Penta·His Alexa Fluor Conjugates

For sensitive, direct immunofluorescent detection of His-tagged proteins, with an emission peak at 488 and 647 nm

Features

  • Highly specific and sensitive detection of His-tagged proteins
  • Direct detection without the need for a secondary antibody
  • Broad instrument compatibility and high photostability

Useful Resources

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Penta·His Alexa Fluor 488 Conjugate

Cat. No. / ID: 35310

125 μl Penta·His Alexa Fluor 488 Conjugate, 200 μg/ml
$263.00
Emission at
488 nm
647 nm
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Penta·His Alexa Fluor Conjugates are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Product Details

Penta·His Alexa Fluor 488 Conjugate and Penta·His Alexa Fluor 647 Conjugate (His tag Alexa Fluor conjugates) are for sensitive, direct immunofluorescent detection of recombinant proteins carrying His tags (consisting of at least five consecutive His residues) at 488 and 647 nm. Penta·His Alexa Fluor Conjugates are used for applications including immunocytochemistry, protein localization and targeting studies and fluorescence-activated cell sorting (FACS).

Performance

Features Penta·His Alexa Fluor 488 Conjugate Penta·His Alexa Fluor 647 Conjugate
Detection Five consecutive histidine residues Five consecutive histidine residues
Antibody Isotype Mouse IgG1 Mouse IgG1
Cross-reactivity Negligible with crude E. coli, yeast, mammalian or insect cell lysates Negligible with crude E. coli, yeast, mammalian or insect cell lysates
Form Stabilized stock solution Stabilized stock solution
Working solution Depends on application Depends on application

Principle

Penta·His Antibodies are available as Alexa Fluor 488 and 647 conjugates (His tag Fluor 647, His tag Fluor 488), giving a range of highly specific reagents whose emission wavelengths cover a wide portion of the visible spectrum for Penta·His immunofluorescent detection.

Procedure

The QIAexpress, Detection and Assay Handbook, supplied with all QIAexpress detection and assay products, contains detailed example protocols and guidelines for designing and performing assays. 

Applications

The Penta·His Alexa Fluor 488 Conjugate and Penta·His Alexa Fluor 647 Conjugate can be used in immunofluorescence procedures such as flow cytometry, confocal laser microscopy, immunocytochemistry, immunohistochemistry, protein localization and targeting studies.

Features Specifications
Detection Direct
Epitope detected HHHHH
Tag His tag

 

Resources

Safety Data Sheets (1)
Download Safety Data Sheets for QIAGEN product components.
Kit Handbooks (1)
For Anti·His Antibodies, Anti·His HRP Conjugates, Penta·His™ Alexa Fluor® Conjugates, Penta·His Biotin Conjugate, Ni-NTA Conjugates, Tag·100™ Antibody, Streptavidin–R-PE, 6xHis Protein Ladder, Ni-NTA HisSorb™ Strips and Plates 

Publications

Cleavage of the plasma membrane Na+/Ca2+ exchanger in excitotoxicity.
Bano D; Young KW; Guerin CJ; Lefeuvre R; Rothwell NJ; Naldini L; Rizzuto R; Carafoli E; Nicotera P;
Cell; 2005; 120 (2):275-85 2005 Jan 28 PMID:15680332