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RT2 qPCR Primer Assays

For accurate and reliable gene expression analysis using laboratory-verified assays

  • Complete genome coverage for multiple species
  • Amplifies a single amplicon with uniform PCR efficiency
  • Less than 5 minutes hands-on time

RT² qPCR Primer Assays are specifically designed and experimentally verified for real-time PCR analysis. The rigorous assay verification criteria ensure PCR specificity and efficiency for reliable and accurate gene expression analysis results.

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RT2 qPCR Primer Assays are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.


RT² qPCR Primer Assays use SYBR® Green-based quantitative real-time PCR technology to provide a sensitive and reliable tool for gene expression analysis.

Each assay utilizes a proprietary and experimentally verified algorithm for the design of gene-specific qPCR primers with uniform PCR efficiency and amplification conditions. Each lot of every assay is further wet-bench tested for real-time PCR performance for specificity and amplification efficiency. Amplification of a single product of the correct size with high PCR efficiency (>90%) is guaranteed when the assays are used with RT² SYBR® Green qPCR Mastermixes. The uniform PCR efficiencies and PCR conditions of the RT² qPCR Primer Assays provide an accurate and scalable solution for multiple gene expression analyses.


RT2 qPCR Primer Assays are highly suited for applications including validation of microarray-derived gene expression data, confirming gene expression knockdown by RNAi, identifying and confirming disease-associated biomarkers, and monitoring phenotypic changes related to gene expression. Multiple RT2 qPCR Primer Assays can also be used to examine a focused panel of genes.
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SI03047807 siRNA SI03046589 siRNA SI00256935 siRNA SI03049102 siRNA PPR49717A RT² qPCR Primer Assay


Name RT2 PCR Primer Set for Rat Ntf4 (PPR49717A)
Official symbol Ntf4
Official name neurotrophin 4
Species Rat (Rattus norvegicus)
Entrez Gene ID 25730
Detected transcript NM_013184 (1338 bp)
Band Size 134 bp (NM_013184)
Reference Position 1 625 (NM_013184)

1 The Reference Position is a position contained within the sequence of the amplicon relative to the start of the relevant RefSeq sequence.

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