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RT2 qPCR Primer Assays

For accurate and reliable gene expression analysis using laboratory-verified assays

  • Complete genome coverage for multiple species
  • Amplifies a single amplicon with uniform PCR efficiency
  • Less than 5 minutes hands-on time

RT² qPCR Primer Assays are specifically designed and experimentally verified for real-time PCR analysis. The rigorous assay verification criteria ensure PCR specificity and efficiency for reliable and accurate gene expression analysis results.

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RT2 qPCR Primer Assays are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.


Principle

RT² qPCR Primer Assays use SYBR® Green-based quantitative real-time PCR technology to provide a sensitive and reliable tool for gene expression analysis.

Each assay utilizes a proprietary and experimentally verified algorithm for the design of gene-specific qPCR primers with uniform PCR efficiency and amplification conditions. Each lot of every assay is further wet-bench tested for real-time PCR performance for specificity and amplification efficiency. Amplification of a single product of the correct size with high PCR efficiency (>90%) is guaranteed when the assays are used with RT² SYBR® Green qPCR Mastermixes. The uniform PCR efficiencies and PCR conditions of the RT² qPCR Primer Assays provide an accurate and scalable solution for multiple gene expression analyses.

 

Applications
RT2 qPCR Primer Assays are highly suited for applications including validation of microarray-derived gene expression data, confirming gene expression knockdown by RNAi, identifying and confirming disease-associated biomarkers, and monitoring phenotypic changes related to gene expression. Multiple RT2 qPCR Primer Assays can also be used to examine a focused panel of genes.

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常见问题 (30)
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试剂盒操作手册 (2)
For cDNA synthesis from 96 samples
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For gene expression analysis by real-time RT-PCR
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仪器技术参数 (1)
For pathway-focused gene expression analysis
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产品介绍与指南 (1)
Simultaneously profile mRNA, miRNA and lncRNA using a simple, complete workflow
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SI01487150 siRNA PPR48689A RT² qPCR Primer Assay SI04716152 siRNA SI04716159 siRNA SI01487157 siRNA

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Name RT² qPCR Primer Assay for rat Alb (PPR48689A)
Official symbol Alb
Official name albumin
Species Rat (Rattus norvegicus)
Entrez Gene ID 24186
Detected transcript NM_134326 (2020 bp)
Band Size 128 bp (NM_134326)
Reference Position 1 1690 (NM_134326)

1 The Reference Position is a position contained within the sequence of the amplicon relative to the start of the relevant RefSeq sequence.