EZ1 RNA Tissue Mini Kit

The EZ1 RNA Tissue Mini Kit contains all required reagents and labware for automated purification of total RNA from up to 10 mg standard tissue samples using magnetic particle technology. Reagents are supplied in prefilled reagent cartridges, which ensures speed and convenience in loading the instrument. The EZ1 RNA Tissue Kit can be used with the EZ1 Advanced XL, the EZ1 Advanced, or the BioRobot EZ1.

This EZ1 Kit can also be automated with EZ2 Connect, which features cardless protocols, expanded applications and remote connectivity options. Click here to learn more about the EZ2 Connect, or contact your sales representative to discover how easily you can get an EZ2 Connect for your lab.

The yields and high quality of total RNA purified using the EZ1 RNA Tissue Mini Kit are consistent and reproducible (see figures " Efficient purification and reproducible yields" and " Consistent highly pure RNA").

Automated purification with the EZ1 RNA Tissue Mini Kit requires use of the EZ1 Advanced XL and the EZ1 Advanced XL RNA Card, or the EZ1 Advanced and the EZ1 Advanced RNA Card, or the BioRobot EZ1 and the EZ1 RNA Card. EZ1 cards are preprogrammed with protocols that guide the user through worktable setup and provide the instrument with commands for the purification procedure.

Using silica-coated magnetic-particle technology, the easy-to-use instrument allows purification of 1-14 samples (EZ1 Advanced XL) or 1-6 samples (EZ1 Advanced and BioRobot EZ1) in a single run. Presealed reagent cartridges and the automatic sensor-controlled lock of the instrument door reduce the chances of environmental contamination or degradation due to RNases. The automated procedure provides high repeatability and linear yields over a range of starting amounts. The flexibility to purify high-quality total RNA from a wide range of tissue types ensures that samples can be purified for a range of gene expression analysis applications. RNA yield using the EZ1 RNA Tissue Mini Kit is dependent upon many variables, including sample weight, tissue type and developmental state.

Tissues are first lysed and homogenized in Buffer RLT. RNA is then purified in one step on the instrument following the automated EZ1 RNA procedure. For certain RNA applications that are sensitive to very small amounts of DNA, an integrated DNase digestion step removes genomic DNA contamination. Total RNA is eluted in 200 µl, 100 µl, or 50 µl, ready to use in all downstream applications.

The high-quality RNA obtained using the EZ1 RNA Tissue Mini Kit is suited for use in many applications, such as:

• Gene expression analysis using real-time RT-PCR technologies
• Gene expression analysis using microarray technologies
• Oncology and pathology research