For the rapid and RNA-safe degradation of genomic DNA

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The dsDNase is intended for molecular biology applications. This product is neither intended for the diagnosis, prevention, or treatment of a disease, nor has it been validated for such use either alone or in combination with other products.
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  • Active in a broad temperature range (10–80°C) and broad pH range (optimum at pH 6.0–9.0)
  • Highly active at elevated salt concentrations and other typical buffer additives
  • Requires bivalent cations (Mg2+ and Ca2+) for maximum activity
  • Degrades dsDNA to fragments below 10 nt
  • Activity towards dsDNA is at least 1000 times higher than towards ssDNA

Product Details

dsDNase is a 42.8 kDa recombinant endonuclease derived from marine amphipods expressed in Pichia pastoris. The enzyme displays high specific activity towards double-stranded DNA, leaving single-stranded DNA or RNA undamaged in standard conditions. dsDNase is highly active in a broad spectrum of temperatures, buffer conditions and pH.

These features make DNaseMe extremely useful for rapid and RNA-safe degradation of genomic DNA, where the absence of ribonucleases is critical to maintaining the integrity of RNA.

The enzyme hydrolyzes phosphodiester linkages yielding oligonucleotides with a 5′-phosphate and 3′-hydroxyl groups.

It is supplied with 20 mM Tris-HCl, pH 8.0; 50 mM KCl; 5 mM MgCl2 ; 50% (v/v) glycerol.

One unit (U) is defined as the amount of enzyme that causes an increase in absorbance at 260 nm of 1.0 in 30 minutes at 37°C and pH 8.0 with herring sperm DNA as a substrate.


Assay Specification
Protein Purity >95%
RNase activity None detected
Protease activity None detected


The specific activity is similar to bovine DNase I; however, dsDNase is characterized by higher stability in demanding reaction and storage conditions (e.g., high salt and detergent-containing buffers and elevated temperature).


Quality Control 

Protein concentration was determined by SDS-PAGE electrophoresis using Coomassie Blue detection assay.
The presence of RNase activity was determined by gel electrophoresis following incubation of 1 µg of total eukaryotic RNA with 10 U of enzyme in a 20 mL volume for 1 hour at 37°C.
The presence of protease activity was determined by SDS-PAGE with Coomassie Blue detection following incubation of 1 mg of BSA with 100 U of enzyme for 20 hours at 37°C.
A 1.575 mL reaction volume containing herring sperm DNA as a substrate is incubated for 30 minutes at pH 8 at 37°C with an enzyme that degrades DNA to acid-soluble oligonucleotides. One unit of the enzyme causes an increase in absorbance at A260 of 1.0.


This is used for applications such as:

  • Extraction and purification of RNA
  • Removal of contaminating genomic DNA from RNA samples
  • Degradation of DNA template in transcription reactions
  • Reduction of viscosity in biological samples
  • Removal of residual DNA during primary stem cell isolation, biopharma and bioprocessing procedures


Safety Data Sheets (1)