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工具和计算器
Home
applications
分子生物学相关酶产品
工具和计算器
PCR 预混液计算器
PCR 预混液计算器
1.选择要使用的 PCR 产品。
Custom
2.填写您要使用的模板 DNA 的体积、您打算运行的 PCR 反应的次数和每个反应的体积。 您可以额外添加一些移液用体积。
每次反应的模板体积
µL
反应体积
µL
反应次数
额外反应
总体积
µL
3.填写各组分的储备浓度和所需的最终浓度。
浓度
体积
储备液
最终
每最大值
用于预混液
PCR Buffer
x
x
Taq Polymerase
U/uL
U/uL
dNTP-Mix
mM
mM
Primer A
uM
uM
Primer B
uM
uM
可选:MgCl2
mM
mM
Optional Tracer
x
x
可选: Q-Solution
x
x
键入您的组分
键入您的组分
键入您的组分
水
Template DNA
体积
每最大值
用于预混液
PCR Buffer
0 µL
0 µL
Taq Polymerase
0 µL
0 µL
dNTP-Mix
0 µL
0 µL
Primer A
0 µL
0 µL
Primer B
0 µL
0 µL
可选:MgCl2
0 µL
0 µL
Optional Tracer
0 µL
0 µL
可选: Q-Solution
0 µL
0 µL
水
Template DNA
不含模板的体积
含模板的体积