Rotor-Gene Q Accessories

For use with the Rotor-Gene Q for outstanding performance in real-time PCR

Features

Useful Resources

Using the KRAS PCR Kit on the Rotor-Gene® Q Instrument - (EN) Using the EGFR PCR Kit on the Rotor-Gene® Q Instrument - (EN) (EN) - Maximizing PCR and RT-PCR success — Third Edition

Products

Rotor-Gene Q Accessories are for use with the Rotor-Gene Q thermal cycler.

Rotor-Disc 72 Rotor

Cat. No. / ID: 9018899

For holding Rotor-Disc 72 discs with reaction volumes of 20–25 µl in the Rotor-Gene Q; requires Rotor-Disc 72 Locking Ring

Rotor-Disc 100 Locking Ring

Cat. No. / ID: 9018896

For locking a Rotor-Disc 100 in the Rotor-Disc 100 Rotor

Rotor-Disc Pipetting Aid

Cat. No. / ID: 9018897

Aid for marking well during manual reaction setup on a Rotor-Disc Loading Block

Rotor-Disc 100 Rotor

Cat. No. / ID: 9018895

For holding Rotor-Disc 100 discs with reaction volumes of 15–25 µl in the Rotor-Gene Q; requires Rotor-Disc 100 Locking Ring

Rotor-Disc Heat Sealer 110V

Cat. No. / ID: 9018898

Heat sealing instrument for use with Rotor-Discs; requires Rotor-Disc 72 or 100 Loading Block

Loading Block 72 x 0.1 ml Multi-channel

Cat. No. / ID: 9018902

Aluminum block for reaction set-up with multichannel pipets in 72 x 0.1 ml tubes

Loading Block, Rotor-Disc 100 Easylift Ejector

Cat. No. / ID: 9020286

Ejector to be used in combination with Loading Block, Rotor-Disc 100 Easylift (cat. no. 9020287)

Loading Block 96 x 0.2 ml Tubes

Cat. No. / ID: 9018905

Aluminum block for manual reaction set-up in a standard 8 x 12 array using 96 x 0.2 ml tubes

Rotor-Disc 100 (30)

Cat. No. / ID: 981311

30 individually wrapped discs for 3000 reactions of 15–25 µl

Locking Ring 72-Well Rotor

Cat. No. / ID: 9018904

For locking Strip Tubes and Caps, 0.1 ml in the 72-Well Rotor

Loading Block, Rotor-Disc 100 Easylift

Cat. No. / ID: 9020287

Aluminium block for manual and automated reaction setup in Rotor-Disc 100 discs. The block covers 2 standard SBS positions in a vertical orientation on the QIAgility instrument worktable; ejection of the Rotor-Disc 100 is made convenient by using the Easylift ejector (cat. no. 9020286)

Rotor-Disc Heat Sealer 230V

Cat. No. / ID: 9019725

Heat sealing instrument for use with Rotor-Discs; requires Rotor-Disc 72 or 100 Loading Block

Loading Block 72 x 0.1 ml Tubes

Cat. No. / ID: 9018901

Aluminum block for manual reaction setup with a single-channel pipet in 72 x 0.1 ml tubes

PCR Tubes, 0.2 ml (1000)

Cat. No. / ID: 981005

1000 thin-walled tubes for 1000 reactions of 20–50 µl

Rotor-Disc 72 (24)

Cat. No. / ID: 981301

24 individually wrapped discs for 1728 reactions of 20–25 µl

PCR Tubes, 0.2 ml (10000)

Cat. No. / ID: 981008

10 x 1000 thin-walled tubes for 10,000 reactions of 20–50 µl

Rotor-Disc 100 (300)

Cat. No. / ID: 981313

10 x 30 individually wrapped discs for 30,000 reactions of 15–25 µl

Rotor-Disc Heat Sealing Film (60)

Cat. No. / ID: 981601

60 films for sealing Rotor-Disc 100 or Rotor-Disc 72 discs

Rotor-Disc 72 (240)

Cat. No. / ID: 981303

10 x 24 individually wrapped discs for 17,280 reactions of 20–25 µl

Rotor-Disc Heat Sealing Film (600)

Cat. No. / ID: 981604

10 x 60 films for sealing Rotor-Disc 100 or Rotor-Disc 72 discs

Rotor-Disc 72 Locking Ring

Cat. No. / ID: 9018900

For locking a Rotor-Disc 72 in the Rotor-Disc 72 Rotor

36-Well Rotor Locking Ring

Cat. No. / ID: 9018906

For locking PCR Tubes, 0.2 ml, in the 36-Well Rotor

Rotor Holder

Cat. No. / ID: 9018908

Metal free-standing holder for assembling tubes and Rotor-Discs into rotors

36-Well Rotor

Cat. No. / ID: 9018907

For holding PCR Tubes, 0.2 ml, with reaction volumes of 20–50 µl; requires 36-Well Rotor Locking Ring

Rotor-Disc 72 Loading Block

Cat. No. / ID: 9018910

Aluminium block for manual and automated reaction setup in Rotor-Disc 72 discs

72-Well Rotor

Cat. No. / ID: 9018903

For holding Strip Tubes and Caps, 0.1 ml, with reaction volumes of 10–50 µl; requires Locking Ring 72-Well Rotor

Strip Tubes and Caps, 0.1 ml (2500)

Cat. No. / ID: 981106

10 x 250 strips of 4 tubes and caps for 10,000 reactions of 10–50 µl

Strip Tubes and Caps, 0.1 ml (250)

Cat. No. / ID: 981103

250 strips of 4 tubes and caps for 1000 reactions of 10–50 µl

Product Details

Rotor-Gene Q Accessories are for use with the Rotor-Gene Q for outstanding performance in real-time PCR.

Resources

PCR 実験における重要項目と新技術

Addressing critical factors and new solutions

FAQ

No. The master mixes in Rotor-Gene Kits contain dTTP instead of dUTP. If UNG treatment is required, we recommend using QuantiTect +UNG Kits. QuantiTect Kits are also compatible with the Rotor-Gene Q; however, the kits require a significantly longer cycling time.

FAQ ID -2117

Yes. The optimized master mix of the Rotor-Gene Multiplex PCR Kit ensures that all gene targets in a multiplex reaction are amplified with the same efficiency and sensitivity as in corresponding singleplex reactions, independent of starting copy numbers.

FAQ ID -2129

The Rotor-Gene Multiplex PCR Kit has been developed primarily for use with TaqMan® probes. Protocols for probes other than TaqMan® probes have not been tested.

See trademarks.

FAQ ID -2130

We strongly recommend use of the dedicated Rotor-Gene Q Accessories, e.g., PCR Tubes 0.2 ml or Strip Tubes and Caps 0.1 ml provided by QIAGEN. These tubes are designed to give low background and perfectly match the Rotor-Gene Q requirements.

FAQ ID -2085

The Rotor-Gene Q instrument can be used with two different rotor formats: using tubes or rotor-dics. Tubes can be run in 36 and 72-well rotors and rotor-discs in Rotor-Disc 72 and Rotor-Disc 100 rotors. When programming the temperature profile please make sure the correct rotor type is selected.

FAQ ID -1519

During the sealing process, the "orange Sealing LED" is On. When sealing is finished, the "orange sealing LED starts blinking and a long beep sounds”. When the Rotor-disc adaptor has been pulled out of the Heat Sealer unit, wait until the seal has cooled down before removing the excess seal, which is about 20 seconds. Remove the excess sealing film by pushing down to detach. Never pull up on the excess film to remove it.

FAQ ID -3179

There are several manufacturers of high-quality real-time cyclers. These include QIAGEN's Rotor-Gene Q, Applied Biosystems, BioRad, Stratagene, Eppendorf, Roche, TaKaRa, Fluidigm, and Cepheid. The important thing to keep in mind is that, once you select an instrument to use, you must use compatible Rotor-Gene Discs and tubes, 96 or 384 well plates, and qPCR master mixes that are optimized for use in that particular instrument. For example, QIAGEN's Rotor-Gene SYBR Green, Probe, and Multiplex real-time master mixes.

FAQ ID -2670

The majority of the Rotor-Gene Kit data shown in our literature has been generated with the help of the QIAgility instrument. We did not observe any problems during the pipetting steps.

FAQ ID -2116

Rotor-Gene Kits are specifically developed for the Rotor-Gene Q PCR Cycler. The unique rotary system of the cycler combined with the kits’ proprietary buffer system enable ultrafast cycling. Rotor-Gene Kits do not contain ROX dye since no normalization to a passive reference is required. Also, Rotor-Gene Kits do not contain dUTP; therefore, UNG pretreatment is not possible.

FAQ ID -2119

The Globally Harmonized System (GHS) is an initiative by the United Nations aiming to harmonize chemical hazard communication worldwide. Hazards symbols accompanying products are changing to those of the GHS. Information on the adoption of this new system is available on our GHS Hazard Labeling pages.

FAQ ID - 3430