QIAcuity Digital PCR System
For nanoplate-based digital PCR applications
- Fully integrated system
- Scalable format (1-, 4- and 8-plate instruments)
- Advanced multiplexing capabilities (up to 5 plex)
- Flexible sample throughput
- Comprehensive results in under 2 hours
The QIAcuity Digital PCR System is designed to deliver precise and multiplexed quantification results for mutation detection, copy number variation (CNV), gene expression studies, gene-editing analysis, and many more. This nanoplate-based system seamlessly integrates a standard dPCR workflow of partitioning, thermocycling and imaging into a walk-away automated platform with minimal hands-on time. The system is used in conjunction with the QIAcuity Nanoplates and Accessories. Explore the virtual demo to learn more about QIAcuity.
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QIAcuity One, 2plex Device
One-plate digital PCR instrument for detecting up to 2 fluorescent dyes, roller, USB flash memory and QIAcuity Software Suite: includes 1 preventive maintenance visit. 1 year warranty on labor, travel, and parts also included.
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911001
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QIAcuity One, 2plex System
One-plate digital PCR instrument for detecting up to 2 fluorescent dyes, roller, USB flash memory and QIAcuity Software Suite: includes installation, training, and 1 preventive maintenance visit. 1 year warranty on labor, travel, and parts also included.
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911002
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QIAcuity One, 2plex System FUL-2
One-plate digital PCR instrument for detecting up to 2 fluorescent dyes, roller, USB flash memory and QIAcuity Software Suite: includes installation, training, Full Agreement for 2 years with a 2 business day response time, and 2 preventive maintenance visits.
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911006
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QIAcuity One, 5plex Device
One-plate digital PCR instrument for detecting up to 5 fluorescent dyes, roller, USB flash memory and QIAcuity Software Suite: includes 1 preventive maintenance visit. 1 year warranty on labor, travel, and parts also included.
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911021
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QIAcuity One, 5plex System
One-plate digital PCR instrument for detecting up to 5 fluorescent dyes, roller, USB flash memory and QIAcuity Software Suite: includes installation, training, and 1 preventive maintenance visit. 1 year warranty on labor, travel, and parts also included.
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911022
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QIAcuity One, 5plex System FUL-2
One-plate digital PCR instrument for detecting up to 5 fluorescent dyes, roller, USB flash memory and QIAcuity Software Suite: includes installation, training, Full Agreement for 2 years with a 2 business day response time, and 2 preventive maintenance visits.
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911026
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QIAcuity Four Platform System
Four-plate digital PCR instrument for detecting up to 5 fluorescent dyes, notebook computer, barcode scanner, roller, USB flash memory and QIAcuity Software Suite: includes installation, training, and 1 preventive maintenance visit.1 year warranty on labor, travel, and parts.
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911042
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QIAcuity Four Platform System FUL-2
Four-plate digital PCR instrument for detecting up to 5 fluorescent dyes, notebook computer, barcode scanner, roller, USB flash memory and QIAcuity Software Suite: includes installation, training, Full Agreement for 2 years with a 2 business day response time, and 2 preventive maintenance visits.
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911046
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QIAcuity Four Platform System PRM-3
Four-plate digital PCR instrument for detecting up to 5 fluorescent dyes, notebook computer, barcode scanner, roller, USB flash memory and QIAcuity Software Suite: includes installation, training, Premium Agreement for 3 years with a next business day response time, and 3 preventive maintenance visits.
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911049
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QIAcuity Eight Platform System
Eight-plate digital PCR instrument for detecting up to 5 fluorescent dyes, notebook computer, barcode scanner, nanoplate roller, USB flash memory and QIAcuity Software Suite: includes installation, training, and 1 preventive maintenance visit. 1 year warranty on labor, travel, and parts.
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911052
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QIAcuity Eight Platform System FUL-2
Eight-plate digital PCR instrument for detecting up to 5 fluorescent dyes, notebook computer, barcode scanner, nanoplate roller, USB flash memory and QIAcuity Software Suite: includes installation, training, Full Agreement for 2 years with a 2 business day response time, and 2 preventive maintenance visits.
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911056
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QIAcuity Eight Platform System PRM-3
Eight-plate digital PCR instrument for detecting up to 5 fluorescent dyes, notebook computer, barcode scanner, nanoplate roller, USB flash memory and QIAcuity Software Suite: includes installation, training, Premium Agreement for 3 years with a next business day response time, and 3 preventive maintenance visits.
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911059
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QIAcuity One Installation & Training
Installation of instrument hardware and system software. Includes application training for hands-on experience in operating the instrument and qualifying laboratory staff. Labor, travel, and installation kit (if applicable) included.
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9245352
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QIAcuity Four Installation & Training
Installation of instrument hardware and system software. Includes application training for hands-on experience in operating the instrument and qualifying laboratory staff. Labor, travel, and installation kit (if applicable) included.
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9245353
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QIAcuity Eight Installation & Training
This service combines Installation with Applications Training to help customers gain hands-on experience of hardware, software, applications using QIAGEN platforms and facilitates professional qualifications of laboratory staff.
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9245354
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QIAcuity One Preventive Subscription
One on-site preventive maintenance (PM), including travel, labor, and PM parts. Includes a 10% discount on repair services during Preventive Subscription period and documentation in a GMP/GLP-compliant visit report.
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9245355
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QIAcuity Four Preventive Subscription
One on-site preventive maintenance (PM), including travel, labor, and PM parts. Includes a 10% discount on repair services during Preventive Subscription period and documentation in a GMP/GLP-compliant visit report.
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9245356
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QIAcuity Eight Preventive Subscription
One on-site preventive maintenance (PM), including travel, labor, and PM parts. Includes a 10% discount on repair services during Preventive Subscription period and documentation in a GMP/GLP-compliant visit report.
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9245357
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QIAcuity One 2plex Core Agreement
One on-site repair service and one-on-site preventive maintenance including travel, labor, and spare parts for a period of one year. Response time of five business days. Includes a 10% discount on additional repair service during agreement period.
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9245358
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QIAcuity One 5plex Core Agreement
One on-site repair service and one-on-site preventive maintenance including travel, labor, and spare parts for a period of one year. Response time of five business days. Includes a 10% discount on additional repair service during agreement period.
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9245359
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QIAcuity Four Core Agreement
One on-site repair service and one-on-site preventive maintenance including travel, labor, and spare parts for a period of one year. Response time of five business days. Includes a 10% discount on additional repair service during agreement period.
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9245360
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QIAcuity Eight Core Agreement
One on-site repair service and one-on-site preventive maintenance including travel, labor, and spare parts for a period of one year. Response time of five business days. Includes a 10% discount on additional repair service during agreement period.
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9245361
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QIAcuity One 2plex Basic Agreement
On-site QIAcuity One 2plex instrument repair, including travel, labor, and parts, for a period of one year. Response time of five business days. Includes one Preventive Maintenance or Inspection Service during the Basic Agreement period.
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9245401
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QIAcuity One 5plex Basic Agreement
On-site QIAcuity One 5plex instrument repair, including travel, labor, and parts, for a period of one year. Response time of five business days. Includes one Preventive Maintenance or Inspection Service during the Basic Agreement period.
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9245402
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QIAcuity Four Basic Agreement
On-site QIAcuity Four instrument repair, including travel, labor, and parts, for a period of one year. Response time of five business days. Includes one preventive maintenance during the Basic Agreement period.
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9245403
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QIAcuity Eight Basic Agreement
On-site QIAcuity Eight instrument repair, including travel, labor, and parts, for a period of one year. Response time of five business days. Includes one preventive maintenance during the Basic Agreement period.
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9245404
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QIAcuity One 2plex Full Agreement
On-site QIAcuity One 2plex instrument repair, including travel, labor, and parts, for a period of one year. Response time of two business days. Includes one preventive maintenance during the Full Agreement period.
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9245362
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QIAcuity One 5plex Full Agreement
On-site QIAcuity One 5plex instrument repair, including travel, labor, and parts, for a period of one year. Response time of two business days. Includes one preventive maintenance during the Full Agreement period.
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9245363
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QIAcuity Four Full Agreement
On-site QIAcuity Four instrument repair, including travel, labor, and parts, for a period of one year. Response time of two business days. Includes one preventive maintenance during the Full Agreement period.
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9245364
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QIAcuity Eight Full Agreement
On-site QIAcuity Eight instrument repair, including travel, labor, and parts, for a period of one year. Response time of two business days. Includes one preventive maintenance during the Full Agreement period.
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9245365
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The QIAcuity is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease. Therefore, the performance characteristics of the product for clinical use (i.e., diagnostic, prognostic, therapeutic or blood banking) is unknown.
Performance
The QIAcuity Digital PCR System makes absolute quantification accessible and affordable for all laboratories. The walk-away automation integrates and streamlines the entire digital PCR workflow of partitioning, thermocycling and imaging into a single instrument with minimal hands-on time. It is also easy to adapt your current qPCR assays to the QIAcuity Digital PCR System. No change in plate handling is required when coming from qPCR, assuring fast assay setup and quick results in under 2 hours. QIAcuity Instruments – features and specifications | Feature | QIAcuity One | QIAcuity Four | QIAcuity Eight | | Plates processed | 1 | 4 | 8 | | Detection channels (multiplexing) | 2 or 5 | 5 | 5 | | Thermocycler(s) | 1 | 1 | 2 | | Time to result | Under 2 h | First plate under 2 h Every ~60 min a following plate | First plate under 2 h Every ~30 min a following plate | | Throughput (samples processed in a work day) | Up to 384 (96-well) Up to 96 (24-well) | Up to 672 (96-well) Up to 168 (24-well) | Up to 1248 (96-well) Up to 312 (24-well) |
Principle
In just 3 simple steps, you can have the dPCR result you want in under 2 hours: pipette and load, run the experiment, analyze results. The principle of the dPCR reaction in the nanoplates is described here.
Procedure
Just like in qPCR experiments, sample preparation includes the transfer of master mix, probes and primers to a 96- or 24-well nanoplate, followed by the addition of samples. The system integrates partitioning, thermocycling and imaging into a single fully automated instrument that takes users from the sample to result in under 2 hours. One can perform analysis on the Software Suite, providing the concentration in copies per microliter of your target sequence as well as for quality control such as positive samples or NTC. This analysis can also be extended to remote computers within the same local area network (LAN).
Applications
The QIAcuity instruments, together with the QIAcuity Nanoplates and the QIAcuity PCR kits, enable digital PCR applications, including: - Rare mutation detection
- Copy number variation analysis
- Gene expression analysis
- Pathogen detection
- Genotyping
- miRNA research
Services
Protect your instrument with diverse service solutions from QIAGEN. Learn more about a specific service agreement to match your needs.
Software
The QIAcuity Software Suite provided with the instrument and installed on a separate computer controls one or multiple QIAcuity instruments, either connected directly to one instrument or using an existing local area network (LAN). Using the QIAcuity Software Suite, digital PCR experiments, samples and reaction mixes can be defined, assigned to Nanoplates, and transferred to the QIAcuity instrument. After the run, data can be analyzed, reports can be created and data can be exported for external analysis. The software provides multiple template functionalities to make repeating plate layouts or plate run parameters easily accessible, further transforming your digital PCR experience. When integrated into a local area network, the computer is hosting the QIAcuity Software Suite function as a server that is accessible via LAN to other computers serving as clients. This enables multiple users to access the software from other rooms or offices and analyze data via a standard browser without the need to install the software on multiple computers or access and exchange data via internet connections.
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Version 1.1.3
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Version 1.0
The QIAcuity Control Software is an integral part of the QIAcuity instrument. It offers a GUI (graphical user interface) for basic functionalities such as plate setup, changing the order of plates to be processed, and monitoring the status of runs in real time. After a run is completed, the data are stored on the instrument’s memory and are sent to the connected QIAcuity Software Suite for analysis.
This new version of the control software includes system stability and performance reliability improvements. We highly recommend to update the control software of your QIAcuity instrument.
Note: The installation requires approximately 90 minutes.
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For Version 1.0
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For Version 1.2
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Version 1.2
The QIAcuity Software Suite 1.2 is designed to be installed on a Windows PC that is connected to one or more QIAcuity instruments. The QIAcuity Software Suite enables the user to set up plates, analyze results, and monitor the status of runs in real time. For this configuration, the QIAcuity instrument needs to be connected to a network through Ethernet. Alternatively, a direct cable connection between the QIAcuity and the notebook where the QIAcuity Software Suite is running needs to be established. When connected to a network, up to 10 users may access the QIAcuity Software Suite via a browser installed on the client PC (Windows or Mac).
The following browsers are supported in the QIAcuity Software Suite:
-Mozilla Firefox (version 64.0.2 or higher)
-Microsoft Edge (version 44.17763.1.0 or higher)
-Google Chrome (version 71.0.3578.98 or higher)
The new QIAcuity Software Suite 1.2 offers a functionality that enables users of the QIAcuity Software 1.1.3 to upgrade to the new version while keeping the library of previously stored plate runs.
Note: If you have exported plates from QIAcuity Software Suite 1.1.3 that you would like to import and use in QIAcuity Software Suite 1.2, you will need to import the plates before upgrading from version 1.1.3 to version 1.2. You may then export the plates again. Future software version starting from QIAcuity Software Suite 2.0 will facilitate import of plates from previous QIAcuity Software Suite versions.
The new improvements are as follows:
-Support for the Nanoplate 8.5k 24-well
-Hyperwell functionality to combine several wells to one combined well for analysis
-Automated plate archiving functionality
-Functionality to show the number of single/double positives in 2D scatterplots
-VPF (Volume Precision Factor) to further improve concentration calculation (see related resources)
-Additional improvements for stabilization and troubleshooting
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Version 3.0
The Volume Precision Factor (VPF) offers a unique feature to secure precision of concentration results obtained from a QIAcuity dPCR run.
In general, Nanoplates provide partitions of fixed sizes that enable a very precise way of sample concentration calculation. Potential variation of partition sizes in Nanoplate batches, caused by different microstructure molding forms, can be addressed by applying the batch specific VPF. Furthermore, the VPF includes well-specific volume information and therefore further increases precision of concentration calculation in each well of the Nanoplates.
After downloading and updating the VPF file within the QIAcuity Software Suite, the VPF is applied automatically to the analysis of a corresponding Nanoplate batch. The VPF file includes information from all available microstructure molding forms and connected Nanoplate batches. It will be stored on the PC where the QIAcuity Software Suite is installed.
Required QIAcuity Software Suite version: Version 1.2 or higher.
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Version 2.0
The QIAcuity Control Software is an integral part of the QIAcuity instrument. It offers a GUI (graphical user interface) for basic functionalities such as plate setup, changing the order of plates to be processed, and monitoring the status of runs in real time. After a run is completed, the data are stored on the instrument’s memory and are sent to the connected QIAcuity Software Suite for analysis
Besides system stability and performance reliability improvements, this new version of the control software offers advanced user management and audit trail functionalities. Together with the QIAcuity Software Suite 2.0, the QIAcuity systems now support 21 CFR Part 11 regulations for users working under GMP.
Detailed information is available in the Release Note, which can also be downloaded under section “Operating Software”.
Note: Upgrading the QIAcuity Control Software to version 2.0 will require upgrading the QIAcuity Software Suite to version 2.0.
Important: Please follow the instructions provided in the QIAcuity User Manual for software version 2.0. It is strongly recommended to update the QIAcuity Software Suite first before proceeding with the QIAcuity Control Software!
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For Version 2.0
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For Version 2
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Version 2.0
The QIAcuity Software Suite 2.0 is designed to be installed on a Windows PC that is connected to one or more QIAcuity instruments. The QIAcuity Software Suite enables the user to set up plates, analyze results, and monitor the status of runs in real time. For this configuration, the QIAcuity instrument needs to be connected to a network through Ethernet. Alternatively, a direct cable connection between the QIAcuity and the notebook where the QIAcuity Software Suite is running needs to be established. When connected to a network, up to 10 users may access the QIAcuity Software Suite via a browser installed on the client PC (Windows or Mac).
This new software version now supports 21 CFR Part 11 regulations for users working under GMP. This includes the following:
-Advanced User Management
-Audit Trail
-Signatures
Additional changes compared with the previous software version include the following:
-Usability improvements
-Improved dust detection
-Hyperwell expansion to CNV and GEX analysis
-CSV export functionality of multiple positive partitions for linkage analysis, drop-off assays, etc.
-Raw data export
Detailed information is available in the Release Note, which can also be downloaded under section “Operating Software”.
Important: A direct update of the Software Suite from version 1.1.3 to 2.0.20 is not possible. Please follow the instructions in the User Manual on how to update to version 1.2.18 first before updating the Software Suite to version 2.0.20.
Before you start upgrading to version 1.1.3, please make sure that all plates are imported and visible in the plate overview of the QIAcuity Software Suite.
Caution: Not following the instructions may result in a loss of your previous plate data!
Please contact QIAGEN Technical Services if you are unsure and require technical support.
SHA1 checksum: C60C4EEC349DF1C037796016D32A93EEC19B54A0
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For use with QIAcuity systems
July 2021
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User manual for QIAcuity instruments and QIAcuity Software 1.2
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User manual for QIAcuity instruments and QIAcuity Software 2.0
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Network connection of QIAcuity instruments
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For QIAcuity Software Suite 1.2: Updating VPF for archived or exported plates
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For QIAcuity User Manual – Updated QIAcuity Nanoplate Partition Volumes
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Here we compared the performance of qPCR and the nanoplate dPCR techniques. The digital PCR method on the QIAcuity significantly improved precision when measuring copy number states and sensitivity of mutation detection through absolute quantification and reduced standard error. This is advantageous in various applications, including copy number variation analysis, small fold-change and rare mutation detection.
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