text.skipToContent text.skipToNavigation

RT2 qPCR Primer Assays

For accurate and reliable gene expression analysis using laboratory-verified assays

  • Complete genome coverage for multiple species
  • Amplifies a single amplicon with uniform PCR efficiency
  • Less than 5 minutes hands-on time

RT² qPCR Primer Assays are specifically designed and experimentally verified for real-time PCR analysis. The rigorous assay verification criteria ensure PCR specificity and efficiency for reliable and accurate gene expression analysis results.

Advanced search settings



Species:
Products
Product Product no. Cat. no. List price:
 
 
show details
    varies
Can't order online?
To place an order via phone, email or for requesting a quote, please provide the product’s name, number and catalog number.

RT2 qPCR Primer Assays are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.


Principle

RT² qPCR Primer Assays use SYBR® Green-based quantitative real-time PCR technology to provide a sensitive and reliable tool for gene expression analysis.

Each assay utilizes a proprietary and experimentally verified algorithm for the design of gene-specific qPCR primers with uniform PCR efficiency and amplification conditions. Each lot of every assay is further wet-bench tested for real-time PCR performance for specificity and amplification efficiency. Amplification of a single product of the correct size with high PCR efficiency (>90%) is guaranteed when the assays are used with RT² SYBR® Green qPCR Mastermixes. The uniform PCR efficiencies and PCR conditions of the RT² qPCR Primer Assays provide an accurate and scalable solution for multiple gene expression analyses.

 

Applications
RT2 qPCR Primer Assays are highly suited for applications including validation of microarray-derived gene expression data, confirming gene expression knockdown by RNAi, identifying and confirming disease-associated biomarkers, and monitoring phenotypic changes related to gene expression. Multiple RT2 qPCR Primer Assays can also be used to examine a focused panel of genes.

You are not authorized to download the resource

FAQs (30)
FAQ ID -2684
View
FAQ ID -2682
View
FAQ ID -2680
View
FAQ ID -2681
View
FAQ ID -2689
View
FAQ ID -2690
View
FAQ ID -2688
View
FAQ ID -2685
View
FAQ ID -2686
View
FAQ ID -2679
View
FAQ ID -2678
View
FAQ ID -2675
View
FAQ ID -2694
View
FAQ ID -2695
View
FAQ ID -2693
View
FAQ ID -2691
View
FAQ ID -2692
View
FAQ ID -2696
View
FAQ ID -2655
View
FAQ ID -2715
View
FAQ ID -2716
View
FAQ ID -2712
View
FAQ ID -2713
View
FAQ ID -2714
View
FAQ ID -2709
View
FAQ ID -2710
View
FAQ ID -2711
View
FAQ ID -2706
View
FAQ ID -2707
View
FAQ ID -2708
View
Kit Handbooks (2)
For cDNA synthesis from 96 samples
Show details
For gene expression analysis by real-time RT-PCR
Show details
Instrument Technical Documents (1)
For pathway-focused gene expression analysis
Show details
Brochures & Guides (1)
Simultaneously profile mRNA, miRNA and lncRNA using a simple, complete workflow
Show details
Name RT² qPCR Primer Assay for Human WRN (PPH02160A)
Official symbol WRN
Official name Werner syndrome, RecQ helicase-like
Species Human (Homo sapiens)
Entrez Gene ID 7486