Consistent amounts of BVDV particles and Salmonella cells were simultaneously spiked into a variety of animal specimens. Samples were processed in parallel on the QIAcube HT with the cador Pathogen 96 QIAcube HT Kit or manually with the QIAamp cador Pathogen Mini Kit. Co-isolated viral RNA and bacterial DNA were identified using an in-house assay. Mean CT values are shown for [A] BVDV and [B]Salmonella. Small error bars (±1 SD of 3 extraction replicates) indicate high precision for both purification procedures.
Blood samples of various species were spiked with BVDV particles and were frozen at –20°C. Of each sample, 4 replicates were processed on QIAcube HT with the cador Pathogen 96 QIAcube HT Kit in 3 subsequent runs on different days. [A] Viral RNA was identified using an in-house assay, which included [B] an internal amplification control. Error bars represent ±1 SD of 3 replicates. The BVDV CT values for each set of quadruplicates showed a mean relative standard deviation of 0.67 ± 0.43%, and the inter-assay CV for each sample from 3 runs ranged from 0.14 to 0.89% (mean: 0.45%), indicating high repeatability of viral RNA purification on QIAcube HT.
Consistent amounts of BVDV particles and Salmonella cells were simultaneously spiked into a variety of animal specimens. Samples were processed in parallel on the QIAcube HT with the cador Pathogen 96 QIAcube HT Kit or a kit from supplier A. Co-isolated viral RNA and bacterial DNA were identified using in-house assays. Mean CT values are shown for [A] BVDV and [B]Salmonella. Error bars represent ±1 SD of 3 extraction replicates. Samples purified on the QIAcube HT resulted in lower CT values for both bacterial DNA and viral RNA from all starting materials tested.
