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QIAquick® Spin Columns can now be used on any vacuum manifold with luer connectors, for example, the QIAvac 6S or QIAvac 24 with QIAvac Luer Adapters. This protocol is designed for removal of primers <10 bases, enzymes, salts, and unincorporated nucleotides from biotin-, or DIG-labeled DNA fragments and oligonucleotides >17 nucleotides.
Supplementary protocol using the EZ1 Advanced or EZ1 Advanced XL with the EZ1 DNA Investigator Kit
The following protocol is optimized for transient transfection of 293 cells in 96-well plates without pre-plating of cells 24 h prior to transfection. Cell plating and transfection are performed on the same day, making this protocol rapid and convenient.
The following protocol is optimized for transient transfection of CHO cells in 96-well plates without pre-plating of cells 24 h prior to transfection. Cell plating and transfection are performed on the same day, making this protocol rapid and convenient.
The following protocol is optimized for transient transfection of COS-7 cells in 96-well plates without pre-plating of cells 24 h prior to transfection. Cell plating and transfection are performed on the same day, making this protocol rapid and convenient.
The following procedure is for co-transfection of adherent cells using siRNA and plasmid DNA in one well of a 24-well plate. This procedure is provided as a starting point for optimization of siRNA and plasmid DNA co-transfection in mammalian cells using TransMessenger™ Transfection Reagent.
For parallel preparation of genomic, bacterial, or viral DNA or viral RNA from more than 24 samples, we recommend using QIAamp® Spin Columns with 4-6 ml collection tubes. Any tube with an inner diameter of 9-10 mm (outer diameter 11-12 mm) is suitable.
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