FAQ-147916
Is it possible to test the efficiency of FastSelect rRNA removal by using a Bioanalyzer, etc.?
No, it is not possible to test the efficiency of the FastSelect reaction by running a portion of the eluate from the bead cleanup on a Bioanalyzer®, TapeStation®, Fragment Analyzer®, etc. FastSelect works by inhibiting reverse transcription of rRNA, which doesn’t occur until the first-strand synthesis reaction during library prep.
Related products
QIAseq FastSelect –rRNA Plant Kits
QIAseq FastSelect –rRNA Plant Kits use a novel method to remove highly abundant RNA that is of low scientific value from your RNA-seq libraries. Researchers using RNA-seq for whole transcriptome analysis can use QIAseq FastSelect –rRNA Plant Kits to remove cytoplasmic (5.8S, 18S and 25S), mitochondrial (5S, 18S and 26S) and chloroplast (4.5S, 5S, 16S and 23S) rRNA.
Analyze RNA-seq data with ease using the GeneGlobe-integrated RNA-seq Analysis Portal – an intuitive, web-based data analysis solution created for biologists and included with this QIAseq Kit.
We recommend using QIAseq Stranded RNA Library Kits for robust strand-specific RNA-seq library preparation for both high-quality and highly fragmented RNA.
Design your own custom QIAseq FastSelect pools to remove any RNAs you wish from your RNA-seq library – take a look at our QIAseq FastSelect Custom RNA Removal Kits.
Want to try the QIAseq FastSelect –rRNA Plant Kit for the first time? Request a trial kit to evaluate.
QIAseq FastSelect –rRNA Yeast Kits
QIAseq FastSelect –rRNA Yeast Kits use a novel method to remove highly abundant RNA that is of low scientific value from your RNA-seq libraries. Researchers using RNA-seq for whole transcriptome analysis can use QIAseq FastSelect –rRNA Yeast Kits to remove cytoplasmic (35S [made up of 25S, 18S and 5.8S], 25S, 18S, 5.8S and 5S) and mitochondrial (21S, 15S, ACI60_gr01 [large subunit ribosomal RNA] and ACI60_gr02 [small subunit ribosomal RNA]) rRNA.
Analyze RNA-seq data with ease using the GeneGlobe-integrated RNA-seq Analysis Portal – an intuitive, web-based data analysis solution created for biologists and included with this QIAseq Kit.
We recommend using QIAseq Stranded RNA Library Kits for robust strand-specific RNA-seq library preparation for both high-quality and highly fragmented RNA.
Design your own custom QIAseq FastSelect pools to remove any RNAs you wish from your RNA-seq library – take a look at our QIAseq FastSelect Custom RNA Removal Kits.
Want to try the QIAseq FastSelect –rRNA Yeast Kit for the first time? Request a trial kit to evaluate.