For low- to medium-throughput sample disruption for molecular analysis
Simultaneous disruption of up to 12 samples
Compact instrument with small footprint
Coolable adapter to prevent biomolecule degradation
Reproducible results with all sample types
Compatible with all laboratory workflows
The TissueLyser LT is a small bead mill which provides fast, effective disruption of up to 12 samples at the same time. This throughput matches that of the QIAcube, which automates sample preparation using trusted QIAGEN spin-column kits. Simultaneous disruption and homogenization is achieved through high-speed shaking of samples in 2 ml microcentrifuge tubes with stainless steel or glass beads. The TissueLyser LT must be used in combination with the coolable TissueLyser LT Adapter, which holds tubes during the disruption process. Additional accessories include beads and bead dispensers.
Explore the virtual demo to learn more about the TissueLyser LT.
Compact bead mill, 100–240 V AC, 50–60 Hz; requires the TissueLyser LT Adapter, 12-Tube (available separately)
The TissueLyser LT is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Effective tissue disruption.
Various rat tissues were disrupted using the TissueLyser LT or TissueLyser II. [A] DNA was purified from 25 mg samples on the QIAcube using the DNeasy Blood & Tissue Kit. DNA yields were determined using a spectrophotometer. [B] RNA was purified from 20 mg samples on the QIAcube using the RNeasy Fibrous Tissue Mini Kit (skin, heart, and lung) or RNeasy Lipid Tissue Mini Kit (brain). RNA yields were determined using a spectrophotometer.
Intact RNA from plant tissues.
Various plant tissues (100 mg each) were disrupted in duplicate in precooled adapters using the TissueLyser LT. RNA was purified using the RNeasy Plant Mini Kit and then analyzed on an agarose gel. The sharp ribosomal RNA bands indicate purification of intact RNA.
Pure RNA with high RIN values.
Rat heart and lung (20 mg each) were disrupted using the TissueLyser LT. RNA was purified on the QIAcube using the RNeasy Fibrous Tissue Mini Kit and then analyzed on the Agilent 2100 Bioanalyzer. The RIN values were 8.3 (heart) and 9 (lung).
Genotyping, gene expression, and proteomics applications demand effective disruption of biological samples to ensure high yields of DNA, RNA, and protein. The TissueLyser LT system delivers thorough and rapid disruption of samples to fully release biomolecules, and also simultaneously homogenizes samples to facilitate subsequent purification procedures using QIAGEN kits (see table "QIAGEN purification kits compatible with QIAGEN disruption systems").
The TissueLyser LT is an integral part of QIAGEN's complete solution for sample management — from sample collection to purification and analysis of DNA, RNA, and protein. The TissueLyser LT is fully compatible with QIAGEN manual sample preparation kits (see table "QIAGEN purification kits compatible with QIAGEN disruption systems"), and also complements QIAGEN's range of automated solutions for medium-throughput sample preparation and analysis (see table "QIAGEN medium-throughput automation"). These include the QIAcube, which automates sample purification using QIAGEN spin-column kits, and the QIAxcel, which automates multicapillary gel electrophoresis of DNA and RNA.
QIAGEN purification kits compatible with QIAGEN disruption systems
RNeasy Plus Kits
RNeasy Protect Kits
RNeasy Fibrous Tissue Kits
All types of tissue
RNeasy Lipid Tissue Kits
RNeasy Universal Tissue Kits
RNeasy Plant Mini Kit
RNeasy Protect Bacteria Kits
All types of tissue
QIAamp DNA Kits
DNeasy Blood & Tissue Kits
Qproteome Mammalian Protein Prep Kit
PhosphoProtein Purification Kit
Qproteome Glycoprotein Kits
DNA and RNA
AllPrep DNA/RNA Kits
DNA, RNA, and protein
AllPrep DNA/RNA/Protein Mini Kit
QIAGEN medium-throughput automation
Purification of DNA, RNA, and protein
Up to 12 samples per run
Purification of DNA and RNA from human samples
Up to 6 samples per run
EZ1 Advanced XL
Purification of DNA and RNA from human samples
Up to 14 samples per run
Electophoretic analysis of DNA fragments and RNA
Up to 96 samples per run
Up to 384 samples per run
Real-time PCR and high-resolution melting (HRM) analyses
Up to 100 samples per run
Methylation and mutation analyses
Up to 24 samples per run
The TissueLyser LT works in combination with the TissueLyser LT Adapter. Up to twelve 2 ml tubes, each containing a sample and a bead, are loaded into the adapter, which is securely fastened to the piston of the TissueLyser LT. The piston moves up and down rapidly, leading to simultaneous disruption and homogenization of the samples due to the beating and grinding action of the beads.
The TissueLyser LT has a small footprint of 15 cm x 27 cm, allowing installation in any laboratory. As each sample is safely sealed within its own tube, the TissueLyser LT is able to disrupt multiple samples without any risk of cross-contamination. If disrupting fresh or frozen samples, the TissueLyser LT Adapter can be precooled on dry ice to prevent degradation of nucleic acids and proteins (see figures "High-quality DNA from plant tissues" and "Intact RNA from plant tissues"). Tissues stored in Allprotect Tissue Reagent (to stabilize DNA, RNA, and protein) or in RNAlater RNA Stabilization Reagent (to stabilize RNA) require no precooling of the adapter.
The ability to process up to 12 samples per run makes the TissueLyser LT the ideal front-end solution to access biological information for genomics, transcriptomics, and proteomics applications. The TissueLyser LT enables fast and uniform disruption of animal and human tissues, plant tissues, bacteria, and yeast in the TissueLyser LT Adapter, which holds up to twelve 2 ml sample tubes. Sample purification can be performed manually, or can be automated using the QIAcube, QIAsymphony SP, QIAxtractor, EZ1 Advanced, EZ1 Advanced XL, or BioRobot, or BioSprint instruments.
Weight: Approx. 7 kg, Width: 150 mm, Depth: 270 mm, Height: 280 mm, The power supply of the TissueLyser LT is compatible with voltages of 100–240 V
High-speed shaking of samples in 2 ml microcentrifuge tubes with stainless steel or glass beads
Simultaneous disruption of up to 12 samples. Compact instrument with small footprint. Coolable adapter to prevent biomolecule degradation. Reproducible results with all sample types. Compatible with all laboratory workflows.
For 100 x 50 µl reactions: QIAGEN OneStep RT-PCR Enzyme Mix (1 x 200 µl), 5x QIAGEN OneStep RT-PCR Buffer (1 x 1 ml), dNTP Mix (1 x 200 µl, 10 mM each), 5x Q-Solution (1 x 2 ml), RNase-Free Water (2 x 1.9 ml)