As little as 0.01 pg E. coli and 0.05 pg Candida were successfully detected and NTC showed no background.
The DNA Polymerase is kept in an inactive state by the Antibody and Guard Molecule until the initial heat-activation step.
The UCP Multiplex PCR Kit provides a convenient format for highly-sensitive and specific multiplex hot-start PCR using any DNA or cDNA template. The kit comes with the UCP Multiplex PCR Master Mix, UCP Template Tracer, UCP Master Mix Tracer and UCP Water.
[A] NH4+ ions prevent nonspecific primers from annealing to the template. Synthetic Factor MP, an innovative PCR additive, increases the local concentration of primers at the template. [B] Together with K+ and other cations, synthetic Factor MP stabilizes specifically bound primers, allowing efficient primer extension by DNA Polymerase.
PCR procedure using tracer dyes.
Easy visualization with orange and blue tracking dyes.