达分析的RNA
  • 从脂肪组织中得到高产量RNA
  • 易于操作的裂解和匀质化流程
  • 与RNeasy纯化试剂配合使用,避免苯酚污染
  • 适用于多种类型组织样本

Product Details

QIAzol Lysis Reagent适合裂解脂肪组织。标准的QIAzol实验方案提供易于操作的实验流程,整合了QIAzol裂解、标准匀质化方法和异丙醇沉淀。

Performance

使用QIAzol对组织样本进行有机提取,可有效去除基因组DNA,提高提取的RNA在下游应用中的效率。QIAzol适合裂解脂肪组织,与RNeasy硅胶膜技术相结合,可从脂肪组织中得到高品质RNA(参见" High-quality RNA")。
See figures

Principle

基于苯酚/胍的QIAzol Lysis Reagent适用于多种类型的组织,特别适合裂解脑和脂肪等多脂组织。有机提取和离液剂作用相结合可高效裂解组织,得到高产量的总RNA。有机提取步骤可去除蛋白质和DNA,提高后续纯化步骤的效率,如沉淀或离心。QIAGEN提供有效纯化RNA的多种方案,在单个、简化的操作流程中整合了QIAzol裂解技术和RNeasy硅胶膜纯化技术(如使用RNeasy Plus Universal Kits处理多种类型组织)。

Procedure

标准的QIAzol实验方案提供易于操作的实验流程,整合了QIAzol裂解、标准匀质化方法和异丙醇沉淀。组织样本在QIAzol Lysis Reagent中匀质化。加入氯仿后离心,匀浆分为水相和有机相。RNA位于上层水相,DNA位于分界面,蛋白质位于下层的有机相。加入异丙醇使RNA沉淀。然后使用乙醇洗涤沉淀,将其在RNase-free水中再次溶解。为在下游应用中有更高的性能,在后续的RNA纯化过程中,推荐使用RNeasy MinElute Cleanup Kit去除苯酚污染,浓缩RNA。

Applications

基于苯酚/胍的QIAzol Lysis Reagent适合多种组织,特别适合脑、脂肪等多脂组织。我们推荐使RNeasy Plus Universal Kit或RNeasy MinElute Cleanup Kit纯化RNA,得到的RNA在多种下游应用中表现良好。

Supporting data and figures

Resources

安全数据表 (1)
Download Safety Data Sheets for QIAGEN product components.
试剂盒操作手册 (1)
QIAzol Handbook
PDF (986KB)
For efficient lysis of fatty tissues and all other types of tissue before RNA purification
快速启动实验方案 (1)
Safety Data Sheets (1)
Certificates of Analysis (1)
Kit Handbooks (1)
QIAzol Handbook
PDF (986KB)
For efficient lysis of fatty tissues and all other types of tissue before RNA purification
Quick-Start Protocols (1)

Publications

Visceral adiposity and endothelial lipase.
Paradis ME; Badellino KO; Rader DJ; Tchernof A; Richard C; Luu-The V; Deshaies Y; Bergeron J; Archer WR; Couture P; Bergeron N; Lamarche B;
J Clin Endocrinol Metab; 2006; 91 (9):3538-43 2006 Jun 13 PMID:16772345
Targeted gene silencing in the model mushroom Coprinopsis cinerea (Coprinus cinereus) by expression of homologous hairpin RNAs.
Wälti MA; Villalba C; Buser RM; Grünler A; Aebi M; Künzler M;
Eukaryot Cell; 2006; 5 (4):732-44 2006 Apr PMID:16607020
A comprehensive description of the transcriptome of the hypothalamoneurohypophyseal system in euhydrated and dehydrated rats.
Hindmarch C; Yao S; Beighton G; Paton J; Murphy D;
Proc Natl Acad Sci U S A; 2006; 103 (5):1609-14 2006 Jan 23 PMID:16432224

FAQ

What is the composition of QIAzol? What is the color of this reagent?
For proprietary reasons, the exact composition of QIAzol is confidential.  However, it does contain phenol and thiocyanate compounds.  It is pink in color.
FAQ ID - 3355
When using QIAzol Lysis Reagent for RNA extraction, I see a pinkish colored aqueous phase! Is it OK?
Yes. Depending on the starting material the aqueous phase might be colored. It will not harm the RNA extraction procedure. It may be a hint at overloading.
FAQ ID -534
Do you have a protocol for the isolation of RNA from bacterial cultures using RNAprotect Bacteria Reagent and QIAzol Lysis Reagent?
Yes, please follow the protocol in the RNAprotect Bacteria Reagent Handbook in Appendix D:  Stabilization and Purification of Bacterial RNA Using RNAprotect Bacteria Reagent, the RNeasy Lipid Tissue Mini Kit, and the TissueLyser.
FAQ ID -1020
How pure should the chloroform used with the RNeasy Plus Universal Mini Kit be?
In general, we recommend using Molecular Biology or higher grade chloroform, which should not contain isoamyl alcohol.
FAQ ID -2344
Do you have a protocol for the isolation of genomic DNA and/or proteins from fatty tissue treated with QIAzol?
FAQ ID -955
Do you have a protocol for purification of total RNA from fatty tissues using QIAzol Lysis Reagent and MaXtract High Density?

Yes, we have the following protocols:

  • Purification of total RNA from fatty tissues using QIAzol Lysis Reagent, MaXtract High Density, and the TissueRuptor (RY29).
  • Purification of total RNA from fatty tissues using QIAzol Lysis Reagent, MaXtract High Density, and the TissueLyser (RY30).
  • Purification of total RNA from fatty tissues using the RNeasy Lipid Tissue Mini Kit and MaXtract High Density (RY31).
FAQ ID -1550
What is the composition of Buffer RWT?
The exact composition of Buffer RWT is confidential. Buffer RWT is a proprietary component of, for example, the miRNeasy Mini Kit and the RNeasy Plus Universal Kit. Guanidine salt and ethanol are important ingredients in Buffer RWT. Ethanol is added by the user prior to the first use of the kit. Buffer RWT is a stringent washing buffer used after preclearing the sample with QIAzol Lysis Reagent, especially if isolation of small RNAs, for example, microRNAs or RNAs from formalin-fixed tissue, is desired
FAQ ID -2798
Which chloroform should I use for the RNeasy Lipid Tissue Kit?
In general we recommend to use Molecular Biology or higher grade chloroform.
FAQ ID -516
How is the RNeasy Lipid Tissue Mini Kit different from the RNeasy Mini Kit?
The RNeasy Lipid Tissue Kits have been designed for the optimal lysis of tissues rich in fat, such as brain and adipose tissues. Lysis and homogenization is performed using QIAzol, a phenol/guanidine-based lysis reagent. The RNA is then purified from the aqueous fraction using a RNeasy spin column. The RNeasy Lipid Tissue Kit combines optimal lysis of fatty tissues with silica membrane technology, resulting in high-quality total RNA.
FAQ ID -468
Can I homogenize samples in QIAzol using the QIAshredder?
Yes
FAQ ID -501
Is it possible to use a QIAzol lysate for the isolation of RNA from tissue on the BioRobot EZ1?
Yes, we recommend using the EZ1 RNA Universal Tissue Kit, containing the QIAzol Lysis Reagent, for purification of total RNA from any type of animal and human tissue on the BioRobot EZ1.
FAQ ID -573