What are the expected PCR results when using EpiTect Control DNA on untreated or bisulfite-converted DNA?
FAQ ID -2011
Expected PCR results when using the EpiTect Control DNA Set in combination with the EpiTect MSP Kit can be found in the table below:
| Type of DNA |
Primer for unmethylated & unconverted genomic DNA |
Primer for unmethylated & bisulfite converted target DNA |
Primer for methylated & bisulfite converted target DNA |
| Unmethylated & untreated control DNA |
PCR product |
NO PCR product |
NO PCR product |
| Unmethylated & bisulfite converted control DNA |
NO PCR product |
PCR product |
NO PCR product |
| Methylated & bisulfite converted control DNA |
NO PCR product |
NO PCR product |
PCR product |
| No template control |
NO PCR product |
NO PCR product |
NO PCR product |