The Investigator 26plex QS Kit uses QIAGEN's fast-cycling PCR technology for the rapid, simultaneous amplification of the Chinese, CODIS and European standard markers, as well as Amelogenin. Carefully designed primers minimize the overlap of alleles of different makers, thereby minimizing the likelihood of misinterpretation of data.
The innovative Quality Sensors provide additional information on the sample quality and PCR success beyond the profile of the sample. With two Quality Sensors being amplified as a positive control, it is possible to determine:
If the PCR amplification worked
If there was any DNA in the sample
If the sample was degraded
If inhibitors in the sample prevented successful PCR
This additional information will greatly enhance how you analyze your data and aid you in making crucial decisions with respect to further sample processing. Ultimately, the Quality Sensors will help you reduce unnecessary re-runs, saving you time and money.
The optimized design of the two gender markers, Amelogenin and DYS391, enables the Investigator 26plex QS Kit to have both at a small fragment size, making the gender typing extremely robust. Even for populations with Amelogenin Null mutations, this means no more failures with the essential gender typing, even with degraded or inhibited samples.