PyroMark Q96 MD Accessories and Reagents

For optimal sample preparation and Pyrosequencing analysis using PyroMark Q96 MD

Reliable quantification of genetic or epigenetic DNA modifications
Solutions for quick and easy sample handling for successful results
Dedicated products for each platform and each step in the workflow
High-quality optimized reagents enable reliable Pyrosequencing results

The PyroMark Q96 MD uses proven Pyrosequencing technology for real-time, sequence-based detection and quantification for genetic analysis and epigenetic methylation studies. PyroMark Q96 MD Accessories and Reagents are high-quality reagents and products that simplify and streamline the Pyrosequencing workflow and enable successful results. PyroMark Q96 MD Accessories and Reagents are designed specifically for use with PyroMark Q96 MD instruments.

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製品の詳細
関連資料

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製品名	Cat. no.	List price:
PyroMark Annealing Buffer For annealing of sequencing primer to DNA template	979009	¥10,000
PyroMark Binding Buffer (200 ml) For binding of biotinylated PCR product to Sepharose beads	979006	¥10,000
PyroMark Control Oligo For installation check of system	979203	¥40,500
PyroMark CpG Assay (200) PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (200 reactions; tube format)	Varies	¥20,500	カートに追加
PyroMark CpG Assay 96 well (200) PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (200 reactions; plate format); minimum order of 24 assays per plate	Varies	¥19,500	カートに追加
PyroMark CpG Assay 96 well (50) PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (50 reactions; plate format); minimum order of 24 assays per plate	Varies	¥12,000	カートに追加
PyroMark Custom Assay (200) User-designed PCR and sequencing primers for Pyrosequencing analysis (200 reactions; tube format)	Varies	¥20,500	カートに追加
PyroMark Denaturation Sol. (500 ml) For denaturation of double-stranded PCR product into single-stranded DNA template	979007	¥10,000
PyroMark Gold Q96 CDT Reagents (6 x 96) For performing Pyrosequencing reactions on the PyroMark Q96 MD in combination with the capillary dispensing tips (CDT)	972824	¥56,000
PyroMark Gold Q96 Reagents (5 x 96) For performing Pyrosequencing reactions on the PyroMark Q96 ID (5 x 96) and PyroMark Q96 MD (15 x 96)	972804	¥98,500
PyroMark Gold Q96 Reagents (50 x 96) For performing Pyrosequencing reactions on the PyroMark Q96 MD	972807	¥295,000
PyroMark OneStep RT-PCR Kit (200) For 200 reactions: QIAGEN OneStep RT-PCR Enzyme Mix, optimized QIAGEN OneStep RT-PCR Buffer, 10x CoralLoad Concentrate, 5x Q-Solution, dNTP Mix, and RNase-Free Water	978803	¥71,000
PyroMark OneStep RT-PCR Kit (50) For 50 reactions: QIAGEN OneStep RT-PCR Enzyme Mix, optimized QIAGEN OneStep RT-PCR Buffer, 10x CoralLoad Concentrate, 5x Q-Solution, dNTP Mix, and RNase-Free Water	978801	¥24,500
PyroMark PCR Kit (200) For 200 reactions: 2x PyroMark PCR Master Mix (includes HotStarTaq DNA Polymerase and optimized PyroMark Reaction Buffer containing 3 mM MgCl₂ and dNTPs), 10x CoralLoad Concentrate, 5x Q-Solution, 25 mM MgCl₂, and RNase-Free Water.	978703	¥38,500
PyroMark PCR Kit (800) For 800 reactions: 2x PyroMark PCR Master Mix (includes HotStarTaq DNA Polymerase and optimized PyroMark Reaction Buffer containing 3 mM MgCl₂ and dNTPs), 10x CoralLoad Concentrate, 5x Q-Solution, 25 mM MgCl₂, and RNase-Free Water	978705	¥128,000
PyroMark Q96 HS Capillary Tip Holder Reusable holder for tips, nucleotide dispensing tips, and reagent dispensing tips, for use with PyroMark Q96 MD	9019076	Inquire
PyroMark Q96 HS Dispensing Tip Holder Reusable holder for tips, nucleotide dispensing tips, and reagent dispensing tips, for use with PyroMark Q96 MD	9019075	Inquire
PyroMark Q96 HS Nucleotide Tip (8) Reusable tips (8 in each package); for dispensing nucleotides (NDTs); for use with PyroMark Q96 MD	979103	Inquire
PyroMark Q96 HS Sample Prep Thermoplate Holder for placement of PyroMark Q96 Plate on heating block during annealing step prior to PyroMark Q96 MD analysis	9019071	Inquire
PyroMark Q96 HS Tip Holder Box Box for dust-free and safe storage of tips in between runs. For use with PyroMark Q96 MD	9019074	¥7,800
PyroMark Q96 Vacuum Prep Trough (5) Reusable plastic troughs for use with all PyroMark Q96 Vacuum Workstations	979011	¥6,100
PyroMark Q96 Vacuum Workstation (110 V) Vacuum workstation for preparing 96 samples in parallel from PCR product to single-stranded template	9001528	Inquire
PyroMark Vacuum Prep Filter Probe (100) Reusable filter probes for PyroMark Vacuum Workstation Q96 and Q24	979010	¥40,500
PyroMark Wash Buffer (conc., 200 ml) For use with the PyroMark Q24 Vacuum Workstation and PyroMark Q96 Vacuum Workstation to wash and neutralize the immobilized DNA	979008	¥10,000

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PyroMark Q96 MD Accessories and Reagents は分子生物学実験用です。疾病の診断、治療または予防の目的には使用することはできません。

アプリケーション

PyroMark Q96 MD Accessories and Reagents enable Pyrosequencing analysis with the PyroMark Q96 MD instrument for applications such as:

HRM
Methylation analysis
Mutation analysis
SNP analysis

FAQ

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	What concentration should be used for the sequencing primer in pyrosequencing? FAQ ID -2826	表示
	Can I order the nucleotides from PyroMark Gold Reagents separately? FAQ ID -2827	表示
	Which purity grade is recommended for pyrosequencing primers? FAQ ID -2832	表示
	How many times can vacuum troughs be re-used with the PyroMark Vacuum Preparation Stations? FAQ ID -2848	表示
	Where can I order the Streptavidin Sepharose beads for pyrosequencing? FAQ ID -2850	表示
	How many nucleotides of a homopolymer can be resolved in pyrosequencing? FAQ ID -2871	表示
	Can unused wells in a pyrosequencing plate be used in the next run? FAQ ID -2872	表示
	What is the reason for signals ceasing in the middle of a pyrosequencing run? FAQ ID -2875	表示
	How do I reduce background peaks in the pyrosequencing pyrogram? FAQ ID -2877	表示
	How do I prevent a drifting baseline in my pyrosequencing pyrogram? FAQ ID -2878	表示
	What is the reason for a high substrate peak in the pyrosequencing pyrogram? FAQ ID -2879	表示
	What is the reason for split peaks appearing in between dispensations on my pyrosequencing pyrogram? FAQ ID -2881	表示
	How accurate and reliable is PyroMark Q24 in mutation analysis? FAQ ID -2094	表示
	Which kits can be used in combination with the PyroMark CpG Assays and PyroMark Custom Assays? FAQ ID -2822	表示
	What kind of shaker should be used for the pyrosequencing binding step? FAQ ID -2837	表示
	Which heating block is recommended for the pyrosequencing annealing step? FAQ ID -2838	表示
	Can PyroMark Gold reagents be vortexed? FAQ ID -2844	表示
	What is the concentration of PyroMark Control Oligo? FAQ ID -2846	表示
	What should be the single peak height for the PyroMark Control Oligo on the different PyroMark instruments? FAQ ID -2852	表示
	What is the use of the PyroMark Q24 Validation Oligo? FAQ ID -2855	表示
	How many times can the cartridges for Pyromark Q24 or PyroMark Q96 ID instruments are reused? FAQ ID -2863	表示
	What is the purpose of the unmethylated and unconverted control DNA of the EpiTect PCR Control DNA Set? FAQ ID -2007	表示
	How do I set up a PyroMark CpG Assay? FAQ ID -2814	表示
	How are the PyroMark CpG Assays shipped and stored? FAQ ID -2816	表示
	How are the PyroMark CpG Assays reconstituted? FAQ ID -2817	表示
	Does QIAGEN offer a design of Custom PyroMark CpG Assays? FAQ ID -2818	表示
	What are the features of PyroMark CpG Assays e.g. in terms of design, validation? FAQ ID -2821	表示
	Will the primer sequence for the PyroMark CpG Assay be provided? FAQ ID -2823	表示
	In which format can PyroMark CpG Assays be ordered? FAQ ID -2824	表示
	What is the recommended amplicon size for CpG assays? FAQ ID -2825	表示
	Is the CpG software included in the PyroMark instruments to study methylation status? FAQ ID -2842	表示
	What is included in a PyroMark Custom Assay? FAQ ID -2815	表示
	Which end of the PCR primer for pyrosequencing should be biotinylated? FAQ ID -2839	表示
	Will dUTP in a PCR reaction affect pyrosequencing? FAQ ID -2843	表示
	Which PyroMark Gold Q96 Reagent should be used for which instrument and application? FAQ ID -2836	表示
	What is the meaning of the abbreviations NDT and CDT and what are the differences? FAQ ID -2841	表示
	How many times can the CDTs, NDTs, and RDTs be used? FAQ ID -2865	表示
	Does the Pyromark Assay Design or application software give any cycling conditions for individual assay primers or a PCR setup pipetting scheme? FAQ ID -2862	表示

MSDS

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	Safety Data Sheets Download Safety Data Sheets for QIAGEN product components.	表示
	Safety Data Sheets Download Safety Data Sheets for QIAGEN product components.	表示

パンフレット

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	Product Profile - PyroMark Q24 Advanced	詳細を表示
	Pyrosequencing – the synergy of sequencing and quantification	詳細を表示

キットハンドブック

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	PyroMark Control Oligo Handbook For use with PyroMark Q48 Autoprep, PyroMark Q24 Advanced, PyroMark Q24, PyroMark Q96 ID and PyroMark Q96 MD systems	詳細を表示
	(EN) - PyroMark Gold Q96 Reagents Handbook For performing Pyrosequencing reactions on the PyroMark Q96 ID, PyroMark Q96 MD, and PyroMark Q96 MD Automated	詳細を表示
	(JA) - PyroMark OneStep RT-PCRプロトコールとトラブルシューティングパイロシークエンス（Pyrosequencing）解析用に至適化された感度と精度の高い1 ステップRT-PCR	詳細を表示
	(EN) - PyroMark OneStep RT-PCR Handbook	詳細を表示
	(JA) - PyroMark PCRプロトコールとトラブルシューティングパイロシークエンス（Pyrosequencing）解析に最適なDNAテンプレートのPCR増幅用	詳細を表示
	(EN) - PyroMark PCR Handbook	詳細を表示
	(EN) - PyroMark Q96 Capillary Tip Product Sheet	詳細を表示
	(EN) - PyroMark Q96 Nucleotide Tip Product Sheet	詳細を表示
	(EN) - PyroMark Q96 Reagent Tip Product Sheet	詳細を表示

追加リソース

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	(EN) - Offline Ordering Instructions for PyroMark CpG Assays	詳細を表示
	(EN) - Offline Ordering Instructions for PyroMark Custom Assays	詳細を表示

サイエンティフィック・ポスター

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	(EN) - PyroMark CpG Assays: A new tool for genome-wide methylation profiling by Pyrosequencing	詳細を表示
	Advanced DNA methylation analysis for verification of Infinium HumanMethylation450 BeadChip data Scientific poster	詳細を表示

テクニカルインフォメーション

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	(JA) - PyroMark システム	詳細を表示

User-Developed Protocols

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	Pyrosequencing® method for the forensic identification of tissue source using QIAGEN’s PyroMark® Q24 Advanced system - (EN)	詳細を表示

ホワイトペーパー

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	(EN) - Techniques to overcome bottlenecks in epigenetics research	詳細を表示

Application Notes

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	Tissue source attribution using the PyroMark Q48 Autoprep System: Sperm identification in forensic casework	詳細を表示
	Pyrosequencing – tissue source forensic identification using PyroMark Q24 Advanced A Pyrosequencing method for the forensic identification of tissue source using QIAGEN’s PyroMark Q24 Advanced system.	詳細を表示

クイックスタートプロトコール

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	(EN) - PyroMark PCR Kit Quick-Start Protocol	詳細を表示
	PyroMark Q96 Vacuum Workstation Quick-Start Guide - (EN)	詳細を表示

安全データシート

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	MSDS PyroMark Annealing Buffer
	MSDS PyroMark Binding Buffer (200 ml)
	MSDS PyroMark Control Oligo
	MSDS PyroMark CpG Assay (200)
	MSDS PyroMark CpG Assay 96 well (200)
	MSDS PyroMark CpG Assay 96 well (50)
	MSDS PyroMark Custom Assay (200)
	MSDS PyroMark Denaturation Sol. (500 ml)
	MSDS PyroMark Gold Q96 CDT Reagents (6 x 96)
	MSDS PyroMark Gold Q96 Reagents (5 x 96)
	MSDS PyroMark Gold Q96 Reagents (50 x 96)
	MSDS PyroMark OneStep RT-PCR Kit (200)
	MSDS PyroMark OneStep RT-PCR Kit (50)
	MSDS PyroMark PCR Kit (200)
	MSDS PyroMark PCR Kit (800)
	MSDS PyroMark Vacuum Prep Filter Probe (100)
	MSDS PyroMark Wash Buffer (conc., 200 ml)
	CofA

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