Why does the template for EasyXpress Disulfide Insect Kits need to have a signal sequence (such as the mellitin signal sequence as provided by the EasyXpress Linear Template Fab Kit primers)?
FAQ ID -2967

The signal sequence ensures that the expressed protein is quantitatively translocated into the vesicles contained in the lysate. These vesicles represent a distinct compartment derived from the endoplasmic reticulum (ER); they contain the enzymes and chaperones from the ER which are responsible for correct folding and posttranslational modifications such as disulfide bond formation and glycosylation.