text.skipToContent text.skipToNavigation

QIAseq Library Quant System

For qPCR-enabled quantification of NGS libraries
  • Ready-to-use, predispensed, sequentially diluted DNA standard (array format)
  • Automatic calculation of library concentration
  • Simple protocol, high sensitivity, and wide dynamic range
  • Compatibility with Illumina and Ion Torrent/Proton NGS platforms
  • Compatibility with most qPCR instruments

The QIAseq Library Quant System provides a simple, out-of-the-box solution to quantify NGS libraries, enabling consistent results with every NGS run. The QIAseq Library Quant System uses real-time PCR to specifically quantify DNA molecules with sequencing adaptors at both ends, ensuring optimal cluster density or template-to-bead ratio.

Please note: GeneRead Library Quant Array (product number 180611) and GeneRead Library Quant Kit (product number 180612) will be phased out by the end of 2016. Please use QIAseq Library Quant Assay Kit (product number 333314) instead.


Need a quote for your research project or would you like to discuss your project with our specialist team? Contact Us

Buy Products

Cat No./ID: 333304
GeneRead Library Quant Array
Go to GeneGlobe
PCR arrays with accessory components for sample library quantification prior to next-generation sequencing
Cat No./ID: 333314
QIAseq Library Quant Assay Kit
$440.00
Go to GeneGlobe
1 tube of Primer Mix (500 µl), 1 bottle of Dilution Buffer (30 ml), 1 tube DNA Standard (100 µl), and 5 tubes GeneRead qPCR SYBR Green (1.35 ml) for sample library quantification prior to NGS
The QIAseq Library Quant System is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

Product Details

9
The QIAseq Library Quant System enables quantification of libraries with concentrations below the detection limit of conventional methods
The QIAseq Library Quant Array's high sensitivity and broad dynamic range enable the quantification of both the NGS-L1 and NGS-L2 libraries [A]. By contrast, Agilent's High Sensitivity DNA Kit (for use with the Agilent 2100 BioAnalyzer) quantified only the NGS-L1 library; with this kit, the NGS-L2 concentration was too low for quantification [B].
9
Principle of the QIAseq Library Quant System
The serial dilutions of the DNA standard (5 sequential 10-fold dilutions) generate a standard curve. The sample library should fall within the standard curve.
9
QIAseq Library Quant Array workflow
Each sample DNA library is combined with QIAseq qPCR SYBR® Green Mastermix, aliquoted in technical triplicate across the QIAseq Library Quant Array, and analyzed with qPCR. Raw CT values are copied into the data analysis Excel template, and the software automatically calculates the library concentration or dilution factor.
Performance

The sensitivity and dynamic range of the QIAseq Library Quant System were compared against Agilent's High Sensitivity DNA Kit, using two NGS libraries of differing concentrations. The first library was quantifiable by both systems, but the low concentration of the second library was below the detection limit of Agilent's 2100 BioAnalyzer. By contrast, the QIAseq Library Quant Array was able to quantify both libraries (see figure QIAseq Library Quant System enables quantification of libraries with concentrations below the detection limit of conventional methods).

Principle
Accurate quantification of library molecules is essential for the efficient use of NGS platforms; however, current spectrophotometric methods only measure total nucleic acid concentrations. The QIAseq Library Quant System, by contrast, uses real-time PCR to quantify only DNA molecules with adaptors at both ends, which are the only amplifiable molecules during emulsion PCR (Ion Torrent platform) or bridge PCR (Illumina platform), and therefore provides highly accurate quantification of amplifiable library molecules. The high sensitivity of real-time PCR allows quantification of libraries at very low concentrations, even below the detection threshold of conventional spectrophotometric methods.

The QIAseq Library Quant System is available in both a tube and an array format, and is optimized with QIAseq qPCR SYBR® Green Mastermixes to provide superior sensitivity and a wide dynamic range. Both formats provide a DNA standard specific to the Illumina or Ion Torrent platform. The array format provides this standard in five predispensed, sequential 10-fold dilutions mixed with a PCR primer assay in triplicate, ensuring that your sample library will fall within the detection range of the array (see figure Principle of QIAseq Library Quant System).
Procedure

The array format workflow (see figure QIAseq Library Quant Array workflow) begins with two tenfold dilutions of the sample library, to ensure that its concentration falls within the range of the serially diluted standards. The samples are mixed with QIAseq qPCR SYBR Green Mastermix and aliquoted in technical triplicate across the array plate. PCR is performed, and raw CT values are exported to the provided data analysis Excel file for automatic calculation of library concentration (Illumina platform) or template dilution factor (Ion Torrent/Proton platform).

The tube format workflow is similar to the array format workflow, with a few small differences. The procedure begins with preparation of five sequential tenfold dilutions of DNA Standard and two tenfold dilutions of the sample library. This ensures that the concentration of the library will fall within the range of the standard dilutions. Next, the diluted DNA standards and sample libraries are mixed with the provided PCR assay and the appropriate QIAseq qPCR SYBR Green Mastermix. PCR is performed, and CT values are exported to the provided data analysis Excel file for automatic calculation of library concentration (Illumina platform) or template dilution factor (Ion Torrent/Proton platform).

Applications
The QIAseq Library Quant Array and QIAseq Library Quant Kit provide rapid, accurate measurement of sample library concentration prior to sequencing with the Illumina or Ion Torrent/Proton NGS platforms.

Product Resources

You are not authorized to download the resource

Brochures & Guides (2)
Accelerate your NGS performance through Sample to Insight solutions
Show details
Accelerate your NGS performance through Sample to Insight solutions
View
Analysis Software (8)
New version – Excel-based data analysis template for use with GeneRead Library Quant Kit (assay format) or GeneRead Library Quant Array for 384-well plates for Illumina libraries
Show details
New version – Excel-based data analysis template for use with GeneRead Library Quant Kit (assay format) or GeneRead Library Quant Array for 384-well plates for Ion Torrent libraries
Show details
New version – Excel-based data analysis template for use with GeneRead Library Quant Kit (assay format) or GeneRead Library Quant Array for 96-well plates for Illumina libraries
Show details
New version – Excel-based data analysis template for use with GeneRead Library Quant Kit (assay format) or GeneRead Library Quant Array for 96-well plates for Ion Torrent libraries
Show details
New version – Excel-based data analysis template for use with GeneRead Library Quant Kit (assay format) or GeneRead Library Quant Array for Rotor-Disc 100 rings for Illumina libraries
Show details
New version – Excel-based data analysis template for use with GeneRead Library Quant Kit (assay format) or GeneRead Library Quant Array for Rotor-Disc 100 rings for Ion Torrent libraries
Show details
New version – Excel-based data analysis template for use with GeneRead Library Quant Kit (assay format) for 72-well Rotor rings for Illumina libraries
Show details
New version – Excel-based data analysis template for use with GeneRead Library Quant Kit (assay format) for 72-well Rotor rings for Ion Torrent libraries
Show details
fragment fix placeholder

Customers who bought these products also bought

  • Cat No./ID: 333602
    /us/products//discovery-and-translational-research/next-generation-sequencing/rna-sequencing/rna-fusions/qiaseq-targeted-rnascan-panel/

    QIAseq Targeted RNAscan Panel (12)

    Kit containing ALL reagents (except indexes) for targeted Fusion sequencing; fixed panel for 12 samples; less than 100 fusions
  • Cat No./ID: 333945
    /us/products//discovery-and-translational-research/next-generation-sequencing/multianalyte-sequencing-multimodal/qiaseq-multimodal-panels/

    QIAseq Multimodal HC Panel (96)

    Kit containing ALL reagents (except indices) sufficient to process 96 samples for multimodal (DNA and RNA) sequencing; fixed high content (HC) panel for a total of 192 reactions

  • Cat No./ID: 333512
    /us/products//discovery-and-translational-research/next-generation-sequencing/dna-sequencing/whole-genome-sequencing/qiaseq-targeted-dna-panels/

    QIAseq Targeted DNA HC Panel (12)

    Kit containing ALL reagents (except indexes) for targeted DNA sequencing; fixed panel for 12 samples; more than 100 genes
  • Cat No./ID: 333896
    /us/products//discovery-and-translational-research/next-generation-sequencing/rna-sequencing/qiaseq-sars-cov-2-primer-panel/

    QIAseq SARS-CoV-2  Primer Panel (96)

    Reagents and primers for reverse transcription, whole genome amplification and downstream Illumina sequencing; for 96 reactions

    $960.00
  • Cat No./ID: 333074
    /us/products//discovery-and-translational-research/next-generation-sequencing/rna-sequencing/ultraplex/qiaseq-upx-3-transcriptome-kits/

    QIAseq UPX 3' Trans. 12-Index (48)

    For indexing up to 12 samples per sequencing lane on Illumina NGS instruments; multiple 96- or 384-well plates can be combined as one sample allowing for 12 x 96-well plates (1152 cells/wells) or 12 x 384-well plates (4608 cells/wells) per lane
    $1,033.00
  • Cat No./ID: 333414
    /us/products//discovery-and-translational-research/next-generation-sequencing/sample-quality-control/qiaseq-dna-quantimize-kits/

    QIAseq DNA QuantiMIZE Assay Kit

    Assay 100 and Assay 200 for 400 x 25 µl reactions (400 µl) each; QuantiMIZE Control gDNA (50 µl); RNase- and DNase-free water (1 ml x 4); GeneRead qPCR SYBR Green Mastermix (1.35 ml x 4)

  • Cat No./ID: 333502
    /us/products//discovery-and-translational-research/next-generation-sequencing/dna-sequencing/whole-genome-sequencing/qiaseq-targeted-dna-panels/

    QIAseq Targeted DNA Panel (12)

    Kit containing ALL reagents (except indexes) for targeted DNA sequencing; fixed panel for 12 samples; less than 100 genes
  • Cat No./ID: 333090
    /us/products//discovery-and-translational-research/next-generation-sequencing/rna-sequencing/ultraplex/qiaseq-upx-3-transcriptome-kits/

    QIAseq UPX 3' Transcriptome Kit (384)

    For 3' gene expression analysis from cells or mRNA of 384 samples simultaneously; each kit contains cell lysis solution, buffers, oligos and enzymes lyophilized on a 384-well plate for processing 384 samples in parallel (this format is best suited for large samples numbers of at least 384 samples)
    $8,995.00
  • Cat No./ID: 333714
    /us/products//discovery-and-translational-research/next-generation-sequencing/dna-sequencing/qiaseq-indexes/

    QIAseq 12-Index I (48)

    Box containing combinatorial dual-indexed adapters, for indexing up to 12 samples for QIAseq Targeted Panel sequencing on Illumina platforms; enough to process a total of 48 samples

    $1,211.00
  • Cat No./ID: 333895
    /us/products//discovery-and-translational-research/next-generation-sequencing/rna-sequencing/qiaseq-sars-cov-2-primer-panel/

    QIAseq SARS-CoV-2 Primer Panel (24)

    Reagents and primers for reverse transcription, whole genome amplification and downstream Illumina sequencing; for 24 reactions

    $260.00
  • Cat No./ID: 333089
    /us/products//discovery-and-translational-research/next-generation-sequencing/rna-sequencing/ultraplex/qiaseq-upx-3-transcriptome-kits/

    QIAseq UPX 3' Transcriptome Kit (96-M)

    For 3' gene expression analysis from cells or mRNA of up to 384 samples; each kit contains cell lysis solution, buffers, oligos and enzymes in a 96-well plate. The increased volumes allow for automation or manual pipetting or where custom protocols are necessary.
    $8,995.00
  • Cat No./ID: 180473
    /us/products//human-id-and-forensics/nextgeneration-sequencing/generead-library-prep-kits/

    QIAseq FX DNA Library Kit (24)

    Buffers and reagents for DNA fragmentation (including end repair and A-addition), ligation and library amplification; for use with Illumina instruments; includes a plate containing 24 combinatorial dual index adapters with different bar codes (pierceable foil seal, allowing usage of defined parts of plate)

    $892.00
  • Cat No./ID: 180434
    /us/products//human-id-and-forensics/nextgeneration-sequencing/generead-library-prep-kits/

    GeneRead DNA Library I Core Kit (48)

    For 48 reactions: Buffers and reagents for end-repair, A-Addition, and ligation, for use with Illumina instruments
    $1,113.00
  • Cat No./ID: 180455
    /us/products//human-id-and-forensics/nextgeneration-sequencing/generead-library-prep-kits/

    GeneRead DNA I Amp Kit (100)

    For 100 reactions: Ready-to-use library amplification master mix and primer mix, for use with Illumina instruments
    $374.00
  • Cat No./ID: 180477
    /us/products//discovery-and-translational-research/next-generation-sequencing/metagenomics/qiaseq-fx-dna-library-kit/

    QIAseq FX DNA Library UDI Kit (24)

    Buffers and reagents for DNA fragmentation (including end repair and A-addition), ligation and library amplification; for use with Illumina instruments; includes a plate containing 24 Unique Dual Index Y-adapters.

    $932.00
  • Cat No./ID: 333832
    /us/products//discovery-and-translational-research/next-generation-sequencing/qiaseq-16s-its-smart-control/

    QIAseq 16S/ITS Smart Control (10)

    Synthetic DNA template control sample; sufficient reagents for 10 control samples
    $159.00
  • Cat No./ID: 335927
    /us/products//discovery-and-translational-research/next-generation-sequencing/rna-sequencing/ribosomal-rna-and-globin-mrna-removal/qiaseq-fastselect-5s-16s-23s-kits/

    QIAseq FastSelect –5S/16S/23S Kit (96)

    Includes 1 tube of QIAseq FastSelect –5S/16S/23S rRNA removal reagent, reaction buffer, QIAseq Beads with QIAseq bead binding buffer and nuclease-free water sufficient for 96 samples

    $4,634.00
  • Cat No./ID: 333825
    /us/products//discovery-and-translational-research/next-generation-sequencing/qiaseq-16s-its-index-kits/

    QIAseq 16S/ITS 96-Index I (384)

    Adapters and sample indexes (Set A) for use in conjunction with QIAseq 16S/ITS Panels to generate Illumina-compatible libraries; sufficient adapters for indexing 384 samples (4 x 96 samples)

    $1,297.00
  • Cat No./ID: 333088
    /us/products//discovery-and-translational-research/next-generation-sequencing/rna-sequencing/ultraplex/qiaseq-upx-3-transcriptome-kits/

    QIAseq UPX 3' Transcriptome Kit (96)

    For 3' gene expression analysis from cells or mRNA of 8–96 samples at one time; each kit contains cell lysis solution, buffers, oligos and enzymes lyophilized in a 96-well plate which can be easily separated for processing 8–96 samples in parallel (this format is ideal for both small and large sample sizes)
    $2,960.00