Developed in partnership with leading human identity labs around the world, QIAseq Investigator panels are designed to pick up where conventional STR/capillary testing cannot resolve a case. There are separate, dedicated panels for common human identify questions like biogeographical ancestry prediction, missing persons identification, or paternity testing. Focus your sequencing and data analysis efforts only on the markers of interest in your own case and increase your chances of success with QIAseq.
QIAseq Investigator Missing Persons SNP panel CDHS-15861Z-2897
Highly discriminating identity SNP panel enabling kinship confirmation for challenging missing persons samples using immediate and extended family references.
QIAseq Investigator ID SNP panel CDHS-14055Z-277
Identity SNP panel comprising specially selected kinship SNPs designed for use in kinship and other identification applications.
QIAseq Investigator Global Ancestry SNP panel CDHS-12534Z-204
For accurate and reliable biogeographical ancestry inference using SNPforID AIMs SNPs.
QIAseq Investigator Middle East Ancestry SNP panel CDHS-12533Z-169
Supplementary SNP panel for increased accuracy in biogeographical ancestry inference from putative middle east samples.
QIAseq Investigator Microhaplotype panel CDHS-15034Z-170
Microhaplotype identity panel for high levels of discrimination.
QIAseq Human Mitochondria panel DHS-105Z
Whole mitochondrial genome sequencing in human identity applications.
QIAseq Investigator Human Mitochondria Control Region panel CDHS-13743Z-27
Control Region sequencing for the mitochondrial genome
Do you need a specific customized NGS panel for your human identity investigations not covered by the Investigator panels? Our easy-to-use custom builder lets you design a sequencing panel targeting your regions of interest in just a few clicks.
PCR duplicates are a major issue in targeted DNA sequencing, since, through PCR amplification, they turn unique DNA molecules into identical DNA molecules that cannot be distinguished from each other. In addition, errors from PCR amplification and sequencing process may also be present in final reads that lead to false positive variants in sequencing results. This, in turn, results in the inability to confidently call DNA variants present at low frequencies in the starting DNA material. To overcome the issue of PCR duplicates and amplification artifacts, the QIAseq Targeted DNA Panels use digital sequencing by incorporating molecular barcodes into the starting DNA material before any amplification takes place, thereby preserving the uniqueness of the starting DNA molecules and overcoming the issues of PCR duplicates, false positives and library bias.
The entire workflow of the QIAseq targeted DNA panels to go from extracted DNA to sequencing-ready libraries can be completed in 9 hours (see figure Workflow). Extracted DNA is fragmented, genomic targets are molecularly barcoded and enriched, and libraries are constructed. Sequencing files can be fed into the QIAseq pipeline, a cloud-based data analysis pipeline, which will filter, map and align reads, as well as count unique molecular barcodes associated with targeted genomic regions, and call variants with a barcode-aware algorithm. This data can then be fed into IVA or QCI for interpretation.
The QIAseq targeted DNA panels can be used to call a variety of DNA variants from a wide range of sample types for numerous applications.
Isolated DNA, as low as 20 ng, is enzymatically fragmented to generate small pieces of dsDNA. This is followed by the library construction step, in which IL-N7 adapters, molecular barcodes, and sample indexes are incorporated into DNA fragments generated in the previous step. Library fragments now serve as templates for target enrichment using single primer extension. In this step, targets are enriched using a single gene-specific primer and a universal forward primer. The final step is library amplification and sample indexing (for dual indexing) using the IL-S5 sample index primer and a universal primer.