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Buffer AVE (108 x 2 ml)
Cat. No. / ID: 1020953
Buffer AVE for elution of RNA
The QIAamp Viral RNA Mini Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
✓ 24/7 automatic processing of online orders
✓ Knowledgeable and professional Product & Technical Support
✓ Fast and reliable (re)-ordering
Features
- Rapid isolation of high-quality, ready-to-use RNA
- No organic extraction or alcohol precipitation
- Consistent, high yields
- Complete removal of contaminants and inhibitors
Product Details
The QIAamp Viral RNA Mini Kit simplifies purification of viral RNA from cell-free body fluids with fast spin-column or vacuum procedures. Viral RNA binds specifically to the QIAamp silica membrane, and pure viral RNA is eluted in either water or a buffer provided with the kit. Purification can be fully automated on the QIAcube Connect.
Performance
The high-quality viral RNA isolated using the QIAamp Viral RNA Mini Kit performs well in a wide range of downstream applications, including viral genotyping, viral epidemiology, and infectious disease research (see figure " Amplification of RNA from plasma").
See figures
Principle
The QIAamp Viral RNA Mini Kit simplifies isolation of viral RNA from cell-free body fluids with fast spin-column or vacuum procedures. No phenol–chloroform extraction is required. Viral RNA binds specifically to the QIAamp silica membrane while contaminants pass through. PCR inhibitors, such as divalent cations and proteins, are completely removed in two efficient wash steps, leaving pure viral RNA to be eluted in either water or a buffer provided with the kit.
QIAamp RNA technology yields viral RNA from cell-free body fluids ready to use in RT-PCR and blotting procedures. QIAamp sample preparation technology is fully licensed.
QIAamp RNA technology yields viral RNA from cell-free body fluids ready to use in RT-PCR and blotting procedures. QIAamp sample preparation technology is fully licensed.
Procedure
Optimized buffers and enzymes lyse samples, stabilize nucleic acids, and enhance selective RNA adsorption to the QIAamp membrane. To guarantee RNA integrity, samples are lysed under highly denaturing conditions to inactivate RNases. Alcohol is added and lysates loaded onto the QIAamp spin column. Wash buffers are used to remove impurities and pure, ready-to-use RNA is then eluted in water or low-salt buffer.
The QIAamp Viral RNA Mini Kit is automatable on the QIAcube. The QIAamp Viral RNA Mini Accessory Set provides the additional buffers and reagents needed for automated, low-throughput sample prep using both QIAcube and QIAamp Viral RNA Mini Kit.
Vacuum processing
For greater speed and convenience in RNA purification, samples can be processed by vacuum instead of centrifugation. QIAamp Mini spin columns (see figure "QIAamp Viral RNA Mini spin column") are accommodated on the QIAvac 24 manifold using VacValves and VacConnectors, provided in the QIAamp Vac Accessory Set. VacValves should be used if sample flow rates differ significantly, in order to ensure consistent vacuum. Disposable VacConnectors are used to avoid any cross-contamination.
Applications
The QIAamp Viral RNA Mini Kit simplifies purification of viral RNA from cell-free body fluids, offering fast spin-column or vacuum procedures, or automation on QIAcube. Sample types include:
- Plasma and serum
- CSF
- Urine
- Other cell-free body fluids
- Cell-culture supernatants
Supporting data and figures
Amplification of RNA from plasma.
RT-PCR products of a 1026 nt RNA fragment purified from plasma. Serial tenfold dilutions (as indicated) were added to plasma and purified using the QIAamp Viral RNA Mini Kit. M: markers; C: negative control.
Specifications
| Features | Specifications |
| Applications | PCR, qPCR, real-time PCR |
| Time per run or per prep | 20–40 minutes |
| Technology | Silica technology |
| Elution volume | 50 µl |
| Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein | Viral RNA |
| Processing | Manual (centrifugation or vacuum) |
| Sample amount | 140 µl |
| Format | Spin columns |
| Main sample type | Liquid media |
| Yield | >90% recovery |
Resources
Safety Data Sheets (1)
Publications
Mutation of a diacidic motif in SIV-PBj Nef impairs T-cell activation and enteropathic disease.
Retrovirology; 2011; 8 :14 2011 Mar 2 PMID:21366921
Detection and differentiation of tick-borne encephalitis virus subtypes by a reverse transcription quantitative real-time PCR and pyrosequencing.
J Virol Methods; 2010; 171 (1):34-9 2010 Oct 7 PMID:20933016
Efficacy and safety of an antiviral Iota-Carrageenan nasal spray: a randomized, double-blind, placebo-controlled exploratory study in volunteers with early symptoms of the common cold.
Respir Res; 2010; 11 (1):108 2010 Aug 10 PMID:20696083
Oseltamivir-resistant influenza A 2009 H1N1 virus in immunocompromised patients.
Influenza Other Respir Viruses; 2010; 4 (4):199-204 2010 Jul PMID:20836794
Conserved charged amino acids within Sendai virus C protein play multiple roles in the evasion of innate immune responses.
PLoS One; 2010; 5 (5):e10719 2010 May 19 PMID:20502666
FAQ
Is QIAGEN carrier RNA sold separately?
Do you have a protocol for the isolation of viral RNA from stool?
What is the difference between QIAGEN Protease and QIAGEN Proteinase K provided in various QIAamp Kits?
Why is the QIAamp Viral RNA Mini Kit (50) currently unavailable?
What can be used as an alternative to the A260 measurement for quantification of small amounts of RNA and DNA?
What is the average amount of DNA and RNA present in 1 ml normal serum?
What has to be done to an RNA sample before loading it onto an Agilent Bioanalyzer?
How do I safely inactivate biohazardous flow-through material?