Human identification is commonly based on the analysis of short tandem repeats (STRs), single nucleotide polymorphisms (SNPs) or deletion insertion polymorphisms (DIPs), depending on the demands of an examination or on the sample quality. These multiplex assays are complex systems that require a defined range of template input.
Investigator Quantiplex Pro Kits are a ready-to-use system for the detection of human and male DNA and parallel assessment of DNA degradation using quantitative real-time PCR. The kits provide fast and accurate quantification of human DNA from forensic database and casework samples. The kits contain reagents and a DNA polymerase for specific amplification of 4NS1C, a 91 bp proprietary region present on several autosomes of the human genome, and for detection of the specific PCR products on Applied Biosystems 7500 Real-Time PCR Systems or the Rotor-Gene Q System.
Information on the targets, amplicon lengths, channels, ploidy and copy numbers is summarized in the table “Targets, amplicon lengths and channels for Investigator Quantiplex Kits”.
Targets, amplicon lengths and channels for Investigator Quantiplex Kits
Kit |
Target |
Amplicon length |
Channel |
Ploidy |
Copy number |
Quantiplex |
Human target |
146 bp |
FAM |
Diploid |
Multi-copy |
Internal PCR control |
200 bp |
VIC |
N.A. |
Synthetic fragment |
Quantiplex HYres |
Human target |
146 bp |
FAM |
Diploid |
Multi-copy |
Human male target |
129 bp |
Cy5 |
Haploid |
Multi-copy |
Internal PCR control |
200 bp |
VIC |
N.A. |
Synthetic fragment |
Quantiplex Pro |
Human target, small autos. |
91 bp |
FAM |
Diploid |
Multi-copy |
Human target, large autos. |
353 bp |
TAMRA/Atto550 |
Diploid |
Multi-copy |
Human male target |
81 bp |
CY5/Atto647N |
Diploid |
Multi-copy |
Internal PCR control |
434 bp |
JOE |
N.A. |
Synthetic fragment |
Quantiplex Pro RGQ |
Human target, small autos. |
91 bp |
Yellow |
Diploid |
Multi-copy |
Human target, large autos. |
353 bp |
Red |
Diploid |
Multi-copy |
Human male, small Y |
81 bp |
Green |
Haploid |
Multi-copy |
Human male, large Y |
359 bp |
Orange |
Haploid |
Multi-copy |
Internal PCR control |
434 bp |
Crimson |
N.A. |
Synthetic fragment |