MagAttract Viral RNA M48 Kit

The MagAttract Viral RNA M48 Kit provides automated purification of viral RNA on the BioRobot M48 using proven MagAttract magnetic particle technology. Variable elution volumes enable easy optimization of viral RNA concentration for downstream applications. Viral RNA purification and magnetic separation take place in the pipet tips, increasing the efficiency of the procedure.

The yield of viral RNA per sample with the MagAttract Viral RNA M48 Kit is normally below 1 µg, and is therefore difficult to determine with a spectrophotometer. Quantitative amplification methods are recommended for determination of yields.

The MagAttract Viral RNA M48 Kit provides fully automated purification of viral RNA from serum and plasma. MagAttract technology combines the speed and efficiency of silica-based RNA purification with the convenient handling of magnetic particles. Samples are processed in the BioRobot M48 in 1.5 ml or 2 ml sample tubes. Up to 48 samples in increments of 6 samples can be processed per run. The sample source can be up to 300 µl serum or plasma. The yield of purified RNA depends on the sample type and virus titer.

In the MagAttract Viral RNA M48 procedure, viral RNA binds to the silica surface of the magnetic particles in the presence of a chaotropic salt. Viral RNA bound to the particles is then efficiently washed using two different wash buffers. High-quality viral RNA is eluted at 65ºC in an RNase-free elution buffer.

The high-quality RNA obtained using the MagAttract Viral RNA M48 Kit and the BioRobot M48 is suited for use in many downstream applications, such as:

• Viral epidemiology and genotyping
• Infectious disease research