Unique rotary design for outstanding performance
The Rotor-Gene Q MDx uses a sophisticated heating and cooling design to achieve optimal reaction conditions. The unique rotary format ensures optimal thermal and optical uniformity between samples, which is critical for precise and reliable analysis (see figure " Cross-section of the Rotor-Gene Q MDx"). Each tube spins in a chamber of moving air, keeping all samples at precisely the same temperature during rapid thermal cycling. Detection is similarly uniform. When each tube aligns with the detection optics, the sample is illuminated and the fluorescent signal is rapidly collected from a single, short optical pathway. This thermal and optical uniformity results in sensitive, precise, and fast real-time PCR analysis.
Robust optics for multiplex applications
With a choice of up to 6 excitation sources and 6 detection filters (the red and HRM channels are not intended for use with FDA cleared or approved nucleic acid tests), combined with a short, fixed optical path, the Rotor-Gene Q MDx can be used for multiplex reactions, ensuring minimum fluorescence variability between samples and eliminating the need for calibration or compensation.
Samples are excited from the bottom of the chamber by a light-emitting diode (see figure " Cross-section of the Rotor-Gene Q MDx"). Energy is transmitted through the thin walls at the base of the tube. Emitted fluorescence passes through emission filters on the side of the chamber and is then collected by a photomultiplier. The fixed optical path ensures consistent excitation for every sample, which means that there is no need to use a passive internal reference dye such as ROX.
Channels for optical detection
|Channel ||Excitation (nm)||Detection (nm)|
|Green ||470 ± 10 ||510 ± 5|
|Yellow ||530 ± 5 ||557 ± 5|
|Orange ||585 ± 5 || 610 ± 5|
|Red* ||625 ± 5 ||660 ± 10|
|Crimson ||680 ± 5 ||712 high pass|
|HRM* ||460 ± 20 ||510 ± 5|
Minimum maintenance; maximum convenience
The Rotor-Gene Q MDx is engineered to reduce the need for maintenance, and to maximize ease of use. This saves time and costs, and keeps the instrument operational for continuous testing.
Convenient features of the Rotor-Gene Q MDx include:
- Lifetime guarantee on highly stable LEDs, no expensive and time consuming lamp replacement, and no gradual performance loss
- Cost-effective and easy temperature-accuracy verification
- Optical calibration not required at installation, or when the instrument is moved
- "Plug and play" connectivity between the computer and the instrument
- No sample block to clean
- No condensation or bubbles in the reaction due to rotation
- Small, light, and robust instrument — simply place it where you want it