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What downstream applications have been tested with DNA purified using the PAXgene Blood DNA System?
FAQ ID - 3506
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Can I add bleach to the sample preparation waste?
FAQ ID - 3505
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What is the recommended range of cells for the miScript Single Cell qPCR Kit?
FAQ ID - 3565
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Can purified RNA be used with the miScript Single Cell qPCR Kit?
FAQ ID - 3566
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Can the miScript Single Cell qPCR Kit be used with plant cells?
FAQ ID - 3568
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Are any of the miScript Single Cell qPCR Kit components interchangeable with miScript II RT Kit, miScript Plant RT Kit, miScript PreAMP PCR Kit, or miScript Microfluidics PreAMP Kit components?
FAQ ID - 3569
View
Can the miScript Single Cell qPCR Kit be used to quantify piRNA expression?
FAQ ID - 3567
View
Should miScript PreAMP Primer Mixes be used in conjunction with the miScript Single Cell qPCR Kit?
FAQ ID - 3570
View
I have accidentally pipetted beads when removing the eluate after cDNA cleanup. What should I do?
FAQ ID - 3572
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Is the cDNA cleanup step in the miScript Single Cell qPCR Kit workflow absolutely required?
FAQ ID - 3571
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Is the miRNA reverse transcription control (miRTC) used in conjunction with the miScript Single Cell qPCR Kit?
FAQ ID - 3573
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Does the miScript Single Cell qPCR Kit contain controls?
FAQ ID - 3574
View
What is the amplicon size of PCR products generated with the miScript Single Cell qPCR System?
FAQ ID - 3578
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I do not see any miRNA expression for my miRNAs of interest after performing single cell miRNA expression analysis with the miScript Single Cell qPCR Kit and miScript miRNA PCR Arrays or miScript Primer Assays. What could be the cause?
FAQ ID - 3577
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When performing real-time PCR quality control associated with the miScript Single Cell qPCR Kit, is it necessary to perform control experiments for all individual samples/cells?
FAQ ID - 3576
View
What is the dissociation curve temperature of PCR products generated with the miScript Single Cell qPCR System?
FAQ ID - 3579
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When performing real-time PCR quality control associated with the miScript Single Cell qPCR Kit, is it necessary to perform control experiments for all individual samples/cells?
FAQ ID - 3575
View
Do you recommend normalizing single cell miRNA expression data to a “housekeeping” gene or miRNA?
FAQ ID - 3580
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How should data be analyzed when the miScript Single Cell qPCR Kit is used with miScript miRNA PCR Arrays or miScript Primer Assays?
FAQ ID - 3581
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What single cell isolation methods are recommended for use with the miScript Single Cell qPCR Kit?
FAQ ID - 3582
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Is clot detection possible on the QIAsymphony?
FAQ - ID 3588
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How would I provide information on the QuantiTect Primer assays in publications when the sequence is confidential?
FAQ ID - 3593
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In which tubes is clot detection possible on QIAsymphony?
FAQ ID - 3589
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Which barcodes on sample tubes are accepted by the QIAsymphony SP?
FAQ ID - 3598
View
Is it possible to measure the proteolytic activity of proteins on the surface of exosomes that were isolated with ExoEasy?
FAQ ID - 3599
View
How can I create a report/logfile on my QIAsymphony SP system?
FAQ ID - 3594
View
How can I determine if a tube is suitable for clot detection on QIAsymphony?
FAQ ID - 3590
View
Can the Rotor-Gene Q be connected to LIMS?
FAQ ID - 3596
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Can I use blood, cells or bacteria with the QIAamp Fast DNA Tissue Kit?
FAQ ID - 3587
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How should blood samples drawn into PAXgene Blood DNA Tubes (IVD) be stored?
FAQ ID - 3585
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How should frozen PAXgene Blood DNA Tubes (IVD) be thawed?
FAQ ID - 3586
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Can the REPLI-g Mitochondrial DNA Kit be used for species other than humans?
FAQ ID - 3584
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What is the expected amplicon size of the Microbial DNA qPCR Assays?
FAQ ID — 3396
View
Which Microbial qPCR Mastermix should I use?
FAQ ID — 3395
View
What are the storage conditions for the Microbial DNA qPCR products?
FAQ ID — 3394
View
3326 - Which QIAcube standard protocol might be suitable to extract RNA from saliva or from a buccal cell pellet?
View
3327 - How often should the o-ring for the pipettor tip-adapter be changed on the QIAcube?
View
3328 - Can the Sample Tubes CB and the Sample Tubes RB be used interchangeably?
View
3329 - When using REPLI-g, if the starting material is a single sorted cell, could mitochondrial DNA (mtDNA) be amplified?
View
3330 - Is there any contamination from E. coli genomic DNA in the polymerase provided with the Repli-G Single Cell Kit?
View
3332 - Is there a method that can distinguish between non-amplified DNA and REPLI-g amplified DNA?
View
3333 - When using REPLI-g, what is the purpose of the DTT added to the denaturation buffer in the cells/blood protocol?
View
What sample types can be tested on the arrays/assays?
FAQ ID — 3399
View
What is the difference between Positive PCR Control (PPC) and Microbial DNA Positive Control?
FAQ ID — 3397
View
3331 - Should the REPLI-g amplified mtDNA be cleaned up before sequencing?
View
Can I use the Microbial DNA-Free Water and Microbial qPCR Mastermix if they have been opened more than 3 times?
FAQ ID — 3398
View
What are the minimum sample requirements for Microbial DNA qPCR kits?
FAQ ID — 3393
View
View
What is the QIAxcel QX RNA Size Marker 200-6000nt?
FAQ ID - 3365
View
What is the nature of the gel in the QIAxcel RNA QC kit v2.0? Is it denaturing?
FAQ ID - 3366
View
3352 - What are the size ranges of Ni-NTA particles?
FAQ ID - 3352
View
3353 - What is the composition of the elution buffer in the Ni-NTA Fast Start Kit?
FAQ ID - 3353
View
3354 - What type and amount of resin is packed into the Ni-NTA Spin columns from the Ni-NTA Spin Kit?
FAQ ID - 3354
View
3351 - What is the upper limit for protein size that can bind to Ni-NTA agarose resin?
FAQ ID - 3351
View
3344 - What is the difference between the EpiTect Plus DNA Bisulfite kit compared to the classic kit?
FAQ - 3344
View
3341 - How are the EpiTect DNA controls produced and tested?
FAQ - 3341
View
3345 - What is the difference between the high and low concentration EpiTect Bisulfite Conversion protocol?
FAQ - 3345
View
3311 - Can QuickLyse miniprep kits be used with QIAVac 24 Plus?
View
Why does AllPrep DNA/RNA/Protein Mini kit use buffer RLT, but Allprep DNA/RNA Mini and Allprep DNA/RNA Micro kits use buffer RLT Plus? Are these buffers interchangeable?
FAQ ID - 3391
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3312 - For DirectPrep 96 miniprep, is it possible to use a half plate and save the other half for later prep?
View
3313 - What is the endotoxin level for the QIAGEN Plasmid Plus kits?
View
3316-What is the concentration of the reconstituted QuantiFast Probe Assay?
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What is the expected purity of DNA extracted from blood samples collected in PAXgene Blood DNA Tubes (IVD)?
FAQ ID - 3383
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CAn I use milk or other liquids with the DNeasy mericon Food kit? What volume would I use?
FAQ ID - 3350
View
Why does the DNeasy mericon Food Kit use a QIAquick column and Buffer PB rather than a DNeasy column and Buffer AL, which might be expected since the kit isolates genomic DNA?
FAQ ID - 3347
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3314-What is the concentration of RNase A sold separately?
View
Does salt concentration in the food matter when using the DNeasy mericon Food Kit?
FAQ ID - 3346
View
When would I use the mericon DNA Bacteria Kit vs the mericon DNA Bacteria Plus Kit?
FAQ ID - 3348
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Is it possible to stop the DNeasy tissue protocol and store the tissue lysates after digesting in buffer ATL and Proteinase K?
FAQ ID - 3362
View
Why is my 260/280 ratio low after using the DNeasy mericon Food Kit?
FAQ ID - 3349
View
Are QIAcube reagent bottles (cat. no. 990393) autoclavable? What type of plastic are they made of?
FAQ ID - 3369
View
Are there any special considerations for the collection of blood using PAXgene Blood DNA Tubes (IVD) (e.g., use of a blood collection set or discard tube, positioning of the tube, issues with short draws, etc.)?
FAQ ID - 3371
View
Are PAXgene Blood DNA Tubes (IVD) sterile?
FAQ ID - 3373
View
What is the additive in PAXgene Blood DNA Tubes (IVD)?
FAQ ID - 3372
View
What is the composition of QIAzol? What is the color of this reagent?
FAQ ID - 3355
View
What is the shelf life of PAXgene Blood DNA Tubes (IVD) prior to blood collection?
FAQ ID - 3374
View
How long can samples in PAXgene Blood DNA Tubes (IVD) be stored at room temperature?
FAQ ID - 3378
View
How should blood samples drawn into PAXgene Blood DNA Tubes (IVD) be transported?
FAQ ID - 3375
View
How long can samples in PAXgene Blood DNA Tubes (IVD) be stored at 2–8°C?
FAQ ID - 3379
View
Can blood samples be frozen in PAXgene Blood DNA Tubes (IVD)?
FAQ ID - 3377
View
Can PAXgene Blood DNA Tubes (IVD) be stored at higher than room temperature (18–25°C)?
FAQ ID - 3380
View
Which downstream applications have been tested with DNA purified from samples collected in PAXgene Blood DNA Tubes (IVD)?
FAQ ID - 3384
View
What is the composition of elution buffers used in QIAsymphony DNA Investigator kits?
FAQ ID - 3387
View
Can I buy the gDNA eliminator plates supplied in your RNeasy Plus 96 kit separately?
FAQ ID - 3389
View
Can I buy the gDNA eliminator columns supplied in your RNeasy Plus kits separately?
FAQ ID - 3390
View
3323 - Do you have a protocol for the AllPrep DNA/RNA/Protein Mini Kit on the QIAcube?
FAQ ID - 3323
View
3324 - Do you have a protocol for the QIAprep Spin M13 Kit on the QIAcube?
View
3340 - Which operating system is compatible with PyroMark IdentiFire Software?
View
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Do I have to use a blood collection set with PAXgene Blood DNA Tubes (IVD)?
FAQ ID - 3370
View
3325 - What are the catalog numbers for the packing materials for the QIAcube?
View
What is the expected DNA yield from 2.5 ml of blood collected in the PAXgene Blood DNA Tube (IVD)?
FAQ ID - 3382
View
3335 - Can I use the DNA directly from enzymatic reaction for Epitect Bisulfite treatment with the Epitect Bisulfite kit (cat. no. 59104)?
View
3336 - Can I use the linearized DNA for Epitect Bisulfite treatment with the Epitect Bisulfite kit?
View
3337 - Which method is used for QIAGEN sequencing service?
View
3342 - How is the unmethylated unconverted EpiTect DNA control to be used?
FAQ - 3342
View
3338 - Does the miRNeasy Serum/Plasma Spike-in Control (cat n° 219610) have 3'-hydroxyl and 5'-phosphate groups?
View
3339 - Does the miRNeasy Serum/Plasma Spike-in Control (cat n° 219610) negatively impact subsequent NGS sequencing?
View
3322 - What is the concentration of the reconstituted miScript Primer Assay (100) and miScript Precursor Assay (100) tubes?
View
3315-What is the concentration of the reconstituted QuantiTect Primer Assay (200)?
View
3318 - What guidelines should I follow in designing primers for use with the EpiTect MSP Kit?
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3317 - Do you have a protocol for isolation of genomic DNA from cell cultures using the QIAamp cador Pathogen Mini Kit?
View
3343 - Why is it difficult to quantify DNA using UV/Vis spectroscopy and fluorescence methods following EpiTect Bisulfite Conversion? How best do you quantify it?
FAQ - 3343
View
3318 - What is the preferred amplicon size for performing PCR on DNA amplified with the EpiTect Whole Bisulfitome Kit?
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3321 - What is the purpose of the C. elegans miR-39 spike-in control in the miRNeasy Serum/Plasma kit?
View
3320 - What is the sensitivity of the Type-it HRM PCR Kit?
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Can I buy the RNeasy Mini columns, RNeasy MinElute columns, RNeasy Midi columns or the RNeasy Maxi columns separately?
FAQ ID - 3388
View
3305 - What is the minimal amount of starting material required for using the SBTexcellerator HLA Kits?
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Do you have data to show lowest dropout rate with Repli-G WTA Single Cells kit?
FAQ ID - 3392
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