Phospho-Specific Antibodies

For detection of proteins containing phosphorylated serine or threonine residues

Features

  • Phosphorylated serine or threonine residue detection in any amino acid sequence
  • Cell-signaling studies without the need for radioactivity

Product Details

Each antibody is a mixture of anti-phosphoserine or anti-phosphothreonine antibodies. They are used to detect proteins with phosphorylated serine or threonine residues after western blotting.

Principle

PhosphoSerine and PhosphoThreonine Antibodies recognize and bind to phosphorylated serine and threonine residues, irrespective of surrounding amino acids. The use of chemiluminescent detection in conjunction with PhosphoSerine and PhosphoThreonine Antibodies is strongly recommended.

Procedure

The PhosphoProtein Handbook contains detailed protocols for detecting phosphorylated proteins using the PhosphoSerine Antibody Q5 and PhosphoThreonine Antibody Q7.

Applications

The PhosphoSerine Antibody Q5 and PhosphoThreonine Antibody Q7 highly suitable for SDS-PAGE and mass spectrometry for research in:

  • Proteomics
  • Cell signaling and apoptosis
  • Protein kinases and oncology
  • Immune disorders

Phospho-Specific Antibodies are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.

Comparison of QIAamp MinElute Virus Kits

Property PhosphoSerine Antibody Q5 PhosphoThreonine Antibody Q7
Detection Phosphorylated serine residues, irrespective of surrounding amino acids Phosphorylated threonine residues, irrespective of surrounding amino acids
Isotype(s) Mouse IgG1 and IgM Mouse IgG1
Form Lyophilized Lyophilized
Working concentration 1/100–1/200 dilution of antibody stock solution 1/100–1/200 dilution of antibody stock solution
Working solution 100–200 ml 100–200 ml

Resources

Kit Handbooks (1)
For purification and detection of phosphorylated
proteins from eukaryotic cells and tissues

FAQ

Do you provide control proteins for use with the PhosphoProtein Purification Kit?

No, we do not currently provide controls for this kit. BSA can be used as a negative control, since it is not phosphorylated. Use only high-purity grade BSA without any residual phopholipids.

We recommend casein or ovalbumin as a positive control. These proteins can be detected using our PhosphoSerine and PhosphoThreonine Antibody.

FAQ ID -596
How was it determined that the PhosphoSerine Antibody will bind phosphoserines only?
This was determined via epitope mapping using peptides with phosphoserine in different surroundings.
FAQ ID -579
How is an antibody epitope determined?
This is done via epitope mapping. The antibody in question is first tested against different protein fragments and peptides. Once the amino acids detected by the antibody are determined, the surrounding amino acids can be exchanged to reveal the influence of the environment on the detection of a specific epitope.
FAQ ID -593
Will the PhosphoSerine antibody recognize Serine residues if the neighboring residues are Leucine or Isoleucine?

Yes. The PhosphoSerine Antibody will detect phosphorylated Serine residues, irrespective of surrounding amino acids. 

FAQ ID -644