BioMag Goat Anti-Mouse/Anti-Rat IgG

For magnetic cell separation and magnetocapture, immunoassays, and binding of immunoglobulins

Features

  • For use with mouse or rat IgG primary antibodies
  • Can be used as secondary antibodies in enzyme immunoassays and radioassays 
  • Negative selection separates cells of interest from heterogeneous cell populations 
BioMag Goat Anti-Rat IgG (500 ml)

Cat. No. / ID: 310107

BioMag goat anti-rat IgG secondary antibody suspension (1 mg/ml)
$1,569.00
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Product Details

BioMag Goat Anti-Mouse IgG and BioMag Goat Anti-Rat IgG are standard BioMag particles coated with polyclonal goat anti-mouse or anti-rat IgG antibodies and are highly suited for use in cell sorting methods where a mouse or rat IgG antibody is used as a primary antibody. BioMag Goat Anti-Mouse IgG and BioMag Goat Anti-Rat IgG can be used to separate the cells of interest from a heterogeneous cell population using negative selection. BioMag Goat Anti-Mouse IgG and BioMag Goat Anti-Rat IgG can also be used as secondary antibodies in enzyme immunoassays and radioassays that utilize a mouse or rat IgG primary monoclonal antibody.

Resources

Kit Handbooks (2)

Publications

Gammadelta T cells promote a Th1 response during coxsackievirus B3 infection in vivo: role of Fas and Fas ligand.
Huber S; Shi C; Budd RC;
J Virol; 2002; 76 (13):6487-94 2002 Jul PMID:12050361
Multimeric connexin interactions prior to the trans-Golgi network.
Das Sarma J; Meyer RA; Wang F; Abraham V; Lo CW; Koval M;
J Cell Sci; 2001; 114 (Pt 22):4013-24 2001 Nov PMID:11739633
Cadherins mediate intercellular mechanical signaling in fibroblasts by activation of stretch-sensitive calcium-permeable channels.
Ko KS; Arora PD; McCulloch CA;
J Biol Chem; 2001; 276 (38):35967-77 2001 Jul 20 PMID:11466312
Filarial antigens impair the function of human dendritic cells during differentiation.
Semnani RT; Sabzevari H; Iyer R; Nutman TB;
Infect Immun; 2001; 69 (9):5813-22 2001 Sep PMID:11500459
Impaired apoptosis, extended duration of immune responses, and a lupus-like autoimmune disease in IEX-1-transgenic mice.
Zhang Y; Schlossman SF; Edwards RA; Ou CN; Gu J; Wu MX;
Proc Natl Acad Sci U S A; 2002; 99 (2):878-83 2002 Jan 8 PMID:11782530

FAQ

I used to buy BioMag particles from a company by another name? Are your BioMag particles the same thing?
Our supplier, Polysciences, Inc. purchased the exclusive manufacturing rights and procedures for making BioMag from PE Biosystems. Polysciences, Inc. is now the manufacturer of this product line, while QIAGEN owns the exclusive distribution rights. Since the purchase, many new BioMag products have been added and improvements have been made to the existing BioMag particles. QIAGEN performs constant R&D on the products to improve the existing products and support and develop new products.
FAQ ID -263
Can BioMag be used for positive and negative selection?
BioMag can be used for both positive and negative selection. However, QIAGEN recommends negative selection for most cell separation applications. During negative selection, BioMag particles are attached to the unwanted component of the mixture and the cells or molecules of interest are left behind after the magnetic separation has been performed. The resulting supernatant is then enriched for the target cells/molecules. QIAGEN recommends this procedure for cell separation to achieve naïve, untouched target cells. When BioMag is used to pull out only the target cells/molecules out of solution and the supernatant is discarded, this is referred to as positive selection. In positive selection, only the target cells/molecules will be attached to the BioMag and all other contaminating cells/molecules are discarded resulting in a pure suspension of the target components. This procedure can be performed using BioMags, however the BioMag particles will remain attached to the target analyte or cell. Although this is not a problem for molecular biology applications (e.g. subsequent RNA or DNA extraction from cells), the presence of BioMag particles may interfere with FACS signal / interpretation if positively selected cells are used in fluorescent flow cytometry.
FAQ ID -269
For how long are BioMag Particles stable?
The BioMag particles can be kept at 4°C at least until the expiration date without showing any reduction in performance. In general this is two years after production.
FAQ ID -262
Are BioMag particles stable in other solvents?
BioMag particles are packaged in a distilled water/EDTA solution with 0.08% sodium azide and BSA. BioMag particles has been used in various coupling buffers at pH’s ranging from 5.5 to 8.0. Other than the various buffers used in the coupling procedures, BioMag particles has not been tested in any other organic solvents or at radical pH values. While BioMag particles has been used in a wide variety of conditions, it is suggested that the user determine the suitability of BioMag particles for their particular application.
FAQ ID -267
If I want to use BioMag particles, do I have to buy a QIAGEN magnet too or can I use one that I already have?
For maximum performance, BioMag particles should be used with one of QIAGEN magnets. Since BioMag particles are so small, they require a strong magnetic field in order to separate them in a timely fashion. The BioMag magnets are rare earth (Neodymium-Iron-Boron) magnets embedded into plastic, with magnetic strengths ranging from 27 megagauss Oersteds to 35 megagauss Oersteds depending on the magnet. Strong magnets from alternative suppliers may also provide a suitable alternative.
FAQ ID -266
Can I use BioMag particles with any specific primary antibody?
Yes. BioMag secondary antibody particles can be used with a wide variety of antibodies. They are produced by covalently attaching polyclonal antibodies from various species to BioMag particles. They are available with Anti-Rabbit, Anti-Rat and Anti-Mouse coatings.
FAQ ID -273
How magnetically responsive is BioMag?
BioMag particles are superparamagnetic in that they have no magnetic memory and will readily resuspend if the magnetic force is removed. They have over 90% magnetite content and have a magnetization of 25-35 EMU/g (Electromagnetic Units).
FAQ ID -265
My BioMag is about to expire, is it still any good?
Since the BioMag base particle is simply an Iron Oxide particle with a coating, the particle itself is very stable. The primary aspect of a BioMag particle that can degrade over time is the antibodies/proteins themselves. The antibody can loose its effectiveness over long periods of time or the protein may degrade. Although the expiration dates on BioMag are set at 2 years, if stored properly at 4°C and kept tightly sealed to prevent bacterial contamination, BioMag will likely perform properly even after the expiry date has passed. But please understand that we only can guarantee for the performance of the BioMag particle until the expiry date.
FAQ ID -268