What are the expected DNA yields from different tissues using the QIAamp DNA Mini Kit?
The expected DNA yields from different tissues using the QIAamp DNA Mini Kit are as follows:
|Sample||Nucleic acid yield without RNase A treatment (ug)||DNA yield with RNase A treatment (ug)|
|Blood (200 ul)||4–12||4–12|
|Buffy coat (200 ul)||25–50||25–50|
Cultured cells (5 x 10e6)
|Liver (25 mg)||60–115||10–30|
|Brain (25 mg)||35–60||15–30|
|Lung (25 mg)||8–20||5–10|
|Heart (25 mg)||25–45||5–10|
|Kidney (25 mg)||40–85||15–30|
|Spleen (10 mg)||25–45||5–30|
DNA was purified with QIAamp Kits following standard protocols.