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Transfection Cell Database

Our searchable database contains data from researchers like yourself who have shared their experimental results with us. All available information is shown in the detailed view. As always, QIAGEN cannot verify data supplied from outside sources.

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Transfection protocols

To download a QIAGEN transfection protocol for your cell type, nucleic acid, and plate format of interest, visit the TransFect Protocol Database.

 

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Nucleic Acid Transfection Reagent
Cell Line Type of Transfection
Cell Line Species/Tissue

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Cell Records

Cell Line Cell Line Species/Tissue Nucleic Acid Reagent Transfection Type Plate Format
293T  Monkey/ DNA  PolyFect  Transient  100-mm dish 
293T  Human/Kidney Epithelial DNA  PolyFect  Transient  35mm tissue culture dishes 
2fTGH  Human/fibroblast DNA  PolyFect  Transient  6-well plate 
3 B/2  Opossum/Kidney proximal tubulus DNA  Effectene  Transient  12-well plate 
A375  Human/Melanoma/ DNA  Attractene  Transient  24-well 
A375m2   Human/Melanoma siRNA (dsRNA)  HiPerFect    6-well plate 
A375m2   Human/Melanoma siRNA (dsRNA)  HiPerFect    12-well plate  
A549  Human/Carcinoma lung epithelial siRNA (dsRNA)  HiPerFect    96-well 
A549  Human/Carcinoma lung epithelial DNA  Effectene  Transient  60-mm dish 
A549  Human/Carcinoma lung epithelial DNA  Effectene  Transient  60-mm dish 
A549  Human/Carcinoma lung epithelial DNA  SuperFect  Stable  60-mm dish 
A549  Human/ siRNA (dsRNA)  HiPerFect HTS    96-well 
A549  Human/ siRNA (dsRNA)  HiPerFect HTS    384-well 
A549  human/lung carcinoma DNA  Attractene  Transient  24-well 
A549  human/lung DNA  Attractene  Transient  24-well plate 
Acanthamoeba  Acanthamoeba castellanii/Neff strain DNA  SuperFect  Transient  6-well in 3ml culture medium 
AGS  Human/gastric adenocarcinoma siRNA (dsRNA)  HiPerFect    96-Well 
AGS  Human/ siRNA (dsRNA)  HiPerFect HTS    96-well 
AGS  Human/ siRNA (dsRNA)  HiPerFect HTS    384-well 
Alexander  hepatocellular carcinoma/ siRNA (dsRNA)  HiPerFect    6-well 
ARPE-19  Human/Retina siRNA (dsRNA)  RNAiFect    6-well 
ATDC5  Mouse/Embryo siRNA (dsRNA)  RNAiFect    24-well 
AtT-20  mouse/pituitary tumor DNA  Effectene  Stable  100-mm dish 
AtT-20  mouse/pituitary tumor DNA  SuperFect  Stable  100-mm dish 
B16  Mouse/Melanocyte DNA  SuperFect  Stable  24-well plate 
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