QIAamp DSP DNA Blood Mini Kit

从人类全血中纯化基因组DNA

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QIAamp DSP DNA Blood Mini Kit

Cat. No. / ID:  61104

For 50 preps: QIAamp Mini Spin Columns, Buffers, Reagents, Tubes, VacConnectors
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QIAamp DSP DNA Blood Mini Kit 旨在用于体外诊断用途。
QIAamp DSP DNA Blood Mini Kit 旨在用于体外诊断用途。

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

  • 通用的DNA纯化系统
  • 灵活的样品采集
  • 与各种抗凝剂和试管兼容
  • 可选离心或真空处理方案

Product Details

QIAamp DSP DNA Blood Mini Kit采用硅胶模技术纯化DNA,适用于进行血液DNA纯化的实验室。

Performance

使用QIAamp DSP DNA Blood Mini Kit纯化的基因组DNA可即用于基于酶扩增或其他修饰的各种下游应用,如PCR。

全血样品使用装有抗凝剂(EDTA或柠檬酸盐)的不同类型的管子收集,包括:BD Vacutainer、Monovette和Vacuette管(参见下表)。血液样品可反复冻融至少3次,仍可用于DNA纯化(参见" DNA from frozen and thawed blood")。

使用不同的管子收集血液
            管子        生产商 编号 体积 200 µl的平均产量
BD Vacutainer 9NC BD 366007    9 ml 6.4 µg
BD Vacutainer K3E BD 368457  10 ml 6.6 µg
BD Vacutainer K2E BD 367864    6 ml 6.4 µg
S-Monovette EDTA Sarstedt 02.1066.001    9 ml 6.5 µg
S-Monovette CPDA1 Sarstedt 01.1610.001 8.5 ml 6.3 µg
Vacuette K3E Greiner Bio-One 455036    9 ml 6.5 µg
Vacuette 9NC Greiner Bio-One 454382    2 ml 6.3 µg
每种类型的管子收集11个志愿者的血液样本。每个样本使用QIAamp DSP DNA Blood Mini Kit从200 μl血液中纯化DNA,然后洗脱到200 µl洗脱缓冲液中,每个样本重复三次。该表格获得的是33次纯化的DNA的平均产量。
See figures

Principle

QIAamp DSP DNA Blood Mini Kit采用成熟的QIAamp技术纯化基因组DNA。QIAamp硅胶膜特异性结合裂解样品中的DNA,其他裂解物通过离心或真空操作去除。经过有效地洗涤去除污染物,然后洗脱DNA,洗脱体积为50–200 µl。

Procedure

有两个可供选择的流程用于从血液中纯化基因组DNA,使用离心机或使用真空抽滤装置和离心机(参见" Procedure")。

含有EDTA或柠檬酸盐的血液样品(200 µl)用含QIAGEN Protease的裂解缓冲液56℃裂解10分钟。乙醇加入到裂解物中,使DNA特异性结合到QIAamp硅胶膜上。裂解物上样到QIAamp Mini离心柱,离心或真空处理。DNA特异性结合到QIAamp硅胶膜上,污染物流走。结合的DNA使用两种不同的缓冲液有效洗涤,离心干缩QIAamp硅胶膜。洗脱缓冲液(50–200 µl)上样到QIAamp硅胶膜,1分钟后离心洗脱纯的DNA。

See figures

Applications

QIAamp DSP DNA Blood Mini Kit使用经验证的QIAamp技术从新鲜或冷冻的全血中纯化DNA,其中血液是使用柠檬酸盐或EDTA处理过的。

Supporting data and figures

Specifications

FeaturesSpecifications
ApplicationsPCR, qPCR, real-time RT-PCR, microarray
Main sample typeWhole blood
Elution volume50–200 µl
Purification of total RNA, miRNA, poly A+ mRNA, DNA or proteinGenomic DNA
CE/FDA/IVD compatibleCE/IVD
FormatSpin columns
Sample amount200 µl
ProcessingManual (centrifugation or vacuum)
TechnologySilica technology

Resources

性能数据 (1)
QIAamp DSP DNA Blood Mini Performance Characteristics_V3_In Vitro Diagnostic use according to the Regulation (EU) 2017/746 on in vitro diagnostics medical devices
 
安全数据表 (1)
Download Safety Data Sheets for QIAGEN product components.
试剂盒操作手册 (1)
QIAamp DSP DNA Blood Mini Handbook_V3_In Vitro Diagnostic use according to the Regulation (EU) 2017/746 on in vitro diagnostics medical devices
 
Safety Data Sheets (1)
Certificates of Analysis (1)
Kit Handbooks (1)
QIAamp DSP DNA Blood Mini Handbook_V3_In Vitro Diagnostic use according to the Regulation (EU) 2017/746 on in vitro diagnostics medical devices
 
Performance Data (1)
QIAamp DSP DNA Blood Mini Performance Characteristics_V3_In Vitro Diagnostic use according to the Regulation (EU) 2017/746 on in vitro diagnostics medical devices
 

FAQ

What is the difference between QIAGEN Protease and QIAGEN Proteinase K provided in various QIAamp Kits?

QIAGEN Proteinase K is a subtilisin-type protease, which cleaves at the carboxyl side of hydrophobic, aliphatic and aromatic amino acids. It is particularly suitable for short digestion times. It possesses a high specific activity over a wide range of temperatures and pH values with substantially increased activity at higher temperature. Soluble calcium is not essential for enzymatic activity. This means that EDTA, which is used to inhibit Mg2+-dependent enzymes such as nucleases, will not inhibit Proteinase K activity.

QIAGEN Protease is a broad-specificity Serine protease with high activity, cleaving preferentially at neutral and acidic residues. It is an economical alternative to Proteinase K for isolation of native DNA and RNA from a variety of samples.

FAQ ID -761
What can be used as an alternative to the A260 measurement for quantification of small amounts of RNA and DNA?

Small amounts of RNA and DNA may be difficult to measure spectrophotometrically. Fluorometric measurements, or quantitative RT-PCR and PCR are more sensitive and accurate methods to quantify low amounts of RNA or DNA.

Fluorometric measurements are carried out using nucleic acid binding dyes, such as RiboGreen® RNA Quantitation Reagent for RNA, and PicoGreen® DNA Quantitation Reagent for DNA (Molecular Probes, Inc.).

FAQ ID -728
How do I safely inactivate biohazardous flow-through material?

Always dispose of potentially biohazardous solutions according to your institution’s waste-disposal guidelines. Although the lysis and binding buffers in QIAamp, DNeasy, and RNeasy kits contain chaotropic agents that can inactivate some biohazardous material, local regulations dictate the proper way to dispose of biohazards. DO NOT add bleach or acidic solutions directly to the sample-preparation waste. Guanidine hydrochloride in the sample-preparation waste can form highly reactive compounds when combined with bleach.
Please access our Material Safety Data Sheets (MSDS) online for detailed information on the reagents for each respective kit.

FAQ ID -12