Cignal Reporter Controls

用于Cignal Reporter Assays的对照实验

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Cat. No. / ID: 336881

Cignal Reporter Control

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Cignal Reporter Controls 旨在用于分子生物学应用。这些产品不能用于疾病诊断、预防和治疗。

Configure at GeneGlobe

Find or custom design the right target-specific assays and panels to research your biological targets of interest.

Features

非常适用于优化转染条件
确立多种处理效果的特异性
可即用于转染
高灵敏度和特异性

Product Details

Cignal Reporter Assays通过检测下游转录因子的活性，对信号转导通路的活性进行快速、灵敏的定量检测。当使用Cignal Reporter Assays进行实验时，合适的对照是实验设计的关键因素。它们能准确解释报告基因的检测结果，并确保反应具有特异性。

Principle

Cignal Reporter Controls是预制的转染即用型载体。双荧光素酶和GFP报告基因都有阳性和阴性对照。

以下对照可用。

可用的对照
对照	描述
阳性对照（GFP）	持续表达GFP基因。含有绿色荧光蛋白，可简单确定转导效率和优化转导条件
阴性对照（GFP）	不可诱导、无启动子驱动GFP基因。确立多种处理效果的特异性和GFP荧光背景
阳性对照（luc）	40:1:1混合持续表达的GFP、萤火虫荧光素酶和海肾荧光素酶载体。确定转导效率，并作为萤火虫荧光素酶分析的阳性对照
阴性对照（luc）	40:1混合不诱导萤火虫荧光素酶载体和持续表达海肾荧光素酶的载体。确立多种处理效果的特异性

Procedure

Cignal Lenti Reporter Control Assays是预滴定的转导即用型慢病毒颗粒，用于优化转导条件。使用标准化流程转导Cignal Lenti Reporter Control Assays进入选定的细胞，将转导了对照的细胞与转导了报告基因的细胞采用相同方式处理。

Applications

使用Cignal Reporter Assays进行实验时，Cignal Reporter Controls是实验设计的关键因素。其适用于：

优化多种细胞的转染条件

定量检测转染效率

确立多种处理效果的特异性

确定背景GFP荧光或荧光素酶的活性

作为标准化的内参

Supporting data and figures

Cignal Reporter Controls.

Resources

Search for transfection data by nucleic acid, cell line, and transfection reagent. Our database contains data from researchers like yourself who have shared their experimental results with us.

Transfection protocols for specific cell types and plate formats that save you the time and effort of adapting existing protocols to fit your requirements. Simply select the cell type, nucleic acid, and culture format to receive a QIAGEN transfection protocol to print out or download in convenient PDF format.

Download Safety Data Sheets for QIAGEN product components.

For cell-based pathway activity assays

For cell-based analysis of pathway signaling activity

FAQ

Any transfection reagent that yields 25-30% transfection efficiency in the cell line of interest is suitable for use with DNA-based Cignal Reporter Assays.

FAQ ID -2764

No, The Cignal Reporter Assays do not currently support this functionality.

FAQ ID -2765

No, DNA-based Cignal Reporter Assays are not designed for bacterial transformations.

FAQ ID -2762

No, the positive control only serves as a constitutively-active reporter enabling the user to assess if the transfection/transduction was successful, and should not be used for direct comparison to the pathway reporters.

FAQ ID -2766

MOI is an abbreviation for Multiplicity of Infection or the number of viral particles exposed to a cell.

FAQ ID -2768

This has to be empirically determined by the user. By using a positive control, Cignal Lenti Reporter Control, the user can set up parallel transductions with increasing amounts of virus to identify an MOI that yields a robust signal.

FAQ ID -2770

No, The negative control only serves as a non-inducible reporter, but should not be used for direct comparison to the pathway reporters.

FAQ ID -2767

No, the DNA-based Cignal Reporter Assays are not designed to generate stable pathway sensor cell lines. However, the Cignal Lenti Reporter Assays can be used to generate stable pathway sensor cell lines.

FAQ ID -2763

The concentration of SureENTRY Transduction Reagent is 10 mg/ml.

FAQ ID - 3708

The DNA-based Reporters are designed for cells that are amenable to transfection. QIAGEN recommends a minimum transfection efficiency of 25 – 30%. If the cells are refractory to transfection then the Cignal Lenti reporters should be selected.

FAQ ID -2761

This depends on the Multiplicity of Infection (MOI) required for a specific cell line. The lower the MOI the more cells that can be transduced. Therefore, to predict how much Cignal Lenti Reporter is required an MOI must be determined.

FAQ ID -2769