For isolation of up to 15 µg per well total cellular DNA from plant tissue

  • Pure DNA, free from contaminants and enzyme inhibitors
  • Rapid purification of ready-to-use DNA
  • No organic extraction, no ethanol precipitation

The DNeasy 96 Plant Kit provides fast and easy silica-based DNA purification in a convenient 96-well plate format. Typical yields are 1–15 µg of high-quality DNA, depending on the plant species of the samples used.

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DNeasy 96 Plant Kit (6)
For 6 x 96 DNA minipreps: 6 DNeasy 96 Plates, Buffers, Reagents, RNase A, S-Blocks, Collection Microtubes (1.2 ml), Caps, AirPore Tape Sheets
69181
$1,713.00
The DNeasy 96 Plant Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
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DNeasy Plant procedure.|Reproducible DNA yield and quality.|Reliable PCR.|DNA purity from pine needles.|DNeasy 96 Plant plate.|Uniformity of DNA yields from 96 wheat samples.|
|Genomic DNA was purified from different plant species following disruption in liquid nitrogen. DNA was eluted in 2 x 100 µl Buffer AE. Eluates (10 µl) were subjected to gel electrophoresis. Average yields are given under the plant names. M: lambda–HindIII.|Genomic DNA purified from different plant species was amplified by PCR. Eluate (5 µl) was used as template, and 30 µl of each PCR product were loaded per lane. M: markers.|Spectrophotometric scans (220–320 nm) of DNA isolated from pine needles using the method of Dellaporta, CTAB, or the DNeasy Plant Mini Kit. Pure DNA typically shows a symmetrical peak at 260 nm and a smooth profile. Polysaccharides and other secondary metabolites, often copurified with plant DNA isolated using traditional methods, can interfere with OD readings (A260/A280), leading to errors in determination of concentration and purity.||Ninety-six leaf samples were collected from a batch of wheat plants grown under identical conditions. Samples (50 mg) were disrupted in liquid nitrogen and DNA was isolated using the DNeasy 96 Plant procedure.
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Performance

The DNeasy 96 Plant Kit provides high-throughput isolation of high-quality DNA from 96 or 192 samples of plant leaf tissue in less than 2 hours. The simple 96-well procedure provides highly reproducible yields of total cellular DNA (see figures "Uniform DNA yields" and "Reproducible DNA yield and quality"). The typical yield is 1–15 µg per 50 mg plant leaf tissue, with an elution volume of 100–200 µl. DNA yields vary between species depending on genome size, ploidy, cell number, and age of tissue sample.

DNeasy purified DNA is sized up to 40 kb, with fragments of 20–25 kb predominating. Polysaccharides, proteins, and enzyme inhibitors are efficiently removed. Absorbance scans of DNeasy purified DNA show a symmetrical peak at 260 nm, confirming that the DNA is free of impurities, including enzyme inhibitors.  (see figures "DNA purity from oak leaves", "DNA purity from pine needles", and "Reproducible DNA yield and quality"). Purified DNA is ready for use in all downstream applications (see figure "Reliable PCR").

High-throughput sample disruption

For rapid, efficient disruption of up to 192 plant tissue samples in just 2–4 minutes, QIAGEN offers the first commercially available high-throughput disruption system — the TissueLyser. The TissueLyser uniformly disrupts samples allowing reproducible, high-quality results in downstream applications such as genomic DNA isolation from plant tissues.

Principle

The DNeasy 96 Plant Kit uses advanced silica-membrane technology and simple spin procedures to isolate highly pure total cellular DNA from plant tissues and cells or fungi. The streamlined DNeasy 96 procedure and convenient 96-well format eliminate tedious labeling, one-at-a-time pipetting, and DNA resuspension steps, and help minimize cross-contamination. DNeasy technology replaces cumbersome DNA isolation procedures such as cetyltrimethylammonium bromide (CTAB), phenol, or chloroform extraction. Using the DNeasy procedure, alcohol precipitation is not necessary — purified DNA is ready for immediate use. DNeasy sample preparation technology is fully licensed.

Procedure

Samples are first mechanically disrupted and then chemically lysed (see flowchart "DNeasy Plant procedure"). RNA is removed by RNase digestion during lysis. Cell debries, precipitated proteins and polysaccharides are removed by centrifugation. Buffering conditions are adjusted, and the lysates are loaded onto the DNAeasy Plant 96-well plate (see figure "DNeasy 96 Plant plate"). During a brief spin, DNA selectively binds to the silica membrane while contaminants pass through. Remaining contaminants and enzyme inhibitors are removed in one efficient wash step. Pure DNA is then eluted in water or low-salt buffer, ready for use. Processing of DNeasy 96-well plates requires use of the QIAGEN 96-Well-Plate Centrifugation System.

Applications

The DNAeasy 96 Plant Kit provides purification of DNA from plant tissue, including:

Plant cells
Plant tissues
Fungi
Feature
Specifications
Applications PCR, real-time PCR, blotting
Elution volume 100–200 µl
Format 96-well plate
Main sample type Plant samples
Processing Manual
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein DNA
Sample amount 50 mg
Technology Silica technology
Time per run or per prep <2 hours (192 samples)
Yield 1–15 µg

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Kit Handbooks
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The names of some of the buffers supplied with the kit have been changed. Please note that the composition of all the buffers remains unchanged.
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