How fast is the 6xHis-tagged protein purification process using Ni-NTA Superflow Cartridges?

A maximum of 1 hour is needed for the complete purification process with Ni-NTA Superflow Cartridges when recommended flow rates are applied. This is true for 1 ml and 5 ml cartridges.

Details for a typical 1 ml column run:

  • Equilibration (5 column volumes (cv)): 5 min
  • Load (10 ml Cleared Lysate): 10 min
  • Wash (10 cv): 10 min
  • Elution (10 cv): 10 min
  • optional: Cleaning-in-place (0.5 M NaOH, 0.5 ml/min, 7.5 cv): 15 min  

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