Fast, sensitive analysis of RNA
RNA quality is a critical factor for reliable results in gene expression studies and miRNA research, in which sensitive technologies such as real-time PCR or microarray analysis are used. Monitoring the integrity of the initial total RNA sample as well as products generated throughout the entire procedure is crucial, since their integrity strongly influences the predictive power of the detection method. Fully automated QIAxcel instruments enable fast and sensitive analysis of the integrity and quantity of total RNA and fragmented or intact cRNA (see figure Streamlined RNA analysis using the QIAxcel system
). Automated sample loading and analysis reduce manual handling of samples, minimizing the risk of RNA degradation and contamination. The degradation state of samples can be easily analyzed on QIAxcel instruments (see figures Analysis of degraded RNA
and Analysis of plant RNA
Objective quality values with the new RNA Integrity Score
The new QIAGEN RNA Integrity Score (RIS) provides an objective quality measurement for eukaryotic RNA samples and allows easy interpretation of sample integrity. The RIS is a value from 1 to 10 where a value of 10 indicates completely intact RNA (see figure RIS indicates RNA integrity
). The highly reproducible RIS eliminates the need for human interpretation and enables the implementation of rigorous quality control at appropriate breakpoints across workflows. This allows researchers to eliminate samples that do not meet the quality criteria required for downstream processes, reducing the risk of needing to repeat experiments and saving time and costs.
RNA analysis on QIAxcel instruments is highly reproducible and gives results comparable to the Agilent Bioanalyzer.