JAK2 RGQ PCR Kit RUO

采用real-time PCR对JAK2 V617F/G1849T突变进行定量检测

S_1084_5_GEN_V2

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JAK2 RGQ PCR Kit (24) RUO

Cat. No. / ID:  673613

For 24 reactions: V617F Positive Control, V617F Negative Control, 2 sets of standards plasmid dilutions JAK2 wild-type and JAK2 V617F, qPCR master-mix, 2 Primers and Probe Mixes JAK2 wild-type and JAK2 V617F including internal control
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参考文献 Jovanovic, J.V.et al. (2013) Establishing optimal quantitative-polymerase chain reaction assays for routine diagnosis and tracking of minimal residual disease in JAK2-V617F-associated myeloproliferative neoplasms: a joint European LeukemiaNet/MPN&MPNr-EuroNet (COST action BM0902) study. Leukemia 27, 2032.
仅作研究用途。不用于诊断程序。任何表述或表示都并非旨在提供诊断、预防或治疗疾病的信息。

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✓ Knowledgeable and professional Product & Technical Support

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Features

  • 对JAK2 V617F进行可靠灵敏的定量
  • Larsen引物与最新的国际专业推荐相符(1)
  • 提供内参
  • 即用型溶液包含QIAGEN预混液
  • 简单易用的实验方案经过验证可用于QIAsymphony SP和Rotor-Gene Q 5plex HRM
  • 经过优化,反应时间缩短

Product Details

JAK2 RGQ PCR试剂盒可在Rotor-Gene Q 5plex HRM上进行real-time PCR反应。该试剂盒中的试剂经过优化,能够对基因组DNA中的JAK2 V617F/G1849T突变进行灵敏、可靠的定量检测。

Performance

为获得更高的灵敏性和可重复性,JAK2 RGQ PCR Kit经过优化,采用符合国际推荐标准的预稀释的质粒标准品、引物和探针混合溶液,检测JAK2 V617F/G1849T突变。灵敏检测的阈值(LOD)为0.042%。

Principle

JAK2 RGQ PCR Kit为即用型试剂盒,能够对基因组DNA中SNP导致的JAK2 V617F/G1849T体细胞突变进行定量检测。该试剂盒通过PCR和ARMS技术,在Rotor-Gene Q 5plex HRM平台上进行检测。

具有高灵敏度、高重复性的特点。JAK2 RGQ PCR Kit为每个突变的JAK2 V617F和野生型JAK2等位基因提供4个质粒标准品稀释液。这些标准品能够确保准确定量JAK2 V617F和JAK2野生型。检测结果会显示JAK2 V617F占总JAK2的比例、以及突变检测状态的LOD值。

Procedure

JAK2 RGQ PCR Kit可在Rotor-Gene Q 5plex HRM平台上定量检测JAK2 V617F/G1849T突变。第一步是手动、或在QIAsymphony平台自动抽提DNA,并检测其量与品质。第二步通过real-time PCR扩增基因组DNA,试剂盒中提供所有这一步所需的试剂。只需按照试剂盒操作手册中的实验方案,即可在Rotor-Gene Q 5plex HRM上便利的开始反应。

Applications

JAK2 RGQ PCR Kit可对JAK2 V617F/G1849T突变进行定量检测。该试剂盒仅适用于科研。

Resources

试剂盒操作手册 (1)
Package Insert (1)
For more information about this product, please refer to JAK2 RGQ PCR Kit Handbook (www.qiagen.com/JAK2).
安全数据表 (1)
Download Safety Data Sheets for QIAGEN product components.
Safety Data Sheets (1)
Certificates of Analysis (1)