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DNeasy PowerMax Soil Kit (10)
For the isolation of microbial DNA from large quantities of soil with low microbial load
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NoviPure Soil Protein Kit
For the extraction of protein from all soil types
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LifeGuard Soil Preservation (100 ml)
For the ambient temperature stabilization of microbial RNA in the soil
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DNeasy PowerSoil Pro Kit (50)
For the isolation of microbial genomic DNA from all soil types
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RNeasy PowerSoil DNA Elution Kit (25)
For co-isolation of DNA and RNA from soil using the Rneasy PowerSoil Total RNA Kit
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RNeasy PowerSoil Total RNA Kit (25)
For the isolation of high quality total RNA from all soil types
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MagAttract PowerSoil DNA KF Kit (384)
For the automated, hands-free isolation of DNA from soil
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DNeasy PowerLyzer PowerSoil Kit (50)
For the bead-based isolation of DNA from tough soil microbes
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Knowledge Support Web
Soil pathogens are an important and often overlooked component of plant communities. Though they are harmful to individual plants, they positively contribute to the overall diversity and composition of plant communities. While beneficial symbiont communities in prairie ecosystems have been extensively studied, little is known about how pathogenic soil microbes are affected by global climate change and human land use in these systems. Therefore, in this study, we examine the impact of human disturbance and global change on soil pathogen community structure. To study these soil pathogens, we looked at paired sites of remnant prairies, those undisturbed by human activity, and disturbed prairies across a precipitation and temperature gradient in the Midwestern US. Oomycete and fungal DNA was sequenced from extracted soil and run through two custom pipelines. We then used statistical tests to examine differences in community composition and OTU richness across these environmental and human use gradients. Ultimately, we find that different groups of soil microbes react similarly to human land use disturbance across environmental gradients: environmental gradients are important in driving community responses in remnant, but not disturbed, prairies. These results illuminate how microbial pathogens react to their environmental factors, and has implications for restoration success, remnant stability after restoration, and potential invasion by non-native plants.
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DNeasy PowerSoil Kit (50)
For the isolation of microbial genomic DNA from all soil types
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