For concentration and purification of viral RNA and DNA from serum and plasma
  • Rapid purification of high-quality, ready-to-use viral DNA and RNA
  • No organic extraction or alcohol precipitation
  • Consistent, high yields
  • Removal of contaminants and inhibitors

The QIAamp UltraSens Virus Kit uses a novel technology to concentrate viral nucleic acids in plasma and serum samples, followed by nucleic acid purification using proven QIAamp technology. Starting with sample volumes of up to 1 ml, nucleic acid concentration is achieved by first adding a novel reagent to the sample. The reagent forms complexes with nucleic acids, allowing them to be highly concentrated by low-speed centrifugation.

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QIAamp UltraSens Virus Kit (50)
For 50 viral nucleic acid preps: 50 QIAamp Mini Spin Columns, Proteinase K, carrier RNA, Collection Tubes (2 ml), buffers
53704
$463.00
QIAamp UltraSens Virus Kit (250)
For 250 viral nucleic acid preps: 250 QIAamp Mini Spin Columns, Proteinase K, carrier RNA, Collection Tubes (2 ml), buffers
53706
$1,990.00
The QIAamp UltraSens Virus Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
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QIAamp UltraSens procedure.|High performance of purified nucleic acids in downstream analyses.|High performance in RT-qPCR.|
|RNA purified from a typical virus using the QIAamp UltraSens procedure was used in one-step RT-PCR with the LightCycler. geq/ml: genome equivalents per milliliter; : negative control; M: markers. Data kindly provided by Dr. Thomas Laue, Artus GmbH, Hamburg, Germany.|RNA purified from a typical virus using the QIAamp UltraSens procedure was used in quantitative, real-time RT-PCR with the LightCycler. geq/ml: genome equivalents per milliliter; : negative control; M: markers.|
Performance

The QIAamp UltraSens Virus Kit is suitable for purification of viral nucleic acids from a wide range of viral species. It can also be used for efficient isolation of extracellular genomic DNA from plasma and serum. The purified nucleic acids perform well in sensitive downstream applications (see figures "High performance in one-step PCR" and "High performance in RT-PCR").

The purified DNA and RNA can be used in a wide range of downstream applications, including:

PCR and quantitative real-time PCR
Infectious disease research
Principle

No phenol–chloroform extraction is required. Nucleic acids bind specifically to the QIAamp silica-gel membrane while contaminants pass through. PCR inhibitors, such as divalent cations and proteins, are completely removed in two efficient wash steps, leaving pure nucleic acids to be eluted in either water or a buffer provided with the kit.

QIAamp technology yields viral RNA and DNA from cell-free body fluids that are ready to use in PCR and blotting procedures. QIAamp sample preparation technology is fully licensed.

Procedure

Nucleic acid concentration is achieved by first adding a novel reagent to the sample. The reagent forms complexes with nucleic acids, allowing them to be highly concentrated by low-speed centrifugation. This step allows nucleic acid purification from large sample volumes without the inconvenience of handling large volumes throughout the protocol. Viral nucleic acids are then purified using QIAamp silica-gel-membrane technology (see flowchart "Procedure"). Lysates are loaded onto the QIAamp spin column. Wash buffers are used to remove impurities and pure, ready-to-use DNA is then eluted in water or low-salt buffer.

No ultracentrifugation or specialized laboratory equipment is required, and an internal control can be added at the start of the procedure, allowing full monitoring of the purification process.

Applications

The QIAamp UltraSens Virus Kit uses new technology to concentrate viral nucleic acids in plasma and serum samples, followed by nucleic acid purification using proven QIAamp technology. The procedure provides increased sensitivity in viral-load monitoring and other applications where high viral nucleic acid recovery is essential. Samples of 1 ml plasma or serum can be prepared within 1 hour, with typical yields of >85% recovery of nucleic acids and 60 µl elution volume.

Feature
Specifications
Applications PCR, qPCR, real-time PCR
Elution volume 60 µl
Format MinElute columns
Main sample type Serum, plasma
Processing Manual (centrifugation)
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein Viral DNA, viral RNA
Sample amount 1 ml
Technology Silica technology
Time per run or per prep 1 hour
Yield >85% recovery

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Kit Handbooks
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1 ml 血漿あるいは血清からのウィルスRNA、DNAを高い効率で精製
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For highly efficient purification of viral RNA and DNA from 1 ml plasma and serum samples
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