The MiSeq FGx System is integral to a validated workflow designed specifically for forensic laboratories. The system is fully supported with performance qualifications and regular maintenance that align with the requirements of a forensic laboratory. Building on the speed and accuracy of Illumina sequencing-by-synthesis (SBS) chemistry, the MiSeq FGx System brings the unprecedented focus of forensic genomics to NGS, granting criminal justice a powerful ally.
- Superior analysis of complex DNA mixtures and degraded DNA
- Integrated sequencing workflow consolidates library prep, sequencing, and analysis
- Advanced capability with adjustable read lengths, dual run modes and two flow cells
The MiSeq FGx System is the first and only instrument to interrogate up to 96 combined SNP and STR libraries in a single run. The MiSeq FGx System preserves precious sample while demonstrating robust performance, developing more thorough, detailed DNA profiles from a wide range of sample types, biological molecules, and across the quality spectrum of high-quality genomic DNA (gDNA) to degraded, mixed and limited samples.
Two run modes promote a range of forensic genomics applications while retaining the flexibility to work in a research environment. Easily switch between two sequencing kits to tune output for the current application or sample batch size. Built on industry-leading Illumina SBS chemistry, the MiSeq FGx System meets strict performance requirements for comprehensive and conclusive results. Virtually eliminate context-specific errors, even within repetitive sequence regions or homopolymers and answer a wider range of questions in one targeted assay with multiplexing capability. An intuitive touchscreen interface simplifies instrument operation and the system monitors quality and performance metrics during a run. After sequencing, analysis starts automatically and completes in under one hour.
||1–384 samples per run, depending on assay
|Maximum read length
||2 x 300 bp, depending on assay
|Output (2 x 150 bp run)
||≥ 5 Gb
|Reads passing filters
|Q30 score (at read length of 2 x 150 bp)
|Total overall accuracy
|Total overall reproducibility
|Biomolecules that can be sequenced
||gDNA, RNA, mtDNA
||Forensic (FGx) and Research Use Only (RUO)
||100–240 V AC at 50/60Hz, 10A, 400 W
|RFID radio frequency
||68.6 cm × 56.5 cm × 52.3 cm (27 in × 22.2 in × 20.6 in
||54.5 kg (120 lbs)