Investigator Quantiplex Kits

Investigator Quantiplex Kits are ready-to-use quantitative real-time PCR assays for human identity testing and forensic applications. Developed for superior sensitivity and reaction speed, they are designed to establish whether a sample contains sufficient and intact DNA to enable DNA fingerprinting analysis (such as STR, SNP or DIP analysis) and whether there are inhibitors present. Kits are available for quantification of human DNA, or quantification of total human and male DNA. The Investigator Quantiplex Pro technology further enables quality assessment to provide reliable information on the degradation status of your sample. The new IC (Internal Control) in Investigator Quantiplex Pro Kits is more sensitive toward inhibitors and correlates to the STR tolerance while the quantification result remains reliable.

Investigator Quantiplex Kits meet ISO 18385 requirements.

Want to try the Investigator Quantiplex Pro Kit for the first time? Request a quote for a trial kit.

Want to try the Investigator Quantiplex Pro RGQ Kit for the first time? Request a quote for a trial kit.

Investigator Quantiplex Pro Kits provide robust and precise quantification results with high sensitivity within a high dynamic range. In addition to the general sensitivity, the kits provide exceptionally high sensitivity for male DNA, even in a very high background of female DNA (up to 400,000:1).

The straightforward quality assessment system included in the kits comprises a newly developed DNA Degradation Control (DC) and a newly designed Internal Control (IC). The new DNA Degradation Control provides superior detection of DNA degradation (see figures “ Accurate detection of DNA degradation” and “ Accurate detection of male DNA degradation in a sample mixture”). Furthermore, the Investigator Quantiplex Pro RGQ assay is the only commercially available assay that can distinguish between human and male DNA degradation (see figure “ Highly accurate and sensitive detection results for male and total human DNA”). The newly designed Internal Control reliably detects potential PCR inhibitors. The IC is more sensitive to inhibitors and correlates to STR tolerance while the quantification results remain reliable (see figure “ Identification of PCR inhibition”). Furthermore the IC of the Investigator Quantiplex Pro Kits is analogous to the Quality Sensor of Investigator STR QS PCR Assays (e.g., Investigator 24plex QS Kits, Investigator 26plex QS Kits, Investigator ESSplex SE QS Kit and Investigator Argus X-12 QS Kit).

Human identification is commonly based on the analysis of short tandem repeats (STRs), single nucleotide polymorphisms (SNPs) or deletion insertion polymorphisms (DIPs), depending on the demands of an examination or on the sample quality. These multiplex assays are complex systems that require a defined range of template input.

Investigator Quantiplex Pro Kits are a ready-to-use system for the detection of human and male DNA and parallel assessment of DNA degradation using quantitative real-time PCR. The kits provide fast and accurate quantification of human DNA from forensic database and casework samples. The kits contain reagents and a DNA polymerase for specific amplification of 4NS1C, a 91 bp proprietary region present on several autosomes of the human genome, and for detection of the specific PCR products on Applied Biosystems 7500 Real-Time PCR Systems or the Rotor-Gene Q System.

Information on the targets, amplicon lengths, channels, ploidy and copy numbers is summarized in the table “Targets, amplicon lengths and channels for Investigator Quantiplex Kits”.

Targets, amplicon lengths and channels for Investigator Quantiplex Kits

The kits are a ready-to-use system for the detection and quantification of human DNA using real-time PCR. See the table "Technical specifications" for more information. A set of template files has been developed to streamline the instrument setup and the analysis of the results of Investigator Quantiplex Kits on the Rotor-Gene Q (see table "Template files available for the Rotor-Gene Q").

Investigator Quantiplex Kits are for quantifying human and male DNA, and for determining if PCR inhibitors or DNA degradation are present and if further sample purification is needed. They are suitable for:

• Forensic database and casework samples
• Paternity testing samples
• Sexual assault samples
• Disaster victim identification and anthropological work
• Other human identity testing applications