For rapid, high-precision automated PCR setup
  • Automated PCR setup in all formats
  • Convenient, easy-to-use software
  • Pre-optimized experiment setup
  • Standardized results and increased productivity
  • Elimination of manual pipetting steps
The QIAgility is a compact benchtop instrument that enables rapid, high-precision setup of PCR experiments with ready-to-use software for ease and convenience. The software provides step-by-step guidance for worktable setup and automatic calculation of all mixes, eliminating the need to program pipetting steps. Just select the kit and cycler, define your targets and start the run. For added flexibility the QIAgility is operated via a laptop computer.

Explore the virtual demo to learn more about the QIAgility.

​QIAgility operates in conjunction with QIAgility Accessories and QIAgility Consumables.
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QIAgility System HEPA/UV (incl. PC)
Robotic workstation for automated PCR setup (with UV light and HEPA filter), notebook computer, and QIAgility Setup Manager Software: includes installation and training, 1-year warranty on parts and labor
9001532
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QIAgility Priority Package HEPA/UV (incl. PC)
Robotic workstation for automated PCR setup (with UV light and HEPA filter), notebook computer, and QIAgility Setup Manager Software: includes Priority Package with software, installation, training, 2-year warranty on parts and labor, and 2 preventive maintenance visits
9001903
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QIAgility Priority Package Plus HEPA/UV (incl. PC)
Robotic workstation for automated PCR setup (with UV light and HEPA filter), notebook computer, and QIAgility Setup Manager Software: includes Priority Package with software, installation, training, 3-year warranty on parts and labor, and 3 preventive maintenance visits
9001904
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The QIAgility is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

The QIAgility, if used in combination with QIAGEN kits indicated for use with the QIAgility instrument, is intended for the applications described in the respective QIAGEN kit handbooks. If the QIAgility instrument is used with kits other than QIAGEN kits, it is the user’s responsibility to validate the performance of such product combination for any particular application.


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Exceptional pipetting precision and accuracy spanning over a broad volume range|Unrivaled pipetting precision and reproducibility of the QIAgility for master mix solutions|Comparison of manual and automated assay setup using series dilutions|High system linearity of QIAgility|
The Artel MVS® system was employed to determine the pipetting precision of the QIAgility instrument operated by the QIAgility Setup Manager software. The QIAgility dispensed the Artel solutions (selected according to the Artel MVS User Manual) into the Artel buffer. Three runs were performed on three QIAgility instruments that were used to pipet an entire volume range (1, 2, 5, 10, 20, 50, 100, and 200 µl), in replicates of 12. For each run and volume (n=12) the coefficient of variation (CV) was calculated to determine the pipetting precision of the QIAgility. The data shows the mean of the CV values (bars; in %) ± standard deviation (S.D.) of the CV values as error bars. The red lines depict the claimed specifications.
The indicated CV values (%) demonstrate that the QIAgility instrument performs within its claimed specifications and even better.|A reaction mix was prepared manually using 5 parts of 2x Rotor-Gene SYBR Green PCR Master Mix and 3 parts of RNase free water (ready-made reaction mix). An experiment was then defined in the QIAgility Setup Manager for the Rotor-Gene® SYBR® Green PCR Kit with a total reaction volume of 25 µl and a sample input volume of 5 µl, resulting in the transfer of 20 µl of the ready-made reaction mix. The transfers were made into pre-weighed 200 µl single tubes (n=36 per instrument). The tubes were weighed again after the transfer and the weight difference was considered to calculate the transferred volume. For each instrument, the pipetting precision was determined as CV (%). The data shows the mean of the CV values (bars; in %) ± S.D. (error bars) of each individual instrument. The pipetting precision or CV, respectively, was nearly 3 % for all instruments tested.
The indicated CV values (%) demonstrate that the QIAgility instrument has high performance rate in terms of precision and repeatability when pipetting ready-made reaction mix as shown on all 4 instruments.|A QuantiNova™ SYBR® Green assay setup was performed using the QIAgility operated with its Setup Manager software and this was compared to a manual setup done in parallel. A manually prepared 1:10 dilution series from a stock of human gDNA with a concentration of 60 ng/µl was used in a ß-actin assay. Each dilution was tested in quadruplicates (n=4). The manual and QIAgility-prepared assay points were amplified in the same Rotor-Gene Q run. The data is represented as a box plot of the Ct value calculated using the Q-Rex software for each template concentration and setup employed.
A highly similar PCR performance is observed between manual and automated assay setup over a series of sample concentrations.
|Human gDNA with a stock concentration of 1.5 µg/µl was diluted with the QIAgility six times in the ratio of 1:2 per dilution step using RNase free water as diluent. The dilution series was prepared in replicates of 3. The nucleic acid concentration of the dilutions was determined using the QIAxpert® UV/VIS spectrophotometer (DNA QIAamp Application). The mean measured quantifications were plotted against the mean theoretical nucleic acid concentrations assuming a perfect 1:2 dilution. The resulting plot demonstrates the exceptional linearity of QIAgility dilution with a high regression coefficient of >0.999.
Quantifications of serial dilutions of gDNA prepared using the QIAgility are in high concordance with actual concentrations.
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Performance
The QIAgility fully automates proven QIAGEN PCR kits for use with various workflows, and offers pure pipetting precision, enhanced safety features, and exceptional reliability and reproducibility.

Unmatched pipetting precision with QIAgility

The MVS® (Artel, Inc.) is a third-party system providing performance assessment of liquid handling instruments used for assay setup.
The experimental data determined by the MVS for the QIAgility instrument operated by the QIAgility Setup Manager Software demonstrates that the instrument easily exceeds its own claimed specifications for the accuracy and precision for all volumes analyzed (see figure “Exceptional pipetting precision and accuracy spanning over a broad volume range”). These results indicate that the QIAgility can produce automated, high-performance liquid dispensing results, while avoiding common errors that can be associated with manual pipetting and thereby reducing the time to result.
Regardless of whether you pipet water or QIAGEN ready-made reaction mix to set up a PCR experiment, the QIAgility instrument can dispense volumes that are highly precise and also generate significantly reproducible replicates (see figure “Unrivaled pipetting precision and reproducibility of the QIAgility for master mix solutions”).

High performance PCR setup using QIAgility

A QuantiNova SYBR Green PCR assay setup was performed manually and automated in parallel using the QIAgility along with its Setup Manager Software. The amplification run was done on the Rotor-Gene Q and resulting data analyzed using the Q-Rex Software. Consistent results were obtained from both manual and automated reaction setup using the same template dilutions (see figure “Comparison of manual and automated assay setup using series dilutions”).

Exceptional linearity of QIAgility

QIAgility exhibits excellent linearity and assures accurate and reliable use of sample for an assay. To assess the linearity of the system, pure gDNA was serially diluted using the QIAgility and measured for their concentrations on the QIAxpert instrument. The quantifications from the QIAxpert were plotted against the known concentration values, and the resulting plot demonstrates the exceptional linearity (with a regression coefficient of >0.999) of QIAgility (see figure “High system linearity of QIAgility”).



Principle
Automated PCR setup is rapid and reliable and eliminates manual pipetting steps that are typically prone to human error.

Liquid-handling tasks are performed by the single-channel pipetting head, which provides fast transfer of liquids with various types of plasticware. For example, 96 samples with the QuantiFast SYBR Green PCR Kit with 2 standard series can be setup in approximately 45 minutes. The worktable features a flexible layout and supports almost all kinds of cyclers and labware, giving high application versatility. Dynamic liquid-level sensing, facilitated by conductive filter tips offers maximum safety control.
 
The advanced design of the QIAgility delivers improved process safety, giving you peace of mind. A built-in sensor ensures that the instrument pauses when the hood is opened during a run, protecting users from moving parts. The UV light ensures effective worktable decontamination and the HEPA filter provides positive clean air pressure throughout PCR setup.

QIAgility is a compact benchtop instrument with a very small footprint. It fits easily onto most lab benches and can be readily moved into biosafety level laboratories. For added flexibility, QIAgility comes with a laptop computer. To reduce your work load, comprehensive run reports containing all run information are generated automatically during each protocol.

Procedure
Quick and easy setup of your PCR experiment with the new Setup Manager Software




​Let the QIAgility do the pipetting and save your valuable time



Applications
The instrument is highly suited for laboratories performing PCR and RT-PCR reactions with applications in: ​    
  • Gene Expression analysis
  • Genotyping
  • Cloning
  • Pathogen Detection
  • Food and veterinary testing
Please click here to get more information on which kits are currently supported by the new Setup Manager Software for gene expression analysis.

Check the instrument operating software and protocol files under product resources for more information.
Services
Is your plate or tube not supported in the QIAgility software? Contact QIAGEN Technical Services to request a new plate or tube to suit your workflow.
Feature
Specifications
Accessories (supplied) 2 x Adapter, 96 x 0.2 ml PCR Tubes
1 x Adapter, Flip-Cap 32 x 1.5 ml Tapered Base
1 x Adapter, 72 x 0.1 ml Strip Tubes
1 x Master Mix Block, 5 x 5 ml
1 x Master Mix Block, 1 x 5 ml, 4 x 2 ml
1 x Master Mix Block, 1 x 5 ml, 4 x 1.5 ml
1 x Reagent Block, 16 x 0.2 ml / 2 ml Flat / 1.5 ml Tapered Tubes
4 x Tip Rack Holder for 96-Tip Rack
The adapters are intended to hold tubes or plates.
Altitude Up to 2000 m (6500 ft.)
Communications USB and RS232
Conductive Filtered Tips 50 µl and 200 µl Conductive Filtered Tips for liquid-level sensing
Consumables (supplied) 10 racks of 50 µl Conductive Filtered Tips (960); 10 racks of 200 µl Conductive Filtered Tips (960); 25 x 5 ml tubes; 100 x 0.2 µl PCR tubes; 3 Tip Disposal Boxes
Dimensions Height: 45 cm (18 in.), instrument lid closed; Height: 86 cm (34 in.), instrument lid open; Width: 54 cm (21 in.); Depth: 63 cm (25 in.), without cables
Electrical 100–240 V AC, 250 VA, 50–60 Hz
Environmental class 3K2 (IEC 60721-3-3)
3M2 (IEC 60721-3-3)
HEPA filter Preinstalled dual-cartridge 3M Air-Mate HEPA (High Efficiency Particulate Air) filter system provides positive pressure clean air within the closed hood.
Humidity Operating humidity: 40–70%
Liquid-level sensing Minimum detection volume (10 µl in 200 µl PCR tubes). Only functional in combination with QIAGEN Conductive Filtered Tips.
Notebook (supplied) QIAgility comes with a notebook computer for added flexibility.
Pipetting head Single channel
Place of operation For indoor use only
Pollution level 2
Precision CV <1%, 5–200 µl (dry-well transfer)
CV <5%, 2–5 µl (wet-well transfer)
CV <10%, 1 µl
Software Supplied with unlimited user license. Free upgrades by Web download.
Table capacity 6 x SBS positions (127.76 x 85.48 mm) for adapters and Tip Rack Holders
1 x Master Mix Block
1 x Reagent Block
Temperature Operating temperature, 15–30 °C (59–86°F)
Tip disposal All used tips ejected externally — no waste accumulation on the worktable
Tip volume 1–200 µl
UV lamp Preinstalled UV lamp for worktable decontamination
Warranty 1-3 years warranty, depending on service package
Weight 41 kg (90.2 lb)

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Instrument User Manuals
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Important note regarding new QIAgility declaration of conformity and instrument symbols
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Supplementary user manual for use with the QIAgility Setup Manager Gene Expression Plug-in in combination with QIAgility Setup Manager software.
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For use with QIAgility and QIAgility Setup Manager Software
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For use with QIAgility Operating Software version 4.17.1
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Protocol Files
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These protocol files can only be used with QIAgility Operating software version 4.17 software and are not compatible with the new QIAgility Setup Manager software.
QIAgility Setup Manager software offers a complete solution for qPCR setup optimized for use with QIAGEN gene expression kits, such as QuantiTect, QuantiFast, QuantiNova and Rotor-Gene Kits.
The application spectrum of the QIAgility Setup Manager software will be expanded in the future with development of plug-ins for genotyping, pathogen testing, veterinary testing and food testing.

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Operating Software
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Instrument operating software including the QIAgility Setup Manager Gene Expression plug-in. This new software offers a complete solution for qPCR setup and is optimized for use with QIAGEN gene expression kits, such as QuantiTect, QuantiFast, QuantiNova and Rotor-Gene Kits. We recommend using this software for all gene expression applications.

For software installation instructions, please refer to the QIAgility Unpacking and Installation Instructions or to the QIAgility User Manual, chapter 4.4.1.

Important
: It is not possible to install both QIAgility Setup Manager Software and QIAgility Operating Software 4.17.1 on the same computer. Please uninstall QIAgility Operating Software 4.17.1 before installing QIAgility Setup Manager Software. For more information, contact QIAGEN Technical Services.

Note
: QIAgility Operating Software is not compatible with CAS-1200 instruments. Download the latest CAS-1200 operating software.
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Instrument operating software designed for flexible use in various applications. Use this software for all applications other than gene expression. For gene expression applications, we recommend the QIAgility Setup Manager software. The application spectrum of the QIAgility Setup Manager software will be expanded with future development of plug-ins for genotyping, pathogen testing, veterinary testing and food testing.

Important
: It is not possible to install both QIAgility Operating Software 4.17.1 and QIAgility Setup Manager Software on the same computer. Please uninstall QIAgility Setup Manager Software before installing QIAgility Operating Software 4.17.1. For more information, contact QIAGEN Technical Services.

Note
: QIAgility Operating Software is not compatible with CAS-1200 instruments. Download the latest CAS-1200 operating software.
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